Effect of Different Levels of Cellulose in the Diet on the Proteolytic Activity of the Pancreas in Broiler Chickens

Bogułsawska-Tryk, Monika

doi:10.3409/173491605775789353

Cited by 13 publications

(13 citation statements)

References 25 publications

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“…Furthermore, they reported significant increases in both pepsin and intestinal aminopeptidase activities in layer pullets offered control diets supplemented with 1.5% levels of dietary Arbocel ® . Although the present work did not test gut enzymatic activity, the improved protein and amino acid digestibility coefficients observed are strongly suggestive of improved digestion and assimilation of nutrients (Boguslawska-Tryk, 2005;Yokhana et al, 2016).…”

Section: Resultsmentioning

confidence: 59%

“…Correction to zero nitrogen balance was done using the factor 8, 220 cal/g of uric acid nitrogen (Hill and Anderson, 1958). Feed and excreta samples were also analyzed for proximate chemical composition following the methods of the AOAC (1998). The concentration of amino acids, including total sulfur amino acids (TSAA) and tryptophan in the test feed and excreta samples, was determined using HPLC 2690 (Waters Co., Milford, MA).…”

Section: Rooster Digestibility Trialmentioning

confidence: 99%

“…The concentration of amino acids, including total sulfur amino acids (TSAA) and tryptophan in the test feed and excreta samples, was determined using HPLC 2690 (Waters Co., Milford, MA). Except for tryptophan, all amino acids in feed and excreta samples were quantified after acid hy- drolysis in 6 N HCl using the 982.30E and 982.30Ea methods of AOAC (1998) in the presence of phenol at 110℃ for 24 h. For TSAA determination, samples were subjected to performic acid oxidation before acid hydrolysis as in AOAC (1998) official method 982.30Eb. Tryptophan was quantified after sample hydrolysis in barium hydroxide at 120℃ for 16 h according to AOAC (1998) official method 982.30Ec.…”

Section: Rooster Digestibility Trialmentioning

confidence: 99%

“…Except for tryptophan, all amino acids in feed and excreta samples were quantified after acid hy- drolysis in 6 N HCl using the 982.30E and 982.30Ea methods of AOAC (1998) in the presence of phenol at 110℃ for 24 h. For TSAA determination, samples were subjected to performic acid oxidation before acid hydrolysis as in AOAC (1998) official method 982.30Eb. Tryptophan was quantified after sample hydrolysis in barium hydroxide at 120℃ for 16 h according to AOAC (1998) official method 982.30Ec. All amino acids, except for tryptophan, were derivatized using the AccQ-Tag method, whereas all amino acids were separated by Waters HPLC column (AccQ-Tag 3.9×150) and then identified and quantified using Waters 474 scanning fluorescence detector at a range between 285 and 345 nm for tryptophan and 250 to 395 nm for the other amino acids (Waters Co., Milford, MA).…”

Section: Rooster Digestibility Trialmentioning

confidence: 99%

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Lignocellulose Improves Protein and Amino Acid Digestibility in Roosters and Egg Hatchability in Broiler Breeders

et al. 2017

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The present work assessed the effect of supplementation of 0.8% dietary Arbocel ® RC Fine, a readily available commercial lignocellulose, to poultry feed. In a complete randomized design using 36 individually caged mature dubbed Hy-Line roosters (aged 55 weeks) grouped in 4 treatments with 9 birds per treatment, a digestibility trial was performed to determine apparent and true metabolizable energy values along with digestibility coefficients of protein and amino acid in Arbocel ® containing diets. Results showed that 0.8% Arbocel ® supplemented diets improved protein digestibility by 6% (P＜0.05). Additionally, Arbocel ® caused an increase in apparent and true amino acid digestibility in roosters when compared to control diets and controls with 0.8% wheat bran (WB) supplementation. In a second experiment, 26,000 layers and 2,600 roosters aged 33 weeks (Ross 308 broiler breeder strain) were maintained in 6 poultry houses at a commercial breeding farm, with an average of 4330 layers and 433 roosters per house. Performance, egg grade, and hatchability rate were assessed over a post peak period of 6 months. Compared to the control group fed the 0.8% WB diet, the 0.8% lignocellulose dietary supplementation resulted in a decrease (P＜0.05) in percent infertility leading to an average increase of 4.07% (P＜0.05) in egg hatchability. The Arbocel ® fed group had 3.8 more eggs per housed hen compared to control birds. Overall, Arbocel ® supplementation at 0.8% resulted in the production of 5.7 more saleable chicks per housed hen during the 6 months trial, a sizeable profit to the farmer.

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Section: Resultsmentioning

confidence: 59%

Section: Rooster Digestibility Trialmentioning

confidence: 99%

Section: Rooster Digestibility Trialmentioning

confidence: 99%

Section: Rooster Digestibility Trialmentioning

confidence: 99%

See 2 more Smart Citations

Lignocellulose Improves Protein and Amino Acid Digestibility in Roosters and Egg Hatchability in Broiler Breeders

et al. 2017

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“…This effect was associated with the significant increase in amylase activity and bile acid concentration in jejunal contents (Hetland et al., ). Moreover, Bogusławska‐Tryk () reported that inclusion of even minor amounts of pure cellulose (0.5–0.7%) into the broiler diet during the rearing period markedly enhanced the content of protein in the pancreas gland and significantly elevated the proteolytic enzymes in pancreatic homogenates. It has been proved that the effect of dietary fibre on secretion of pancreatic enzymes is linked to its direct impact on releasing the hormones of alimentary tract, mainly cholecystokinin (Sánchez et al., ), which in turn stimulates secretion of all pancreas enzymes, including lipolytic ones, and regulates bile flow (Krogdahl, ) facilitating fat digestion.…”

Section: Discussionmentioning

confidence: 99%

Effect of dietary lignocellulose on ileal and total tract digestibility of fat and fatty acids in broiler chickens

Bogusławska–Tryk

Piotrowska

Szymeczko

et al. 2016

Animal Physiology Nutrition

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The study was conducted to determine the effect of a lignocellulose supplemented diet on apparent ileal and total tract digestibility of fat and fatty acids (FA) in broiler chickens. A total of 48 21-day-old male Ross 308 chickens were divided into four treatment groups (n = 12) with six replicates per treatment. From 21 to 42 days of age, the broilers were fed experimental diets varied in the amount of lignocellulose: 0%, 0.25%, 0.5% and 1%. Total excreta were gathered during the last 3 days of the feeding trial and digesta was collected from the ileum at 42 days of the bird age. Digestibility was determined by the indicator method. The ether extract content in diet/digesta/excreta was determined by the gravimetric method, and fatty acid methyl esters were analysed by gas chromatography-mass spectrometry. Fat digestibility measured to the end of the small intestine and in the whole gastrointestinal tract in birds was high and exceeded 90% and 87% respectively. Addition of lignocellulose (1%) increased (p< 0.05) ileal fat digestibility but had no significant effect on total tract fat digestion. Absorption of total fatty acids (TFA) as well as myristic (C14:0), palmitoleic (C16:1) and α-linolenic (C18:3n-3) acids, estimated by both methods, was significantly higher in birds fed the diets supplemented with lignocellulose, especially at a dose of 1%. Total tract absorption of some dietary polyunsaturated fatty acids (PUFA) (C20:2, C20:4n-6) was significantly lower from diet supplemented with 0.5% and 0.25% lignocellulose. There was observed a decrease in apparent digestibility of fat and most examined fatty acids, when measured between terminal ileum and total gastrointestinal tract. The results suggest that lignocellulose can affect digestion and FA absorption in broilers but, as the effect of lignocellulose was not studied previously, further investigations are necessary to confirm the results of the present experiment.

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Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn

et al. 2018

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BackgroundMeasures to improve bird performance have been sought due to the imminent phase out of in-feed antibiotics in poultry and continued demand for higher poultry feeding efficiency. Increasing grain particle size and dietary fibre may improve gizzard function, digestive efficiency and nutrient absorption. This study was conducted to evaluate the effect increased particle size of corn and inclusion of sugarcane bagasse (SB) on mRNA expression of genes encoding digestive enzymes and nutrient transporters in broilers.ResultsA total of 336 day-old Ross 308 males were assigned in a 2 × 2 factorial arrangement of treatments with corn particle size - coarse 3576 μm or fine 1113 μm geometric mean diameter, and SB - 0 or 2% inclusion. Feed conversion ratio (FCR), weight gain and feed intake were measured from d 0–10 and d 10–24. The relative gizzard weight and mRNA expression of genes encoding digestive enzymes and intestinal nutrient transporters were measured on d 24. During d 10–24, a particle size × SB interaction was observed for FCR (P < 0.01), where birds fed coarsely ground corn (CC) with 2% SB had lower FCR than those fed CC without SB. A particle size × SB interaction was observed for both expression of pepsinogen A and C (P < 0.01) which were negatively correlated with FCR on d 24. Addition of 2% SB upregulated pepsinogen A and C only in CC fed birds. Further, 2% SB also upregulated pancreatic amylase (AMY2A) and intestinal cationic amino acid transporter-1 (CAT1). Inclusion of dietary CC upregulated duodenal amino peptidase N (APN), jejunal alanine, serine, cysteine and threonine transporter-1 (ASCT1), and ileal peptide transporter-2 (PepT2).ConclusionThese results suggest that both SB and coarse particle size modulate expression of genes encoding important digestive enzymes and nutrient transporters and thus are directly related to bird performance. These findings provide insights into the combination effects of dietary fiber and particle size in the future management of broiler feeding.

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Effect of Different Levels of Cellulose in the Diet on the Proteolytic Activity of the Pancreas in Broiler Chickens

Cited by 13 publications

References 25 publications

Lignocellulose Improves Protein and Amino Acid Digestibility in Roosters and Egg Hatchability in Broiler Breeders

Lignocellulose Improves Protein and Amino Acid Digestibility in Roosters and Egg Hatchability in Broiler Breeders

Effect of dietary lignocellulose on ileal and total tract digestibility of fat and fatty acids in broiler chickens

Upregulation of genes encoding digestive enzymes and nutrient transporters in the digestive system of broiler chickens by dietary supplementation of fiber and inclusion of coarse particle size corn

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