Effect of different initiators on temperature‐sensitive monolithic columns and application in online enrichment of β‐sitosterol

Abstract

Three temperature‐sensitive monolithic columns were prepared by atom transfer radical polymerization and oxidation–reduction method using the initiator systems of CCl4/FeCl2, BPO/DMA, and APS/TEMED, respectively. The three monoliths were characterized by scanning electron microscopy, nitrogen adsorption, and thermogravimetric analysis. The obtained monolithic columns were used as on‐line solid phase extraction sorbent coupled with high‐performance liquid chromatography for the enrichment of β‐sitosterol. By comparing some influencing factors on the adsorption, the optimum temperature‐sensitive monolithic column which was initiated by CCl4/FeCl2 was selected for enrichment of β‐sitosterol from plant oil. The maximum adsorption capacity of the monolith for β‐sitosterol was 10.0031 mg/g. The limit of detection and limit of quantitation were 0.039 and 0.13 mg/mL, respectively. The recovery was in the range of 90.21–98.26%. The results showed that the monolith had better selectivity for β‐sitosterol and could be used for enrichment of β‐sitosterol in food samples. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47683.

Functionalized monolithic columns: Recent advancements and their applications for high-efficiency separation and enrichment in food and medicine

“…For instance, ß-sitosterol in plant oil samples has significant antiinflammatory and antitumor effects, but it is difficult to detect. A new monolithic column was prepared and a new method was developed for online enrichment and determination of ßsitosterol in plant oil samples by online SPE combined with HPLC (Guo et al, 2019;. Phenolic acids have excellent antioxidant activity.…”