Effect of different initial low pH conditions on biogas production, composition, and shift in the aceticlastic methanogenic population

Ali, Salman Syed; Hua, Bin; Huang, Jinhui Jeanne; Droste, Ronald L.; Zhou, Qixing; Zhao, Weixin; Chen, Lu

doi:10.1016/j.biortech.2019.121579

Cited by 37 publications

(25 citation statements)

References 36 publications

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“…The more negative Δ G Overall value in this scenario indicates a potential increase in the driving force to carry out the syntrophic reaction. Nonetheless, this might be compromised by physiological limitations and enhanced toxicity effects 37 observed at decreased pH levels, particularly in the case of methanogenic populations. 38 In consequence, bioenergetics does not suffice to elucidate the detrimental effects observed on the syntrophic conversions if pH is considered as the main explanatory variable.…”

Section: Resultsmentioning

confidence: 99%

Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Ceron-Chafla

Kleerebezem

Rabaey

et al. 2020

Environ. Sci. Technol.

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Simultaneous digestion and in situ biogas upgrading in high-pressure bioreactors will result in elevated CO 2 partial pressure (pCO 2 ). With the concomitant increase in dissolved CO 2 , microbial conversion processes may be affected beyond the impact of increased acidity. Elevated pCO 2 was reported to affect the kinetics and thermodynamics of biochemical conversions because CO 2 is an intermediate and end-product of the digestion process and modifies the carbonate equilibrium. Our results showed that increasing pCO 2 from 0.3 to 8 bar in lab-scale batch reactors decreased the maximum substrate utilization rate ( r smax ) for both syntrophic propionate and butyrate oxidation. These kinetic limitations are linked to an increased overall Gibbs free energy change (Δ G Overall ) and a potential biochemical energy redistribution among syntrophic partners, which showed interdependence with hydrogen partial pressure (pH 2 ). The bioenergetics analysis identified a moderate, direct impact of elevated pCO 2 on propionate oxidation and a pH-mediated effect on butyrate oxidation. These constraints, combined with physiological limitations on growth exerted by increased acidity and inhibition due to higher concentrations of undissociated volatile fatty acids, help to explain the observed phenomena. Overall, this investigation sheds light on the role of elevated pCO 2 in delicate biochemical syntrophic conversions by connecting kinetic, bioenergetic, and physiological effects.

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Section: Resultsmentioning

confidence: 99%

Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Ceron-Chafla

Kleerebezem

Rabaey

et al. 2020

Environ. Sci. Technol.

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“…Typically, raw biogas is composed of CH 4 (60–70%), CO 2 (30–40%), H 2 O (5–10%), and, depending on the biomass matrix, trace amounts of other species such as H 2 S (0.15–3%), NH 3 (<1%), CO (<0.6%), siloxanes, carbonyls, terpenes, and aromatic or halogenated compounds. − Biogas upgrading for increasing its calorific value involves specific steps, starting with H 2 O condensation, desulfurization (e.g., removal of toxic and corrosive H 2 S), and CO 2 sequestration based on different universally established and commonly used technologies including physisorption and/or chemisorption, membrane or cryogenic separation, and by chemical or biological treatment. ,− …”

Section: Introductionmentioning

confidence: 99%

Adsorption of Hydrogen Sulfide at Low Temperatures Using an Industrial Molecular Sieve: An Experimental and Theoretical Study

et al. 2021

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In the work presented herein, a joint experimental and theoretical approach has been carried out to obtain an insight into the desulfurization performance of an industrial molecular sieve (IMS), resembling a zeolitic structure with a morphology of cubic crystallites and a high surface area of 590 m 2 g –1 , with a view to removing H 2 S from biogas. The impact of temperature, H 2 S inlet concentration, gas matrix, and regeneration cycles on the desulfurization performance of the IMS was thoroughly probed. The adsorption equilibrium, sorption kinetics, and thermodynamics were also examined. Experimental results showed that the relationship between H 2 S uptake and temperature increase was inversely proportional. Higher H 2 S initial concentrations led to lower breakpoints. The presence of CO 2 negatively affected the desulfurization performance. The IMS was fully regenerated after 15 adsorption/desorption cycles. Theoretical studies revealed that the Langmuir isotherm better described the sorption behavior, pore diffusion was the controlling step of the process (Bangham model), and that the activation energy was 42.7 kJ mol –1 (physisorption). Finally, the thermodynamic studies confirmed that physisorption predominated.

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“…Regarding the archaeal sequences, representatives were found for the genera Methanosaeta and Methanobacterium respectively fof the phyla of the Halobacterota and Euryarchaeota phyla in the starter, which have already been related to other processes of anaerobic digestion and biogas production. Representatives of the Methanosaeta genus maintained their dominance over other methanogenic groups in a study where acetoclastic methanogen groups able to act at low pH were acclimated to replace the use of NaOH to regulate buffer pH, a procedure that can inhibit methanogenic microorganisms (Ali et al 2019). The acetoclastic methanogenic genus Methanosaeta has also been observed in other studies to improve biogas production (Zamorano et al 2020; Chen et al 2017).…”

Section: Metabarcoding Analyzesmentioning

confidence: 99%

Cultured and Uncultured Microbial Community Associated With Biogas Production

Ottoni¹,

Bernal²,

Marteres³

et al. 2021

Preprint

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The search for sustainable development has led countries around the world to seek the improvement of technologies that use renewable energy sources. One of the alternatives in the production of renewable energy comes from the use of waste including urban solids, animal excrement from livestock and biomass residues from agro-industrial plants. These materials may be used in the production of biogas, making its production highly sustainable and environmentally friendly, in addition to reducing public expenses for the treatment of those wastes. The present study evaluated the cultivated and uncultivated microbial community from a substrate (starter) used as an adapter for biogas production in anaerobic digestion processes. 16S rDNA metabarcoding revealed domain of bacteria belonging to the phyla Firmicutes, Bacteroidota, Chloroflexi and Synergistota. The methanogenic group was represented by the phyla Halobacterota and Euryarchaeota. Through 16S rRNA sequencing analysis of isolates recovered from the starter culture, the genera Rhodococcus, Vagococcus, Lysinibacillus, Niallia, Priestia, Robertmurraya, Luteimonas and Proteiniclasticum were recovered, groups that were not observed in the metabarcoding data. The groups mentioned are involved in the metabolism pathways of sugars and other compounds derived from lignocellulosic material, as well as in anaerobic methane production processes. The results demonstrate that culture-dependent approaches, such as isolation and sequencing of isolates, as well as culture-independent studies, such as the Metabarcoding approach, are complementary methodologies that, when integrated, provide robust and comprehensive information about the microbial communities involved in various processes, including the production of biogas in anaerobic digestion processes.

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Effect of different initial low pH conditions on biogas production, composition, and shift in the aceticlastic methanogenic population

Cited by 37 publications

References 36 publications

Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Adsorption of Hydrogen Sulfide at Low Temperatures Using an Industrial Molecular Sieve: An Experimental and Theoretical Study

Cultured and Uncultured Microbial Community Associated With Biogas Production

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Effect of different initial low pH conditions on biogas production, composition, and shift in the aceticlastic methanogenic population

Cited by 37 publications

References 36 publications

Direct and Indirect Effects of Increased CO2 Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Direct and Indirect Effects of Increased CO2 Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Adsorption of Hydrogen Sulfide at Low Temperatures Using an Industrial Molecular Sieve: An Experimental and Theoretical Study

Cultured and Uncultured Microbial Community Associated With Biogas Production

Contact Info

Product

Resources

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Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion

Direct and Indirect Effects of Increased CO₂ Partial Pressure on the Bioenergetics of Syntrophic Propionate and Butyrate Conversion