Effect of different hepatitis B infection status on the prognosis of active lupus nephritis treated with immunosuppression: a retrospective analysis of 177 patients

Fang, Jing; Li, Wenge; Tan, Min; Peng, Xiang-xin; Tan, Zhao; Wang, Wenbo

doi:10.1111/1756-185x.13313

Cited by 6 publications

(1 citation statement)

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“…Frequency of Hepatitis B Markers in Systemic Lupus Erythematosus Patients in Iran nested PCR and Real time PCR (p=0.05). It was reported that among 177 adults with active lupus nephritis, 68 cases were positive with OBI with undetectable HBV DNA (Fang et al, 2018). The results of phylogenic analysis revealed that all three isolated HBV DNA (K886716, MK886717, and MK886718) were clustered with genotype D1 isolated from Turkey (AB674418.1).…”

Section: Author Contribution Statementmentioning

confidence: 96%

Frequency of Hepatitis B Markers in Systemic Lupus Erythematosus Patients in Iran

Makvandi

Pour

Teimoori

et al. 2022

Asian Pac J Cancer Prev

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Background: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease. Hepatitis B virus is the causative agent for chronic, acute, cirrhosis, and hepatocellular carcinoma. SLE patients with chronic or occult hepatitis B infection undergoing immunosuppressive drugs may become reactive and develop fatal hepatitis. Therefore, this study was conducted to determine HBV markers in SLE patients before the administration of immunosuppressive drugs in Ahvaz city, Iran. Materials and methods: The sera of 92 SLE patients were tested for HBs Ag and anti-HBc using ELISA, HBV DNA (by Nested PCR) testes. Real-time PCR was performed for the patients with positive anti-HBc and negative HBsAg. The positive HBV DNA samples were checked for HBV genotype and HBV subtypes. Results: Among the 92 SLE patients, three (3.3%) were males and 89 (96.7%) females . The patients' ages ranged from 14 to 70 years [mean age of 38.9±10.1]. Three of 92 (3.26%) subjects [2/3 males and 1/89 female] were positive for HBsAg, anti-HBc Ab, and HBV DNA detected with PCR (p=0.000003)]. Five of 89 (5.61%) subjects [1 male and 4/88 females were only positive for anti-HBc and negative for HBs Ag, HBV DNA(PCR) using Real-time PCR (p=0.05). The results of the nucleotide data and phylogenetic tree showed all three HBV patients were genotype D1. The results of amino acid sequencing revealed all three HBV patients were HBV subtype ayw2. Conclusion: This study proved that 3.26% of SLE patients were positive for overt HBV infection (positive for anti-HBc, HBsAg and HBV-DNA using PCR). All the three isolated HBV were genotype D1 and subtype ayw2. The fact that 5.61% of the patients were only positive for anti-HBc characterized the occult hepatitis B infection (OBI) although further investigation is needed. To prevent HBV or OBI reactivation for SLE patients before immunosuppression treatment, HBV markers including anti-HBc, HBsAg, HBV-DNA should be implemented using PCR and Real-time PCR .

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Section: Author Contribution Statementmentioning

confidence: 96%