Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering

Bosetti, Michela; Boccafoschi, Francesca; Leigheb, Massimiliano; Cannas, M.

doi:10.1016/j.bioeng.2007.08.019

Cited by 57 publications

(32 citation statements)

References 24 publications

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“…TGF-βs and FGF-2, -4, and -6 have been proven to be inducers of osteoblast proliferation (a higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase (ALP) activity and osteoblast mineralization 36, indicating potential application for in vitro bone growth induction in bone tissue engineering. In addition, FGF acts downstream of TGF-β signaling in regulating CNC cell proliferation and exogenous FGF-2 rescues the cell proliferation defect in the frontal primordium of Tgfbr2 mutants, demonstrating the biological significance of the TGF-β-mediated FGF signaling cascade in regulating frontal bone development 37.…”

Section: Tgf-β Signaling In Osteoblast Differentiation and Bone Formamentioning

confidence: 99%

TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation

Chen¹,

Deng²,

Li³

2012

Int. J. Biol. Sci.

1,444

1,205

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Transforming growth factor-beta (TGF-β)/bone morphogenic protein (BMP) signaling is involved in a vast majority of cellular processes and is fundamentally important throughout life. TGF-β/BMPs have widely recognized roles in bone formation during mammalian development and exhibit versatile regulatory functions in the body. Signaling transduction by TGF-β/BMPs is specifically through both canonical Smad-dependent pathways (TGF-β/BMP ligands, receptors and Smads) and non-canonical Smad-independent signaling pathway (e.g. p38 mitogen-activated protein kinase pathway, MAPK). Following TGF-β/BMP induction, both the Smad and p38 MAPK pathways converge at the Runx2 gene to control mesenchymal precursor cell differentiation. The coordinated activity of Runx2 and TGF-β/BMP-activated Smads is critical for formation of the skeleton. Recent advances in molecular and genetic studies using gene targeting in mice enable a better understanding of TGF-β/BMP signaling in bone and in the signaling networks underlying osteoblast differentiation and bone formation. This review summarizes the recent advances in our understanding of TGF-β/BMP signaling in bone from studies of genetic mouse models and human diseases caused by the disruption of TGF-β/BMP signaling. This review also highlights the different modes of cross-talk between TGF-β/BMP signaling and the signaling pathways of MAPK, Wnt, Hedgehog, Notch, and FGF in osteoblast differentiation and bone formation.

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Section: Tgf-β Signaling In Osteoblast Differentiation and Bone Formamentioning

confidence: 99%

TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation

Chen¹,

Deng²,

Li³

2012

Int. J. Biol. Sci.

1,444

1,205

View full text Add to dashboard Cite

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“…Immunomodulatory and anti-inflammatory agents, such as selective anti-cytokine therapies, corticosteroids and non-steroidal anti-inflammatory drugs, are used to direct specific effects on the regeneration and resorption pathways during bone healing [25,26]. Additionally, the use of parathyroidal hormone (PTH), growth hormone, steroids, calcitonin and vitamin D in systemic applications has also been shown to advance bone healing through stimulating osteogenesis, angiogenesis and osteoblast differentiation [27][28][29][30]. Various combinations of biomolecules have also been extensively evaluated in pre-clinical models with mostly positive results [27,[31][32][33][34][35][36][37].…”

Section: Biomoleculesmentioning

confidence: 99%

The rational use of animal models in the evaluation of novel bone regenerative therapies

Perić¹,

Dumić-Čule²,

Grčević³

et al. 2015

Bone

125

116

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“…It is most likely that the mesenchymal stem cells from bone marrow aspirates, which drive the normal bone remodeling and regeneration, are an excellent source of cells for bone repair. Studies have shown that the cell culture substrate [24, 25], and the growth factors supplemented to cell culture medium [26-28] help maintain the differentiation potential of these cells during expansion. The need to utilize the right cell phenotype for engineering of human tissues is widely recognized, but the exact phenotypic characteristics are not always well defined.…”

Section: Tissue-engineered Bone Graftsmentioning

confidence: 99%

Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance

Fröhlich

Grayson

Wan

et al. 2008

CSCR

293

239

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The tremendous need for bone tissue in numerous clinical situations and the limited availability of suitable bone grafts are driving the development of tissue engineering approaches to bone repair. In order to engineer viable bone grafts, one needs to understand the mechanisms of native bone development and fracture healing, as these processes should ideally guide the selection of optimal conditions for tissue culture and implantation. Engineered bone grafts have been shown to have capacity for osteogenesis, osteoconduction, osteoinduction and osteointegration - functional connection between the host bone and the graft. Cells from various anatomical sources in conjunction with scaffolds and osteogenic factors have been shown to form bone tissue in vitro. The use of bioreactor systems to culture cells on scaffolds before implantation further improved the quality of the resulting bone grafts. Animal studies confirmed the capability of engineered grafts to form bone and integrate with the host tissues. However, the vascularization of bone remains one of the hurdles that need to be overcome if clinically sized, fully viable bone grafts are to be engineered and implanted. We discuss here the biological guidelines for tissue engineering of bone, the bioreactor cultivation of human mesenchymal stem cells on three-dimensional scaffolds, and the need for vascularization and functional integration of bone grafts following implantation.

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Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering

Cited by 57 publications

References 24 publications

TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation

TGF-β and BMP Signaling in Osteoblast Differentiation and Bone Formation

The rational use of animal models in the evaluation of novel bone regenerative therapies

Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance

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