Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells

Mastrogiacomo, Maddalena; Cancedda, Ranieri; Quarto, Rodolfo

doi:10.1053/joca.2001.0442

Cited by 137 publications

(110 citation statements)

References 19 publications

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“…The best method to obtain strong cell-to-cell interaction is to culture MSCs in a pellet or micromass culture system (Johnstone et al 1998;Jones et al 2002). The bioactive factors that stimulate chondrogenesis include TGF-β1, bone morphogenic protein, fibroblast growth factor, and insulin-like growth factor (Mastrogiacomo et al 2001). TGF-β1 acts via high-affinity interaction with a heteromeric receptor complex comprising two structurally-related serine-threonine kinases, the type I and II receptors, and transduces its signals through Smad proteins intracellularly (Miyazono 2000).…”

Section: Discussionmentioning

confidence: 99%

“…The marrow was drawn into syringes containing heparin (1,000 units) and kept on ice until later use. Bovine MSC were isolated by previously described methods (Worster et al 2000a(Worster et al , 2000bMastrogiacomo et al 2001;Holzer et al 2002;Bosnakovski et al 2004). Briefly, the bone marrow sample was washed twice with phosphate-buffered saline (PBS) and twice with Dulbecco's Modified Eagle Medium (DMEM; GIBCO BRL, Grand Islands, N.Y., USA).…”

Section: Harvesting and Isolation Of Bovine Mscmentioning

confidence: 99%

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Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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The bone marrow harbors a population of mesenchymal stem cells (MSCs) that possess the potential to differentiate into bone, cartilage, and fat, and along other tissue pathways. To date, MSCs from various species have been studied. Despite the bovine experimental model being widely used in experiments in vivo and in vitro, only a limited amount of information regarding bovine MSCs is available. The aim of this study was to isolate and induce the multilineage mesenchymal differentiation of bovine MSCs, thereby initiating further research on these cells. Bovine MSCs were isolated from eight calves, and osteogenic, chondrogenic, and adipogenic differentiation was induced by using a combination of previously reported protocols for other species. The level of differentiation was evaluated by histological examination and by analyzing the expression of tissue-specific genes by a quantitative "real time" reverse transcription/polymerase chain reaction technique. Following osteoinduction, the isolated fibroblast-like cells transformed into cuboidal cells and formed alkaline-phosphatase-positive colonies; during differentiation, these colonies transformed into mineralized nodules. In addition, osteogenesis was followed by osteocalcin and collagen type I mRNA expression. Chondrogenesis was confirmed by the demonstration of collagen type II, aggrecan, and sox9 mRNA expression in the cells stimulated by transforming growth factor β1 in monolayer culture. After being cultured in an adipogenesis-inducing medium, the MSCs responded by the accumulation of lipid vacuoles and the expression of adipocyte-specific genes. We have therefore demonstrated that cells harvested from bovine bone marrow are capable of in vitro extensive multiplication and multilineage differentiation, making them a relevant and invaluable model in the field of stem cell research.

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Section: Discussionmentioning

confidence: 99%

Section: Harvesting and Isolation Of Bovine Mscmentioning

confidence: 99%

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Bosnakovski¹,

et al. 2004

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“…A large number of signaling molecules that coordinate differentiation of MSCs into chondrocytes have been extensively investigated. Basic FGF was demonstrated to increase proliferation rate and life span in rabbit, dog and human MSC while maintaining their potential to differentiate towards fat, cartilage and bone [133,134]. The family of BMPs has a pivotal role in prechondrogenic condensations and the transition of chondroprogenitor cells into chondrocytes [135][136][137].…”

Section: Vivomentioning

confidence: 99%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

208

156

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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“…Therefore identifying expansion and differentiation conditions that promote a more chondrogenic phenotype is critical to enhancing their utility for cartilage tissue engineering applications. Differentiation conditions that have been shown to promote the chondrogenic potential of MSCs include a low oxygen (5%) microenvironment (Khan et al 2007;Buckley et al 2010a;Meyer et al 2010), various combinations of growth factors (Mastrogiacomo et al 2001;Sakimura et al 2006;Hennig et al 2007;Diekman et al 2010;Buxton et al 2011) and mechanical signals (Huang et al 2005;Mauck et al 2007;Huang et al 2010a;Huang et al 2010b;Kelly and Jacobs 2010;Li et al 2010; Thorpe et al 2010;Haugh et al 2011). …”

Section: Introductionmentioning

confidence: 99%

“…Augmenting MSC expansion conditions to enhance proliferation kinetics while maintaining multipotency is a critical obstacle to overcome in order to engineer mechanically functional tissues for clinical applications. Fibroblast growth factor-2 (FGF-2, also known as basic fibroblast growth factor) has been shown to be a potent stimulator during ex vivo expansion of both chondrocytes (Kato and Gospodarowicz 1985;Martin et al 1999;Martin et al 2001;Veilleux and Spector 2005) and MSCs (Banfi et al 2000;Mastrogiacomo et al 2001;Tsutsumi et al 2001;Bianchi et al 2003;Solchaga et al 2005;Khan et al 2008;Solchaga et al 2010), as well as regulating subsequent differentiation potential. Specifically, bone marrow derived MSCs cultured in the presence of FGF-2 have been shown to be physically smaller and proliferate more rapidly during monolayer expansion (Solchaga et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs

Buckley

Kelly

2012

Journal of the Mechanical Behavior of Biomedical Materials

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A bstractMSCs from non-cartilaginous knee joint tissues such as the infrapatellar fat pad (IFP) and synovium possess significant chondrogenic potential and provide a readily available and clinically feasible source of chondroprogenitor cells. Fibroblast growth factor-2 (FGF-2) has been shown to be a potent mitotic stimulator during ex vivo expansion of MSCs, as well as regulating their subsequent differentiation potential.The objective of this study was to investigate the longer term effects of FGF-2 expansion on the functional development of cartilaginous tissues engineered using MSCs derived from the IFP. IFP MSCs were isolated and expanded to passage 2 in a standard media formulation with or without FGF-2 (5ng/ml) supplementation.Expanded cells were encapsulated in agarose hydrogels, maintained in chondrogenic media for 42 days and analysed to determine their mechanical properties and biochemical composition. Culture media, collected at each feed, was also analysed for biochemical constituents.MSCs expanded in the presence of FGF-2 proliferated more rapidly, with higher cell yields and lower population doubling times. FGF-2 expanded MSCs generated the most mechanically functional tissue. Matrix accumulation was dramatically higher after 21 days for FGF-2 expanded MSCs, but decreased between day 21 and 42. By day 42, FGF-2 expanded MSCs had still accumulated ~1.4 fold higher sGAG and ~1.7 fold higher collagen compared to control groups. The total amount of sGAG synthesised (retained in hydrogels and released into the media) was ~ 2.4 fold higher for FGF-2 expanded MSCs, with only ~25% of the total amount generated being retained within the constructs. Further studies are required to investigate whether IFP derived MSCs have a diminished capacity to synthesise other matrix components important in the aggregation, assembly and retention of proteoglycans. In conclusion, expanding MSCs in the presence of FGF-2 rapidly accelerates chondrogenesis in 3D agarose cultures resulting in superior mechanical functionality.

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Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells

Cited by 137 publications

References 19 publications

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells

Cartilage tissue engineering for degenerative joint disease☆

Expansion in the presence of FGF-2 enhances the functional development of cartilaginous tissues engineered using infrapatellar fat pad derived MSCs

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