The present study was conducted during consecutive seasons of 2016 and 2017 seasons in a private nursery belonging to Sakha Horticulture Research Station at Kafr El-Sheikh Governorate, Egypt. This study aimed mainly to investigate the effect of growing media including different mixture of peat moss and sand as 1:1, 2:1 and 1:2 v/v and NPK fertilizer at rates of 0 , 1.5, 3 and 4.5 g/pot and their interaction on growth, flowering and chemical composition of cocks comb (Celosia argentea) plants. The results revealed that growing media containing peat + sand (2:1) had significant positive effect on number of leaves and branches/plant, number of inflorescences and chlorophyll content (SPAD) as well as leaf chemical composition parameters (total carbohydrate, N, P and K %) in both seasons. Applying the complete fertilizer of NPK at 4.5g/pot was the best treatment for vegetative growth, flowering and chemical constituents of leaves. The maximum beneficial effect on the vegetative growth characteristics, number of inflorescences/plant and chlorophyll content (SPAD) and total carbohydrate as well as leaf, N, P and K % were obtained for plants grown in in peat + sand (2: 1 v/v) and fertilized by NPK (20: 20: 20) at rate of 4.5 g/pot. It can be recommended that to produce high inflorescences with a good quality of Celosia argentea plants for different purposes in the gardens by planting them in the mixture of peat moss + sand at 2: 1 v/v and fertilizing with complete fertilizer NPK (20:20:20) at rate 4.5g/pot four times stating after one month from transplant and then 2 weeks intervals.