Effect of Different Doses of Vitamin E on the Incidence of Malformations in Pregnant Diabetic Rats

Viana, Marta; Castro, Mario; Barbas, Coral; Herrera, Emilio; Bonet, Bartolomé

doi:10.1159/000068907

Cited by 33 publications

(23 citation statements)

References 14 publications

(23 reference statements)

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“…Maternal diabetes entails embryo maldevelopment, leading to increased perinatal pathological situations, such as microsomias and congenital malformations (3,26), that are obvious from earlier stages of pregnancy according to our results and other previous works (10,49). Along these lines, we observed both an increased percentage of malformed embryos (4-fold increase on gestational day 13) and retarded growth in normal embryos from diabetic mothers.…”

Section: Discussionsupporting

confidence: 89%

Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period

Alcolea¹,

Lladó²,

García-Palmer³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

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Alcolea MP, Lladó I, García-Palmer FJ, Gianotti M. Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period. Am J Physiol Endocrinol Metab 293: E636-E644, 2007; doi:10.1152/ajpendo.00120.2007.-Mitochondria are cellular organelles that have been reported to be altered in diabetes, being closely related to its associated complications. Moreover, mitochondrial biogenesis and function are essential for proper embryo development throughout the placentation period, occurring during organogenesis, when a great rate of congenital malformations have been associated with diabetic pregnancy. Thus, the aim of the current work was to investigate the effect of the diabetic environment on mitochondrial function and biogenesis during the placentation period. For this purpose, we studied the oxidative phosphorylation system (OXPHOS) enzymatic activities as well as the expression of genes involved in the coordinated regulation of both mitochondrial and nuclear genome (PGC-1␣, NRF-1, NRF-2␣, mtSSB, and TFAM) and mitochondrial function (COX-IV, COX-I, and ␤-ATPase) in rat embryos from control and streptozotocininduced diabetic mothers. Our results reflected that diabetic pregnancy retarded and altered embryo growth. The embryos from diabetic mothers showing normal morphology presented a reduced content of proteins regulated through the PGC-1␣ mitochondriogenic pathway on gestational day 12. This fact was accompanied by several responses that entailed the activation of OXPHOS activities on the same day and the recovery of the content of the studied proteins to control levels on day 13. As a result, the mitochondria of these embryos would reach a situation close to control on day 13 that could allow them to follow the normal mitochondriogenic schedule throughout a gestational period in which the mitochondrial differentiation process is critical. Nevertheless, malformed embryos from diabetic mothers seemed to show a lower adaptation capability, which could exacerbate their maldevelopment.peroxisome proliferator-activated receptor-␥ coactivator-1␣; nuclear respiratory factors; mitochondrial transcription factor A; oxidative phosphorylation system THE ALTERED EMBRYO-FETAL DEVELOPMENT induced by the oxida-

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Section: Discussionsupporting

confidence: 89%

Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period

Alcolea¹,

Lladó²,

García-Palmer³

et al. 2007

American Journal of Physiology-Endocrinology and Metabolism

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“…Rats were randomly divided into two main groups: Control (n = 20) and experimental (n = 20) groups. Control group was further divided into negative control group (C1) which received distilled water, and positive control group (C2) that received VE (150 mg/kg/day) [13] and Se in the form of sodium selenite (Na 2 SeO 3 ) (150 µg/kg/day) for three months through intragastric tube [14]. This study was approved by the biomedical research ethics committee, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia.…”

Section: Methodsmentioning

confidence: 99%

Effect of vitamin E and selenium against aluminum-induced nephrotoxicity in pregnant rats

Abdel-Hamid

2014

Folia Histochem Cytobiol.

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Kidney is one of the most affected organs by aluminium toxicity. This study aimed to investigate the effect of aluminium chloride on the kidney of pregnant rats and to assess the efficiency of vitamin E and selenium in ameliorating this effect. Forty virgin albino rats were randomly divided into two main groups. Control rats were further divided into negative control group (C1, n = 10) which received distilled water and positive control group (C2, n = 10) that received vitamin E (VE, 150 mg/kg/day) and selenium (NaSe 150 µg/kg/day) for 3 months through intra-gastric tube. The experimental group was divided into an E1 subgroup in which rats received aluminium chloride (AlCl 3 , 150 mg/kg/day, n = 10) and E2 subgroup (n = 10) in which animals received the same dose of AlCl 3 plus VE and selenium at the same doses as C2 group for 3 months through intra-gastric tube. Conception of rats was allowed. AlCl 3 , VE and NaSe were given through intragastric tube during the whole length of the pregnancy, at the same doses as before pregnancy. At the 20th day of gestation dams were sacrificed, kidneys were dissected and processed for routine histological and immunohistochemical staining for identification of T-lymphocytes and macrophages. Integrated optical density of both cell types was assessed. AlCl3 administration induced histopathological changes in the kidney of pregnant rats and increased the density of CD3 and CD68 immunoreactive cells, suggestive of the associated aluminium-induced inflammatory process. Vitamin E and selenium minimized these harmful effects. The results suggest that diets rich in vitamin E and selenium and their supplements are advised particularly during pregnancy to alleviate the effects of possible excessive aluminium exposure.

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“…Pharmaceuticals, Athens, Greece) was aspirated with a size 21G needle, attached to an oral gavage, and administered orally at a dose of 500 mg/kg/ orally daily for 14 days (Viana et al, 2003).…”

Section: Preparation and Administration Of Alphatocopherol (Vitamin E)mentioning

confidence: 99%

Gamma radiation effects in the brain of rats: antioxidant and radioprotective properties of <i>Vernonia amygdalina</i> leaf extract

Owoeye¹,

Adesida²,

Onwuka³

et al. 2011

International Journal of Biological and Chemical Sciences

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Radiotherapy side effects have been attributed to free radical reactions. This study evaluated the antioxidant activity of the methanolic extract of V. amygdalina (MEVA) against gamma irradiation-induced oxidative stress in rats' brain. Seventy male rats weighing 130-160 g were randomized into seven groups, with each group comprising ten rats namely: control, MEVA at 250, and 500 mg/kg/day, radiation only, radiation plus MEVA at 250, and 500 mg/kg/day, and alpha-tocopherol (TOCO). Rats were treated for 14 days, irradiated on the 15 th day, and euthanized on the 16 th day. Bioassays of malondialdehyde for lipid peroxidation (LPO), reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) were carried out using standard techniques. Some of the brains were processed for paraffin wax embedment and stained with haematoxylin and eosin. Data were analyzed using ANOVA. Radiation caused a significant (p<0.01) elevation in LPO by about 142%. Pretreatment with 250 mg MEVA, 500 mg MEVA and TOCO reduced LPO significantly (p<0.01) by 56%, 65%, and 80% respectively, when compared with rats that received gamma radiation only. There was improvement in the histological alterations in the cerebellar layers and the Purkinje cell parameters of the pretreated groups when compared with the gamma-irradiated (R) group. MEVA demonstrated antioxidant and radioprotective effects in rats' brain.

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Effect of Different Doses of Vitamin E on the Incidence of Malformations in Pregnant Diabetic Rats

Cited by 33 publications

References 14 publications

Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period

Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period

Effect of vitamin E and selenium against aluminum-induced nephrotoxicity in pregnant rats

Gamma radiation effects in the brain of rats: antioxidant and radioprotective properties of <i>Vernonia amygdalina</i> leaf extract

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