Effect of different doses of hydrated C60 fullerene nanoparticles on ram semen during cool storage

Reduction of cryopreservation‐induced structural, functional and molecular damages in ram sperm by hydrated C60 fullerene

“…Therefore, a wide variety of antioxidants have been added to the extenders for many years to protect ram sperm against freeze-thawing damage (Bucak et al, 2013;Omur & Coyan, 2016), but desired success has still not been achieved. In this study, the effect of addition of C 60 HyFn, whose protective efficacy against loss of motility and viability, morphological disorder and LPO observed in ram sperm resulting from cryopreservation has been demonstrated (Güngör et al, 2021;Özer Kaya et al, 2021) imbalance in the oxidant-antioxidant system. Various fullerene derivatives have been claimed to reduce LPO and acrosomal damage and to increase antioxidant enzyme activities and motility in pig (at 4°C for 10 days, Li et al, 2019) and goat (at 32°C for 3 h, Murugan et al, 2002) semen stored for a short time.…”

“…Therefore, a wide variety of antioxidants have been added to the extenders for many years to protect ram sperm against freeze–thawing damage (Bucak et al., 2013; Omur & Coyan, 2016), but desired success has still not been achieved. In this study, the effect of addition of C 60 HyFn, whose protective efficacy against loss of motility and viability, morphological disorder and LPO observed in ram sperm resulting from cryopreservation has been demonstrated (Güngör et al., 2021; Özer Kaya et al., 2021), on cryopreservation‐induced motility, morphology and molecular damages was investigated and it was revealed that it has a protective feature in general.…”

“…It has been reported that motility, sperm velocity and linearity are associated with fertility, especially high values of VCL, VSL, VAP, LIN, STR, WOB and ALH have an important role in fertility success (Fernandez‐Lopez et al., 2022). C 60 HyFn supplementation has been suggested to prevent damages in motility, morphology, membrane functionality and viability in liquid‐stored (Özer Kaya et al., 2021) and frozen–thawed (Güngör et al., 2022) ram sperm by diminishing the imbalance in the oxidant–antioxidant system. Various fullerene derivatives have been claimed to reduce LPO and acrosomal damage and to increase antioxidant enzyme activities and motility in pig (at 4°C for 10 days, Li et al., 2019) and goat (at 32°C for 3 h, Murugan et al., 2002) semen stored for a short time.…”

“…(1995) produced hydrated C 60 fullerene, (C 60 HyFn), which is water soluble and not chemically modified. In in vivo and in vitro studies about the effects of C 60 HyFn having strong antioxidant activity (Gudkov et al., 2019) on the male reproductive system, it has been suggested that it reduces testicular damage induced by diabetes in rats (Bal et al., 2011) and prevents sperm morphological disorders and motility losses caused by semen cooling (5°C) (Özer Kaya et al., 2021) and freezing (−196°C) (Güngör et al., 2022) in rams. Yet, there is no scientific evidence about the effect of C 60 HyFn on sperm molecular damages caused by cryopreservation in rams.…”