Effect of Different Cryoprotectants and Sperm Densities of Orange Mud Crab, Scylla olivacea (Herbst, 1796) for Long-Term Storage of Spermatozoa

Ikhwanuddin, Mhd; Yin, Teng Phei; Menon, Abdul Jabbar; Jasmani, Safiah; Abol‐Munafi, Ambok Bolong

doi:10.17576/jsm-2015-4409-10

Cited by 5 publications

(6 citation statements)

References 16 publications

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Section: Lm and Tem Observationsupporting

confidence: 89%

“…The ndings of the sperm mass features in this study were similar to those described previously in S. olivacea (Noorbaiduri et al 2014;Ikhwanuddin et al 2015). The concentration in this study was slightly lower than that previous reported, which was 2.6-3.0 × 10 9 sperm/mL (Ikhwanuddin et al 2015). This is probably due to the difference in the method of sperm preparation, such as dilution factor, or the size and geographical difference of the crab samples.…”

Section: Lm and Tem Observationsupporting

confidence: 88%

“…The expulsed sperm mass was processed to isolate free sperm according to the method described earlier (Ikhwanuddin et al 2015). Brie y, the sperm mass was washed twice by mixing with 1-mL arti cial seawater (ASW) and centrifuged at 100×g for 10 min to precipitate tissue debris.…”

Section: Microscopic Examination Of the Expulsed Sperm Massmentioning

confidence: 99%

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Artificial Insemination of Mud Crab, Scylla olivacea

Foroutan,

kongsa,

Kanjanasopa

et al. 2024

Preprint

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Aiming at solving the problem of crablet production for mud crab commercial farming, an artificial insemination (AI) method in the mud crab, Scylla olivacea, was described. Placing a pair of cathodes to the inner coxa of both sides of the first pair of the pleopod and an anode to the inner abdominal flab upon the hind gut of sexually mature S. olivacea males and applying 5–6 V of DC current for 3–5 sec induced sperm mass expulsion. The sperm mass, comprising several spermatophores containing > 108 sperm/mL, was inserted into the vagina of the newly molted S. olivacea female. Out of 50 female trials, 10 of them developed ovarian maturation within 45 days, and 3 of these 10 crabs spawned. At full maturation of the ovary, needle aspiration of the ovarian tissue examined under light microscopy revealed approximately 50% mature oocytes with elevated fertilization membrane, suggesting successful fertilization. Histological and ultrastructural studies of the spermatheca revealed numerous sperm with acrosome reaction (AR) within its lumen, suggesting fertilization potential of the artificially inseminated sperm. Histologically, the mature ovary at 45 days following AI were composed of mature oocytes surrounded by numerous sperm with AR, suggesting sperm migration from the spermatheca into the ovarian tissue. This study reveals a high possibility of using AI for S. olivacea that could lead to the commercial supply of the crablets and a new insight into the internal fertilization of this economic species.

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Section: Lm and Tem Observationsupporting

confidence: 89%

Section: Lm and Tem Observationsupporting

confidence: 88%

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Artificial Insemination of Mud Crab, Scylla olivacea

Foroutan,

kongsa,

Kanjanasopa

et al. 2024

Preprint

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“…Photos of eggs were taken with a smartphone and the eggs were counted over photos. Fertilized eggs were separated based on the different coloring of unfertilized eggs (Ikhwanuddin et al 2015). The diameters of eggs were measured with a micrometer using a microscope.…”

Section: Reproductive Performancementioning

confidence: 99%

Effects of dietary soybean meal levels on reproduction parameters, the growth and gonad, gut, hepatopancreas histology of female African Cichlids Pseudotropheus socolofi

Pazar,

Bahadır Koca,

Özmen

2024

Journal of Limnology and Freshwater Fisheries Research

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This study was conducted to determine the effect of different soybean meal dietary supplementation levels on the growth, reproductive parameters, gonad, intestine and hepatopancreas histology of Pseudotropheus socolofi. Experimental groups were hand-fed to satiety with diets supplemented with soybean meal at levels of 3, 16, 27, 35, and 44% twice daily for 90 days. The results of this study showed that specific growth rate, weight gain, and survival rate were not affected by dietary soy proportions (P > 0.05). The worst FCR and final weight were found in those fed the diet containing 44% soy (P < 0.05). In female reproductive parameters, there was no significant difference between the groups in fertilization rate, egg production, hatching rate, egg diameter, gonadosomatic index and broodstock ovulation percentage (P > 0.05). However, due to pathological examinations in female individuals, a significant decrease was observed in the number of mature oocytes in the ovaries and goblet cells in the intestines with increasing soy levels in the diet (P < 0.05). As a result, using soybean meal up to 35% did not negatively affect growth. However, adding 44% soybean meal to diets caused histopathologically serious inflammatory reactions and decreased growth.

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“…High-quality diets decrease crab mortality, resulting in greater sperm production. Excess sperm can then be cryopreserved for future use in artificial insemination (Ikhwanuddin et al 2015c). In this study, we aimed to evaluate the effects of a squid (Loligo sp.)…”

Section: Introductionmentioning

confidence: 99%