Effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles

Rueda, Paloma; Fominaya, Jesús; Langeveld, J.P.M.; Bruschke, Christianne; Vela, Carmen; Casal, J. Ignacio

doi:10.1016/s0264-410x(00)00259-0

Cited by 76 publications

(53 citation statements)

References 21 publications

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“…The BV titer in preparations of PPV VLPs carrying the OVA 257-264 epitope (PPVOVA) was 5.2 ϫ 10 7 PFU/ml and that in standard control PPV VLPs preparations was 9.3 ϫ 10 7 PFU/ml. We detected no BVs by plaque assay in preparations treated with BEI or Triton X-100 or after purification by SEC, confirming previous results (9). These treatments were previously shown to have no effect on the physical integrity of PPV VLPs (9).…”

Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemsupporting

confidence: 90%

“…We have also recently shown that PPV VLPs are strong adjuvants when independently provided with Ag (14). Recombinant BVs have been detected in standard PPV VLP preparations, reaching titers greater than 10 7 PFU/ml (9). The data presented here may therefore suggest that the strong immunogenicity and adjuvant properties of PPV VLPs produced in the BV expression system are due to the adjuvant properties of BVs.…”

Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemsupporting

confidence: 54%

“…The LPS endotoxin content of the standard preparation was Ͻ0.01 U/ml. In some experiments, VLPs were purified by size-exclusion chromatography on a Sephacryl S-1000 SF column (Amersham Biosciences) (9). For BV inactivation, VLPs were treated with BEI and Triton X-100, as described above and in a previous study (9).…”

Section: Ppv Vlpsmentioning

confidence: 99%

“…Therefore, we used various procedures, such as size exclusion chromatography (SEC) to remove BVs from VLP preparations, or chemical treatment with BEI or Triton X-100 to inactivate them. SEC can easily remove BVs because BVs and PPV VLPs differ considerably in size (9). The BV titer in preparations of PPV VLPs carrying the OVA 257-264 epitope (PPVOVA) was 5.2 ϫ 10 7 PFU/ml and that in standard control PPV VLPs preparations was 9.3 ϫ 10 7 PFU/ml.…”

Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemmentioning

confidence: 99%

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Insect Baculoviruses Strongly Potentiate Adaptive Immune Responses by Inducing Type I IFN

Hervás-Stubbs

Rueda

López

et al. 2007

The Journal of Immunology

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155

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Baculoviruses (BVs) are dsDNA viruses that are pathogenic for insects. They have been used worldwide as selective bioinsecticides and for producing recombinant proteins in insect cells. Surprisingly, despite their widespread use in research and industry and their dissemination in the environment, the potential effects of these insect viruses on the immune responses of mammals remain totally unknown. We show in this study that BVs have strong adjuvant properties in mice, promoting potent humoral and CD8+ T cell adaptive responses against coadministered Ag. BVs also induce the in vivo maturation of dendritic cells and the production of inflammatory cytokines. We demonstrate that BVs play a major role in the strong immunogenicity of virus-like particles produced in the BV-insect cell expression system. The presence of even small numbers of BVs among the recombinant proteins produced in the BV expression system may therefore strengthen the immunological properties of these proteins. This adjuvant behavior of BVs is mediated primarily by IFN-αβ, although mechanisms independent of type I IFN signaling are also involved. This study demonstrates that nonpathogenic insect viruses may have a strong effect on the mammalian immune system.

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Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemsupporting

confidence: 90%

Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemsupporting

confidence: 54%

Section: Ppv Vlpsmentioning

confidence: 99%

Section: Ctl Priming By Ppv Vlps Produced In the Bv Expression Systemmentioning

confidence: 99%

See 2 more Smart Citations

Insect Baculoviruses Strongly Potentiate Adaptive Immune Responses by Inducing Type I IFN

Hervás-Stubbs

Rueda

López

et al. 2007

The Journal of Immunology

Self Cite

155

View full text Add to dashboard Cite

show abstract

“…It is difficult to separate baculovirus particles and expressed VLP completely. For clinical trials, chemical inactivation of contaminating baculovirusesin VLPs expressed and purified in baculovirus expression system has to be performed [55]. Otherwise baculovirus-free expression system is required to avoid baculovirus contamination.…”

Section: Insectsmentioning

confidence: 99%

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Kato¹,

Deo²,

Park

et al. 2012

J Biotechnol Biomaterial

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Virus-like particles (VLPs), which mimic the structure of authentic virus, are of significant importance owing to their wide ranging applications. VLPs have the potential to serve as candidate vaccine in addition to subunit vaccines and also serve as a nano-bioparticle scaffold for displaying complex foreign proteins. Here, we review different methods available to produce VLPs with various host expression systems including from microorganisms to mammalian cells and the current potential of silkworms as producers of these VLPs. Recently, silkworms have been used for efficient production of VLPs too in addition to mass production of recombinant proteins, which have contributed to molecular structural analysis, molecule-molecule interactions in biology and biotechnology.

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Viral Vaccines Purification

Kalbfuss

Reichl

2014

Vaccine Development and Manufacturing

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Effect of different baculovirus inactivation procedures on the integrity and immunogenicity of porcine parvovirus-like particles

Cited by 76 publications

References 21 publications

Insect Baculoviruses Strongly Potentiate Adaptive Immune Responses by Inducing Type I IFN

Insect Baculoviruses Strongly Potentiate Adaptive Immune Responses by Inducing Type I IFN

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Viral Vaccines Purification

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