Effect of different avian egg yolk types on fertilization ability of cryopreserved common carp (Cyprinus carpio) spermatozoa

Bozkurt, Yusuf; Yavaş, İlker; Yıldız, Cengiz

doi:10.1007/s10499-013-9728-4

Cited by 22 publications

(16 citation statements)

References 28 publications

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“…Furthermore, egg yolk contains cholesterol, fatty acids, and phospholipids. These compounds have been identified as protective agents (Watson 1976 cited by Bozkurt et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa

Muchlisin¹,

Nadiah²,

Nadiya³

et al. 2015

Czech J. Anim. Sci.

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Toxicity is a major limitation to successful spermatozoa cryopreservation of fish. Due to this problem, it is critical to find potential cryoprotectants which are more environmental-friendly, non-toxic, easily prepared, and available at affordable prices. Hence, the objective of the present study was to investigate several natural cryoprotectants for optimal cryopreservation of the African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa. Three natural cryoprotectants were tested -egg yolk, glucose, and honey, while DMSO was used as a control at different concentrations (5, 10, and 15%). Sperms were diluted with coconut water at a dilution level of 1 : 20 sperm to extender (v/v). Diluted sperms were kept at 4°C for 5 min, then at 0, -4, and -79°C for 5 min respectively, and stored in liquid nitrogen (-196°C) for 45 days. The cryopreserved sperms were thawed in a water bath (37°C) for 5 min and evaluated for fertilization and hatching rates. The data were subjected to analysis of variance (ANOVA), followed by comparison of means using Duncan's Multiple Range Test. The fertilization and hatching rates of African catfish in all cryoprotectants improved with concentration increasing from 5 to 10% but then decreased when concentration was increased to 15%. The ANOVA test showed that the differences in cryoprotectants used significantly affected fertilization and hatching rates of African catfish. Overall, the fertilization and hatching rates were higher in DMSO for all concentrations compared to other cryoprotectants. However, 10% egg yolk resulted in higher fertilization and hatching rates compared to other natural cryoprotectants. It was concluded that 10% egg yolk was the most suitable concentration for African catfish spermatozoa cryopreservation compared to other natural cryoprotectants tested.

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“…Furthermore, egg yolk contains cholesterol, fatty acids, and phospholipids. These compounds have been identified as protective agents (Watson 1976 cited by Bozkurt et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa

Muchlisin¹,

Nadiah²,

Nadiya³

et al. 2015

Czech J. Anim. Sci.

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“…There is a lack of available data regarding thawing conditions. Generally, thawing rates should be high to avoid recrystallization (Lahnsteiner, 2000;Bozkurt et al, 2014). According to the results of the present study, higher temperatures are necessary to recover membrane stability or spermatozoa.…”

Section: Discussionmentioning

confidence: 58%

Effect of Extender Compositions, Glycerol Levels, and Thawing Rates on Motility and Fertility of Cryopreserved Wild African Catfish (Clarias gariepinus) Sperm

Yavaş¹,

Bozkurt²

2017

Israeli Journal of Aquaculture - Bamidgeh

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The aim of this study was to determine the effect of different extenders, glycerol levels, and thawing rates on post-thaw sperm motility and fertilization ability of cryopreserved African catfish (Clarias gariepinus) sperm. Having determined the main spermatological properties (volume, motility, motility duration, spermatozoa concentration and pH), the pooled ejaculates were diluted with 3 different extenders containing different glycerol levels (5, 10 and 15%) respectively. Dilution ratio was 1:10 and the diluted sperm was packaged in 0.25 ml straws and left for 10 min equilibration at 4ºC. Following equilibration, the straws were exposed to liquid nitrogen vapor for 10 min and plunged into liquid nitrogen (-196ºC), and then exposed to different thawing rates (30ºC/20s and 40ºC/20s) to determine sperm motility and post-thaw motility duration. The highest post-thaw sperm motility, motility duration, and fertilization rate was 85%, 81s, and 95% respectively when sperm was frozen with the extender (ACSE 3) containing 15% glycerol (p<0.05). The protocol reported in this study can be successfully used for cryopreservation of African catfish sperm. The IJA appears exclusively as a peer-reviewed on-line open-access journal at http://www.siamb.org.il. To read papers free of charge, please register online at registration form. Sale of IJA papers is strictly forbidden.

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“…Permeating cryoprotectant will give protection from inside the cell such as DMSO, glycerol, DMF, etc. On the other hand, non-permeating will protect the cell from outside such as BSA, skim milk, egg yolk, sucrose, glucose, fructose, etc [3,4,5,6,7,8]. A combination of permeating and non-permeating cryoprotectants was expected to provide double protection for the cell during cryopreservation.…”

Section: Introductionmentioning

confidence: 99%

DMSO and sucrose combination as a cryoprotectant of Najawa carp (Cyprinus carpio L.) sperm cryopreservation

2020

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This study was designed to evaluate a DMSO (Dimethyl Sulfoxide) and sucrose combination as a cryoprotectant for cryopreservation of Najawa carp (Cyprinus carpio L.) sperm. The experiment was conducted according to the Completely Randomized Design (CRD) with four treatments 10% DMSO + 0.2 M sucrose, 10% DMSO + 0.1 M sucrose, 5% DMSO + 0.2 M sucrose, 5% DMSO + 0.1 M sucrose. Each treatment consisted of three replications. The parameter observed were sperm progressive motility of fresh sperm, diluted sperm before freezing, and diluted sperm after freezing 1 day; 7 days; 14 days; 21 days; 28 days. The data were analyzed by ANOVA. The result showed that there was no significant difference between treatments (Sig. > 0.05). The highest relative protection was 89.51% carried out by 5% DMSO + 0.1 M sucrose.

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Effect of different avian egg yolk types on fertilization ability of cryopreserved common carp (Cyprinus carpio) spermatozoa

Cited by 22 publications

References 28 publications

Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa

Exploration of natural cryoprotectants for cryopreservation of African catfish, Clarias gariepinus, Burchell 1822 (Pisces: Clariidae) spermatozoa

Effect of Extender Compositions, Glycerol Levels, and Thawing Rates on Motility and Fertility of Cryopreserved Wild African Catfish (Clarias gariepinus) Sperm

DMSO and sucrose combination as a cryoprotectant of Najawa carp (Cyprinus carpio L.) sperm cryopreservation

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