Effect of dietary α-linolenic acid on functional characteristic of Na+/K+-ATPase isoenzymes in whole brain membranes of weaned rats

Alteration of Na,K-ATPase isoenzymes in diabetic cardiomyopathy: effect of dietary supplementation with fish oil (n-3 fatty acids) in rats

“…The first is that although diabetes has been shown to be associated with an impairment in essential fatty acid metabolism [38][39][40], accurate information about changes in isoenzyme activity, subunit expression and the relative importance of the lipid environment in diabetes-induced sarcolemmal alterations is lacking. Second, previous dietary experiments demonstrated the dependence of the activity and ouabain affinity of isoenzymes on membrane fatty acid content [12,13], which influences membrane structure and function in brain. The third reason for our study is the clinical relevance of the experimental model and its utility in the evaluation of dietary influence.…”

“…The fact that both isoenzymes were affected could be due to an alteration in enzyme kinetics, protein subunit expression, or both. To answer this question, we assayed the pattern of isoenzyme expression and analysed the fatty acid content of cardiac membranes in parallel as previously described [12,13].…”

“…In previous work, we demonstrated that the properties of Na,K-ATPase isoenzymes in the rat brain could be modified by administration of dietary fatty acids [12] and, in particular, by fish oil supplementation [13]. These effects have been attributed to changes in the fatty acid content of plasma membranes.…”

“…Membrane environment can also be modified by diet [11][12][13] and a number of therapeutic diets are used [22]. Consumption of (n-3) polyunsaturated fatty acids, (e. g. fish oil concentrates) has been reported to lower the risk of cardiovascular disease [23,24].…”

An allometric comparison of microsomal membrane lipid composition and sodium pump molecular activity in the brain of mammals and birds

“…Changes in the fatty acid composition of neural membranes have been shown to alter the kinetic properties of the sodium pump (Gerbi et al, 1993(Gerbi et al, , 1994, and recently functional reconstitution experiments involving lipid crossovers between species, have provided direct evidence that membrane fatty acid composition is a major determinant of the molecular activity of the sodium pump enzyme (Else and Wu, 1999;Wu et al, 2004). Accordingly, we have analysed membrane lipid composition to determine whether variations in sodium pump molecular activity in the brain of the mammals and birds are underpinned by variations in membrane fatty acid composition.…”