Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary – An in vivo study

Grzesiak, Małgorzata; Kapusta, Klaudia; Kamińska, Kinga; Pałka, Sylwia; Kmiecik, Michał; Zubel-Łojek, Joanna

doi:10.1016/j.theriogenology.2021.06.001

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…t increase of nitric acid causes dilation of blood vessels and an increase in blood flow to the tissues. Nettle can reduce follicular atresia by increasing the blood flow to the ovaries and then increasing the penetration of oxygen to the granulosa cells [ 24 , 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice

Shamsi

Ganji

Mosayebi

et al. 2023

BMC Complement Med Ther

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Background One of the novel mechanisms in the pathogenesis of Polycystic ovary syndrome (PCOS) is low-grade chronic inflammation. Chamomile (Matricaria recutita L.) and Nettle (Urtica dioica), with phytoestrogenic and antioxidant properties, are traditionally used to treat gynecological diseases. This study investigated the immune-modulating effects of these two plants. Methods Following the induction of PCOS by subcutaneous injection (SC) of Dehydroepiandrosterone (DHEA) in BALB / C mice. Mice were treated in five groups: Sham, PCOS, PCOS + Chamomile, PCOS + Nettle, and PCOS + Chamomile and Nettle for 21 days. Ovarian morphology, blood antioxidant capacity, the abundance of Treg cells, and expression of matrix metalloproteinase-9 (MMP-9), transforming growth factor-ß (TGF-ß), cyclooxygenase-2 genes (COX-2), and tumor necrosis factor-alpha (TNF-α) were measured. Results Folliculogenesis, Cystic follicles, and corpus luteum improved in the treatment groups (P < 0. 05). Treg cells in the DHEA group were significantly reduced compared to the Sham group (P < 0. 01). However, this decrease was not corrected in treatment groups (P > 0. 05). Total serum antioxidant capacity was significantly increased in the treatment group of Nettle and Chamomile + Nettle (P < 0. 05). The expression of MMP9 and TGFβ genes in the PCOS group was significantly higher than the Sham group (P < 0. 05), which the expression of MMP9 was corrected by treatment with Chamomile + Nettle extract (P < 0. 05). Conclusion Chamomile and Nettle extract may be an effective supplement in improving the histological and immunological changes of PCOS. However, more research is needed to confirm its effectiveness in humans.

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Section: Discussionmentioning

confidence: 99%

Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice

Shamsi

Ganji

Mosayebi

et al. 2023

BMC Complement Med Ther

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“…In the F exposed group, measurements were over the limit for all animals for DHE, AMH while only 15 measures were yield for E2 and 12 for testosterone and progesterone. Serum levels of cholesterol (total and HDL cholesterol), triglycerides, insulin, glucose, aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT) were measured for n = 15 NE animals and n = 14 F animals using Beckman Coulter equipment similar to that used in a previous study [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

Folliculogenesis and steroidogenesis alterations after chronic exposure to a human-relevant mixture of environmental toxicants spare the ovarian reserve in the rabbit model

El Fouikar,

Van Acker,

Héliès

et al. 2024

J Ovarian Res

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Background Industrial progress has led to the omnipresence of chemicals in the environment of the general population, including reproductive-aged and pregnant women. The reproductive function of females is a well-known target of endocrine-disrupting chemicals. This function holds biological processes that are decisive for the fertility of women themselves and for the health of future generations. However, insufficient research has evaluated the risk of combined mixtures on this function. This study aimed to assess the direct impacts of a realistic exposure to eight combined environmental toxicants on the critical process of folliculogenesis. Methods Female rabbits were exposed daily and orally to either a mixture of eight environmental toxicants (F group) or the solvent mixture (NE group, control) from 2 to 19 weeks of age. The doses were computed from previous toxicokinetic data to reproduce steady-state serum concentrations in rabbits in the range of those encountered in pregnant women. Ovarian function was evaluated through macroscopic and histological analysis of the ovaries, serum hormonal assays and analysis of the expression of steroidogenic enzymes. Cellular dynamics in the ovary were further investigated with Ki67 staining and TUNEL assays. Results F rabbits grew similarly as NE rabbits but exhibited higher total and high-density lipoprotein (HDL) cholesterol levels in adulthood. They also presented a significantly elevated serum testosterone concentrations, while estradiol, progesterone, AMH and DHEA levels remained unaffected. The measurement of gonadotropins, androstenedione, pregnenolone and estrone levels yielded values below the limit of quantification. Among the 7 steroidogenic enzymes tested, an isolated higher expression of Cyp19a1 was measured in F rabbits ovaries. Those ovaries presented a significantly greater density/number of antral and atretic follicles and larger antral follicles without any changes in cellular proliferation or DNA fragmentation. No difference was found regarding the count of other follicle stages notably the primordial stage, the corpora lutea or AMH serum levels. Conclusion Folliculogenesis and steroidogenesis seem to be subtly altered by exposure to a human-like mixture of environmental toxicants. The antral follicle growth appears promoted by the mixture of chemicals both in their number and size, potentially explaining the increase in atretic antral follicles. Reassuringly, the ovarian reserve estimated through primordial follicles number/density and AMH is spared from any alteration. The consequences of these changes on fertility and progeny health have yet to be investigated.

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“…The levels of LH, FSH, and Estradiol were gradually elevated with increasing the concentrations of fenugreek due to the treatment of consequences induced by obesity through the reduction of free fatty acids that in turn enhance the regulation of gonadotropin expressions (Sharma et al, 2013). Moreover, the increase of estradiol levels in the experimental rats after the addition of fenugreek seeds can be explained by the increased abundance of crucial steroidogenic enzyme proteins in the rats' ovary caused by fenugreek supplementation, resulting in decreased CYP17A1 and increased CYP19A1 protein abundance, followed by the expected reduction in tissue testosterone concentration, elevated estradiol concentration, and thus increased plasma estradiol concentration (Grzesiak et al, 2021).…”

Section: Effect Of Fenugreek Powder On Gonadotropin Hormones (Lh Estr...mentioning

confidence: 99%

مسحوق الحلبة کعلاج واعد للعقم الناجم عن السمنة فی إناث الفئران البیضاء

صبح¹

2022

مجلة التربیة النوعیة و التکنولوجیا بحوث علمیة و تطبیقیة

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Infertility in women is a health problem that may sometimes be due to obesity This study aimed to estimate the influence of Fenugreek in different concentrations of obese induced infertile female albino rats. 28 and 7 white male albino rats were divided into 7 groups (5 rats for each group, 4 female and one male) weighing 220 ± 5g. The female rats in the first group were fed on a basal diet as a negative control; in the second group female rats were fed on a high-fat diet to induce obesity and were used as a positive control group. Females in fed on a high-fat diet with fenugreek powder. Bodyweight gain, feed intake, and feed efficiency ratio were calculated, lipid, liver and kidney functions, were determined. Hormones (LH, FSH, and Estradiol) were measured. The effect of fenugreek on the fertility of obese induced female rats was estimated by determining the number of fertile females after mating and pregnancy and the number of offspring. The results revealed a significant performance of fenugreek as a treatment of infertility induced by obesity as well as improvement of the health. Group 7 where the highest (7.5%) of fenugreek was used recorded the highest fertility, the highest LH, FSH, and estradiol levels 25.5 , 44 and 8.6 respectively In addition, the best lipid profile, liver, and kidney functions were obtained. This study demonstrated that fenugreek powder could be a promising approach to help Prevention infertility disorders induced by obesity .

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Effect of dietary supplementation with nettle or fenugreek on folliculogenesis and steroidogenesis in the rabbit ovary – An in vivo study

Cited by 5 publications

References 33 publications

Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice

Chamomile and Urtica dioica extracts improve immunological and histological alterations associated with polycystic ovarian syndrome in DHEA -induced mice

Folliculogenesis and steroidogenesis alterations after chronic exposure to a human-relevant mixture of environmental toxicants spare the ovarian reserve in the rabbit model

مسحوق الحلبة کعلاج واعد للعقم الناجم عن السمنة فی إناث الفئران البیضاء

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