Effect of dietary supplementation of bacteriophage on performance, egg quality and caecal bacterial populations in laying hens

Abstract

1. Bacteriophages (BP) have gained increasing attention as a treatment of bacterial infection for animals. However, the data pertaining to dietary application of BP for laying hens have been limited. 2. This study aimed to investigate the effect of dietary BP on laying performance, egg quality and caecal bacterial populations in laying hens. 3. The dietary BP used in this experiment was a mixture of individual BP targeting Salmonella gallinarum, Salmonella pullorum, Salmonella typhimurium, Salmonella enteritidis, Salmonella derby and Staphylococcus aureus. 4. A total of 360 Hy-Line Brown laying hens of 32 weeks of age were allotted to one of three dietary treatments with 6 replicates in a completely randomised design. The basal diet was prepared, and 0.4 or 0.8 g/kg BP mixture was supplemented to the basal diet. Diets were fed to hens for 8 weeks. 5. Laying performance and egg quality were not affected by dietary treatments. As inclusion levels of BP mixture in diets were increased, the DNA copy numbers for Salmonella spp. in the caecal contents decreased linearly, whereas the DNA copy numbers for Escherichia coli in the caecal contents increased linearly. 6. Results indicate that dietary supplementation of BP mixture decreases the target Salmonella spp. populations but increases Escherichia coli populations in the gastrointestinal tract of laying hens with little impact on laying performance and egg quality.

Effects of Clostridium butyricum and a Bacteriophage Cocktail on Growth Performance, Serum Biochemistry, Digestive Enzyme Activities, Intestinal Morphology, Immune Responses, and the Intestinal Microbiota in Rabbits

“…In this study, the data demonstrated that dietary CB supplementation slightly increased ADG and ADFI. Kim et al showed that a dietary BP cocktail decreased the F/G ratio and improved growth performance, which is consistent with the results of our feeding experiment [ 21 ]. The beneficial effects of BP treatment on growth performance are in agreement with other studies using BPs, which provide support for the use of BPs in rabbit diets.…”

Effects of bacteriophage supplement on the growth performance, microbial population, and PGC-1α and TLR4 gene expressions of broiler chickens

“…These results are, thus, in agreement with those reported by Kim et al, where body weight gain and FCR were improved by increasing the BP levels in the broilers’ diet 19 , 35 . A similar improvement in the layer's performance has also been observed in the previous studies 17 , 36 . However, Wang et al found that no the BP supplementation had no beneficial effect on the body weight and FCR of broiler chickens 37 .…”

“…A similar improvement in the layer's performance has also been observed in the previous studies 17,36 . However, Wang et al found that no the BP supplementation had no beneficial effect on the body weight and FCR of broiler chickens 37 .…”

Productive performance of weanling piglets was improved by administration of a mixture of bacteriophages, targeted to control Coliforms and Clostridium spp. shedding in a challenging environment

“…Several investigators have examined the positive effect of bacteriophage on the proliferation of coliforms (Carvalho et al., ; Cha et al., ), C. perfringens (Miller et al., ; Kim et al., ), Staphylococcus aureus (Obeso et al., ) and Salmonella spp. (Gebru et al., ; Kim et al., ).…”