Effect of dietary supplementation of organic zinc and copper on in vitro semen fertility in goat

Hemalatha, K.; Arunachalam, A.; Selvaraju, Sellappan; Krishnaiah, M. Venkata; Rani, G. Pushpa; Mishra, Ashish; Soren, N. M.; Reddy, I. J.; Ravindra, J.P.

doi:10.1016/j.smallrumres.2018.02.003

Cited by 15 publications

(4 citation statements)

References 28 publications

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“…Recently, Manohar et al (2018) reported that organic zinc and copper supplementation have antioxidant protective effect and it enhances sperm functional characteristics in goats. There was a pronounced influence of trace minerals on the cleavage rate, sperm-zonapellucida binding ability and the fertility related properties in goats (Hemalatha et al 2018). An augmentation in the serum Cu and Zn levels can be achieved by their dietary supplementation which ultimately translates into improvement in overall hormone production, semen quality at different stages such as fresh semen, equilibrated and post thawed semen (Arangasamy et al 2018a, b).…”

Section: Resultsmentioning

confidence: 99%

“…induced cryoinjuries and/or cryo damages to sperm cells. Hemalatha et al (2018) reported enhancement in in vitro fertilizing capacity of frozen semen after dietary supplementation of organic zinc and copper to the male goats. A significantly higher zona pellucida binding capacity of sperm and cleavage rate percent were observed in mineral treated groups as compared to control group.…”

Section: Resultsmentioning

confidence: 99%

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Effect of azolla supplementation in feed on semen freezability in bucks

Gangwar¹,

Kumar²,

Kharche³

et al. 2019

Indian J of Anim Sci

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The present study was conducted to evaluate the effect of azolla supplementation on semen freezability in Barbari bucks. Ten adult Barbari bucks (2â€“4 years old) were selected, and divided into control and treatment group (5 bucks in each group) as per completely randomized design. Bucks of control group were fed with 400 g concentrate pellet/day along with 6â€“7 h grazing while bucks of treatment group were supplemented with 100 g fresh azolla along with 400 g concentrate pellet and 6â€“7 h grazing. Semen ejaculates (8) were collected from each buck of control and treatment groups after 60 days of azolla supplementation. Immediately after collection, semen samples were evaluated for colour, volume, density, mass motility, progressive motility, live sperm count, acrosomal integrity and hypo-osmotic swelling positive spermatozoa. Then semen samples were diluted with trisâ€“citric acid fructose diluents having 6% (v/v) glycerol as cryoprotective agent and 10% (v/v) egg yolk. After equilibration, semen samples were filled in straws and vapour frozen for 10 min 4 cm above the liquid nitrogen and finally stored into liquid nitrogen container. Post thaw motility, live sperm count, abnormalities, acrosomal integrity and hypo-osmotic swelling test were conducted to check the effect of azolla feeding on the freezability. Post thaw motility, live sperm count, acrosomal integrity and hypo-osmotic swelling positive spermatozoa were significantly higher in treatment group. It could be concluded that dietary azolla supplementation to breeding bucks improved the quality and freezability of semen favouring the use in artificial insemination program.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effect of azolla supplementation in feed on semen freezability in bucks

Gangwar¹,

Kumar²,

Kharche³

et al. 2019

Indian J of Anim Sci

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“…In another study comparing the bioavailability of zinc from zinc-glycine (Zn-Gly) and zinc-methionine (Zn-Met) with zinc sulfate (ZnSO 4 ), it was found that zinc in Zn-Met had higher bioavailability than Zn-Gly [39]. Organic zinc supplementation increased semen volume, sperm concentration, and improved the spermatozoal membrane integrity in bulls and bucks [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes

Slyvchuk,

Shtapenko,

Yaremchuk

et al. 2023

Innov Biosyst Bioeng

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Background. Zinc is essential for male reproductive function as it is necessary for spermatozoa maturation, capacitation, acrosome reaction, and fertilization. It has been established that the use of chelates compounds of metals with amino acids, nucleotides, peptides, and carbohydrates is not only more effective but also economically justified. Objective. The aim of this study was to evaluate and compare the effect of different concentrations of Zn glutamic amino acid chelate (ZnGlu) on boar spermatozoa motility, viability parameters, and prooxidant-antioxidant homeostasis during in vitro incubation. Methods. Freshly ejaculated boar semen, after the addition of "Ecosperm" diluent, was divided into groups: a control group and three experimental groups. Zinc glutamate was supplemented to the experimental samples at concentrations of 1.0, 2.0, and 5.0 µg/ml, respectively. Semen samples were stored at 18 °C for 4 days. Every 24 hours, sperm motility and viability and the antioxidant status were assessed by the level of diene conjugates (DC), concentration of malondialdehydere (MDA), and the activities of catalase and superoxide dismutase. Results. It was observed that boar sperm motility decreased with the extension of storage time, while the addition of 2.0 and 5.0 µg ZnGlu significantly improved sperm total motility and the percentage of vitality spermatozoa during 48–96 hours of incubation. Our studies also demonstrate that ZnGlu possess a protective effect in alleviating oxidative stress in boar sperm in vitro. The addition of ZnGlu significantly reduced the content of MDA and DC in ejaculate samples in all experimental groups during incubation, compared to the control group. Moreover, the activity of superoxide dismutase and catalase increased after adding ZnGlu to the boar semen, especially at concentrations of 2.0 and 5.0 mg/ml (P < 0.05). Conclusions. These data demonstrate that the supplementation of zinc glutamate enhances the antioxidant defence system of sperm and improves quality of boar semen in vitro.

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“…Various in vivo or in vitro approaches have been made for the past few decades to retain spermatozoa motility at maximum level. The in vivo method, consists of supplementation of mineral, proteins, high energy diet to male animals and enhancement of semen characteristic prior to collection of semen samples (Arunachalam et al 2018;Hemalatha et al 2018;Narasimhaiah et al 2018). In the in vitro method, various types of semen additives are included during the dilution and equilibration period and prior to cryopreservation process (Dance et al 2016, Mayasula et al 2022.…”

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confidence: 99%

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

Arunachalam

BACKIALAKSHMI

SHARANYA

et al. 2022

Indian J of Anim Sci

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The great expectation of the dairy owners from the frozen semen industry is quality semen with high fertility. Many researchers concentrate in this area to produce frozen semen of high quality and fertility. However, the problem still exists to minimize or reduce the cryoinjury related damages that occur during the process of cryopreservation with the use of all kinds of semen additives. The success rate of the additives added semen is variable at a larger scale. Therefore, any new molecules which can effectively prevent or minimize the cryoinjury are warranted and it could be low cost substances. Our incidental finding and protein identification with molecular weight ≈ 19 kDa (cold-inducible RNA-binding protein CIRBP) in goat spermatozoa seems to be effective in minimizing cryoinjury. The identified CIRBP was confirmed via Western blotting. In future, further studies are needed to confirm the molecular mechanism of the CIRBP on the cryoinjury as semen additives, and application of this protein added semen in the field to improve the level of conception rate in cattle.

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Effect of dietary supplementation of organic zinc and copper on in vitro semen fertility in goat

Cited by 15 publications

References 28 publications

Effect of azolla supplementation in feed on semen freezability in bucks

Effect of azolla supplementation in feed on semen freezability in bucks

Effect of Zinc Supplementation to Diluent Boar Semen on Sperm Characteristics and Activity of Antioxidant Enzymes

Novel Cold-Inducible RNA-Binding Protein (CIRBP) in goat spermatozoa: A possible cryo-stress reliever during the process of Cryopreservation

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