This study was planned to determine the effects of sumac juice and different application methods on the fattening and immune performance of broiler chickens. The study was started with 240 Ross 308 broiler chickens at the age of 1 day. The trial model consisted of 6 groups in total, in the 3*2 interaction model, in which three different sumac levels were given in two different ways. Each group was arranged to contain 4 replications, and 10 broilers per replication. Birds were supplemented with three different levels (0, 5, and 10 g/liter) of sumac both in drinking water and via inhalation. This duration of experiment was 42 days. Live weight and feed consumption were determined on the 10th, 24th and 42nd days. On the 32nd day of the study, the chickens were vaccinated against Infectious bronchitis virus (IBV) and Newcastle virus (NDV) by adding them to drinking water for immunity test. During slaughter, blood samples were taken for antibody titers and hematological tests and lipid profile were performed. It was observed that the additive of sumac given by drinking water or inhalation did not have a significant effect on the growth performance of broilers. However, steam application significantly reduced feed consumption and body weight gain. Sumac was not effective in reducing the lipid profile particularly cholesterol in the serum of broiler chickens. In addition, although the production of antibodies against NDV and IBV of broilers was improved to some extent, the antibody titer against IBV showed a significantly positive improvement with inhalation administration