Effect of Dietary-Resistant Starch on Inhibition of Colonic Preneoplasia andWntSignaling in Azoxymethane-Induced Rodent Models

Abstract

Dietary fiber has been reported to prevent preneoplastic colon lesions. The aim of this study was to determine the effect of resistant starches, novel dietary fibers, on the development of colonic preneoplasia and Wnt signaling in azoxymethane (AOM)-treated rats and mice fed resistant starches at 55% of the diet after AOM treatment. Another objective was to determine the effect of resistant starches on the development of preneoplasia in rats treated with antibiotics (Ab), administered between AOM treatment and resistant starch feeding. Diets containing resistant starches, high-amylose (HA7), high-amylose-octenyl succinic anhydride (OS-HA7), or high-amylose-stearic acid (SA-HA7) were compared with control cornstarch (CS). The resistant starch content of the diets did not alter the yield of colonic lesions but animals treated with AOM and fed the diet with the highest resistant starch content, SA-HA7 developed the highest average aberrant crypt foci (ACF) per animal. Mice fed the OS-HA7 diet had decreased expression of some upstream Wnt genes in the colonic crypts. This study suggests that further research is needed to determine if resistant starch impacts colon carcinogenesis in rodents.

Determination of short-chain fatty acids as putative biomarkers of cancer diseases by modern analytical strategies and tools: a review

“…A similar positive effect was also observed in mice with adenoma and adenocarcinoma undergoing the same treatment (69). The vast majority of the studies suggest that butyrate is pivotal for maintaining colon health and CRC suppression through dietary fiber (40,(70)(71)(72). On the other hand, the cellular effects of butyrate are complex, and therefore not only positive but also negative influences on cell proliferation, differentiation, and apoptosis were observed (73).…”