Effect of dietary protein supply originating from soybean meal or casein on the intestinal microbiota of piglets

Rist, V. T. S.; Weiss, Eva; Sauer, N.; Mosenthin, R.; Eklund, M.

doi:10.1016/j.anaerobe.2013.10.003

Cited by 71 publications

(72 citation statements)

References 60 publications

(57 reference statements)

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“…and Bifidobacterium spp. in ileal digesta of HP treatments are in agreement with a recent study by Rist et al [17], where piglets fed high dietary CP levels showed an increased growth and proliferation of lactic-acid bacteria in ileal digesta. As content of soybean meal in the present study was greater in HP than in LP diets, enhanced availability of fermentable carbohydrates in the small intestine can be suggested, thereby stimulating ileal growth of lactobacilli and bifidobacteria [17].…”

Section: Discussionsupporting

confidence: 92%

“…in ileal digesta of HP treatments are in agreement with a recent study by Rist et al [17], where piglets fed high dietary CP levels showed an increased growth and proliferation of lactic-acid bacteria in ileal digesta. As content of soybean meal in the present study was greater in HP than in LP diets, enhanced availability of fermentable carbohydrates in the small intestine can be suggested, thereby stimulating ileal growth of lactobacilli and bifidobacteria [17]. Furthermore, HP diets could increase the availability of free AA in the small intestine [17], contributing much more preformed AA of dietary and endogenous origin to bacterial growth in the upper part of the digestive tract than microbial de novo synthesis of AA [13].…”

Section: Discussionsupporting

confidence: 92%

“…As content of soybean meal in the present study was greater in HP than in LP diets, enhanced availability of fermentable carbohydrates in the small intestine can be suggested, thereby stimulating ileal growth of lactobacilli and bifidobacteria [17]. Furthermore, HP diets could increase the availability of free AA in the small intestine [17], contributing much more preformed AA of dietary and endogenous origin to bacterial growth in the upper part of the digestive tract than microbial de novo synthesis of AA [13]. Furthermore, analysis of overall microbiota composition in ileal digesta by amplicon sequencing supported an increasing effect on Lactobacillus proportion upon feeding of HP + diets.…”

Section: Discussionmentioning

confidence: 97%

“…and Bifidobacterium spp. in the GIT has been reported to be beneficial for the host animal [17] due to their ability for bacteriocin production [59]. Moreover, proliferation of pathogenic bacteria may be inhibited through the production of short-chain fatty acids (SCFA) and lactic acid, being associated with a lower pH, causing a hostile environment for some acid-sensitive bacteria strains [60].…”

Section: Discussionmentioning

confidence: 99%

“…Thus, excessive nitrogen (N) excretion by pigs is mitigated, resulting in a decrease of environmental pollutants [15, 16]. In contrast, increasing the dietary CP intake may stimulate the proliferation of almost all bacteria groups over the entire GIT including beneficial bacteria, such as Bifidobacterium spp., and potentially pathogenic bacteria, such as Bacteroides groups [17]. Furthermore, there is increasing evidence that interactions of supplemental probiotics with dietary CP level affect the intestinal microbiome at the ileal level [18].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs

Kaewtapee

Burbach

Tomforde

et al. 2017

J Animal Sci Biotechnol

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Background Bacillus spp. seem to be an alternative to antimicrobial growth promoters for improving animals’ health and performance. However, there is little information on the effect of Bacillus spp. in combination with different dietary crude protein (CP) levels on the ileal digestibility and microbiota composition. Therefore, the objective of this study was to determine the effect of Bacillus spp. supplementation to low- (LP) and high-protein diets (HP) on ileal CP and amino acid (AA) digestibility and intestinal microbiota composition.MethodsEight ileally cannulated pigs with an initial body weight of 28.5 kg were randomly allocated to a row-column design with 8 pigs and 3 periods of 16 d each. The assay diets were based on wheat-barley-soybean meal with two protein levels: LP (14% CP, as-fed) and HP diet (18% CP, as-fed). The LP and HP diets were supplemented with or without Bacillus spp. at a level of 0.04% (as-fed). The apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of CP and AA was determined. Bacterial community composition from ileal digesta was analyzed by Illumina amplicon sequencing and quantitative real-time PCR. Data were analyzed as a 2 × 2 factorial design using the GLIMMIX procedures of SAS.ResultsThe supplementation with Bacillus spp. did not affect both AID and SID of CP and AA in growing pigs. Moreover, there was no difference in AID of CP and AA between HP and LP diets, but SID of cystine, glutamic acid, glycine, and proline was lower (P < 0.05) in pigs fed the HP diets. The HP diets increased abundance of Bifidobacterium spp. and Lactobacillus spp., (P < 0.05) and by amplicon sequencing the latter was identified as predominant genus in microbiota from HP with Bacillus spp., whereas dietary supplementation of Bacillus spp. increased (P < 0.05) abundance of Roseburia spp..ConclusionsThe HP diet increased abundance of Lactobacillus spp. and Bifidobacterium spp.. The supplementation of Bacillus spp. resulted in a higher abundance of healthy gut associated bacteria without affecting ileal CP and AA digestibility, whereas LP diet may reduce the flow of undigested protein to the large intestine of pigs.

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Section: Discussionsupporting

confidence: 92%