Effect of dietary protease supplementation on growth performance, water quality, blood parameters and intestinal morphology of Nile tilapia (<i>Oreochromis</i><i>niloticus</i>)

Saleh, Elham S. E.; Tawfeek, Samar S.; Abdel-Fadeel, Asmaa A. A.; Abdel-Daim, Asmaa S. A.; Abdel‐Razik, Abdel‐Razik H.; Youssef, Ibrahim

doi:10.1111/jpn.13591

Cited by 13 publications

(18 citation statements)

References 32 publications

(59 reference statements)

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“…In current study, total protein and albumin remained unaffected by protease supplementation. Similar to our results, no significant differences in glucose, albumin and total protein content were reported for protease supplementation in gibel carp (Liu et al, 2018) and tilapia (Saleh et al, 2021). These findings confirmed that optimum protease supplementation did not induce stress on fish physiology.…”

Section: Biochemical Parameterssupporting

confidence: 91%

“…Similarly, Hassan et al (2019) reported that dietary protease supplementation improved RBCs count in tilapia. Contrarily, no significant difference in RBCs count and Hb level was observed in tilapia and gibel carp in response to protease supplemented diets (Saleh et al, 2021; Liu et al, 2018). Besides this, no significant difference in WBCs was observed among all treatments in this study.…”

Section: Discussionmentioning

confidence: 98%

“…It could be ascribed to the scarcity of endogenous digestive enzymes in juvenile and reduced palatability due to the presence of connective tissues, feathers, skin, high ash and fibre content in PBM (Fowler, 1991; Srour et al, 2016; Tabinda & Butt, 2012). Studies regarding the augmentation of growth performance in response to exogenous protease supplementation have been reported in tilapia (Hassaan et al, 2019; Hassaan et al, 2020; Huan et al, 2018; Kamel et al, 2015; Li et al, 2019; Saleh et al, 2021; Shi et al, 2016; Wu et al, 2020), African catfish (Kemigabo et al, 2019), rainbow trout (Dalsgaard et al, 2012; Drew et al, 2005b; Yigit et al, 2018), European sea bass (Goda et al, 2020), black carp (Chen et al, 2009) and gibel carp (Liu et al, 2018; Shi et al, 2016). This improvement in growth performance could be attributed to better protein utilization due to high proteolytic activity of protease in complex protein (Hassaan et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Section: Growth Performance and Feed Utilizationmentioning

confidence: 99%

“…Previous studies revealed that exogenous dietary protease supplementation improved the feed conversion efficiency (FCR), nutrient digestibility and growth in fish (Cowieson & Ravindran, 2008; Hassaan et al, 2020; Saleh et al, 2021; Song et al, 2017) while it reduced the nitrogen emission in different fish including gibel carp, Carassius gibelio (Yang et al, 2004), black sea bass, Centropristis striata (Dawson et al, 2018) and tilapia, Oreochromis niloticus (Li et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The effectiveness of protease supplemented poultry by‐product meal‐based diet on growth, nutrient digestibility and digestive enzyme activities of rohu ( Labeo rohita )

Maryam

Shah

Fatima

et al. 2022

Aquaculture Research

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Proteases are the enzymes responsible for the hydrolysis of complex proteins into simpler units for better digestion and absorption of nutrients. This study was conducted to determine the optimum supplementation level and effects of dietary protease addition in poultry by‐product meal‐based diet (PBM) on growth performance, nutrient digestibility, digestive enzyme activities and physiochemical parameters of juvenile rohu, Labeo rohita (initial weight: 11.33 ± 0.02 g). For this purpose, seven iso‐nitrogenous and iso‐lipidic experimental diets were formulated to contain a positive control (PC) diet (containing fishmeal and no protease), a negative control (NC) diet (containing PBM and no protease) and five other PBM‐based diets supplemented with exogenous protease at the levels of 150, 300, 450, 600 and 750 mg/kg diet. Results indicated that growth performance and body indices of fish fed protease supplemented diets were higher (p < 0.05) compared with those fed the NC diet, while maximum increase was recorded at 450 mg/kg protease supplementation level, which was similar to the PC diet (p > 0.05). Protease supplementation up to 450 mg/kg level linearly improved (p < 0.05) the whole‐body proximate composition and digestibility of dry matter (DM), crude protein (CP), crude lipid (CL) and crude ash (CA) in rohu juveniles. A similar trend (p < 0.05) was observed in the activities of amylases, lipases and proteases in intestinal and hepatopancreatic tissues, peaking at 450 mg/kg which was also similar (p > 0.05) to the PC diet. Protease supplementation significantly improved (p < 0.05) haemoglobin (Hb), haematocrit (Htc) and red blood cells (RBCs) of juveniles while, white blood cells (WBCs) and serum indices remained unaffected (p > 0.05). Based on quadratic regression analysis, the optimal dietary protease supplementation in PBM‐based diet for rohu was 433 mg/kg. Conclusively, increase in protease supplementation from 0 to 450 mg/kg levels improved the fish performance and decreased thereafter with further increase in protease levels (600–750 mg/kg).

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Section: Biochemical Parameterssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Growth Performance and Feed Utilizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effectiveness of protease supplemented poultry by‐product meal‐based diet on growth, nutrient digestibility and digestive enzyme activities of rohu ( Labeo rohita )

Maryam

Shah

Fatima

et al. 2022

Aquaculture Research

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Replacing starch with resistant starch (Laminaria japonica) improves water quality, nitrogen and phosphorus budget and microbial community in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)

Wang

Zuo

Wang

et al. 2023

Water Environment Research

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It is essential to increase the use of carbohydrates as an energy source and improve protein synthesis and utilization to reduce ammonia nitrogen emissions. A 60‐day cultural experiment was conducted to assess the impact of resistant starch (kelp meal, Laminaria japonica) replacing starch on water quality, nitrogen and phosphorus budget and microbial community of hybrid snakehead. Approximately 1350 experimental fish (11.4 ± 0.15 g) were randomly divided into control group (C, 20% starch) and four resistant starch groups: low replacement group (LR, 15% starch), medium replacement group (MR, 10% starch), high replacement group (HR, 5% starch) and full replacement group (FR, 0% starch). The crude protein and crude fat content of hybrid snakehead fish fed with the FR diet had the most significant improvement (P < 0.05). However, resistant starch also increased the effectiveness of nitrogen and phosphorus utilization in hybrid snakeheads, which decreased the proportion of total nitrogen and total phosphorus in tail water. The minimum nitrogen and phosphorus emission rate was when the starch level was 6.1%. Denitrifying microbes including Gemmobacter, Rhodobacter, Emticicia and Bosea have become much more prevalent in group FR (P < 0.05). In general, replacing starch with resistant starch can enhance the rate at which nitrogen and phosphorus are used in feeding, lessening water pollution and altering environmental microbial composition. Practitioner Points Resistant starch (RS) improves whole fish nutritional content. Resistant starch improves dietary nitrogen and phosphorus utilization. Resistant starch acts as a carbon source and encourages the colonization of denitrifying bacteria in water.

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Effect of dietary phytase and protease supplementation on the growth performance and apparent nutrient digestibility in juvenile Pacific white shrimp (Litopenaeus vannamei) fed fish meal–free and phosphorus limiting diets

Coelho,

Tacon,

Lemos

2024

Aquacult Int

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This study investigated the effects of exogenous enzyme supplementation, speci cally phytase and protease, in sh meal-free and phosphorus-limited diets for juvenile Litopenaeus vannamei through two feeding trials The trials aimed to assess shrimp growth performance and apparent nutrient digestibility simultaneously in a clear-water recirculating tank system (34 ppt, 30°C) employing a continuous feeding regime, with feces being collected on a daily basis throughout the feeding trials. In the rst feeding 50day feeding trial shrimp (3.4 g initial body weight) were fed diets supplemented with phytase (1000 and 2000 FTU/kg) and phytase together with protease (1000 FTU/kg + protease and 2000 FTU/kg + protease), in addition to animals fed a positive control (supplemented inorganic phosphate) and a negative control diet without supplementation. In the second shrimp feeding trial (4.3 g initial body weight), in addition to negative and positive controls, shrimp were fed increasing levels of phytase (1000, 2000, 3000, 4000 and 8000 FTU/kg) over a 42-day experimental period. Both feeding trials showed bene cial effects phytase addition compared to the negative control, with signi cant improvements (P < 0.05) observed at dietary phytase levels of 2000 FTU/kg and above. Gains were obtained in growth performance (observed weekly growth of 1.46 and 1.86 g/week for shrimp fed the negative control and diet supplemented with 3000 FTU/kg, respectively), and apparent phosphorus digestibility increasing from 41.7% in animals fed the negative control diet to 52.9% in animals fed the 3000 FTU/kg supplemented. Results indicated that phytase supplementation yielded signi cant improvements in shrimp growth performance and phosphorus digestibility compared to the negative control. Notably, the observed bene ts were evident at speci c dietary phytase levels. However, the addition of protease supplements did not demonstrate any discernible effects on shrimp performance under the experimental conditions. Overall, these ndings underscore the potential of phytase supplementation as a means to enhance nutrient utilization and promote optimal growth in Litopenaeus vannamei. Further investigations are warranted to explore the full range of bene ts and mechanisms associated with protease supplementation in shrimp diets.

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Effect of dietary protease supplementation on growth performance, water quality, blood parameters and intestinal morphology of Nile tilapia (Oreochromisniloticus)

Cited by 13 publications

References 32 publications

The effectiveness of protease supplemented poultry by‐product meal‐based diet on growth, nutrient digestibility and digestive enzyme activities of rohu ( Labeo rohita )

The effectiveness of protease supplemented poultry by‐product meal‐based diet on growth, nutrient digestibility and digestive enzyme activities of rohu ( Labeo rohita )

Replacing starch with resistant starch (Laminaria japonica) improves water quality, nitrogen and phosphorus budget and microbial community in hybrid snakehead (Channa maculata ♀ × Channa argus ♂)

Effect of dietary phytase and protease supplementation on the growth performance and apparent nutrient digestibility in juvenile Pacific white shrimp (Litopenaeus vannamei) fed fish meal–free and phosphorus limiting diets

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