Effect of dietary polyunsaturated fatty acids on uterine prostaglandin synthesis in the cow

Cheng, Zhangrui; Robinson, Robert; Pushpakumara, P. G. A.; Mansbridge, R J; Wathes, D C

doi:10.1677/joe.0.1710463

Cited by 75 publications

(45 citation statements)

References 67 publications

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“…The concentrations of PGE 2 and PGF 2α in the spent medium were measured with charcoal-dextran-coated RIA methods described previously (Cheng et al 2001). The PG antisera were a kind gift from Dr N L Poyser (University of Edinburgh, Edinburgh, UK).…”

Section: Quantification Of Pgs Using Radioimmunoassay (Ria)mentioning

confidence: 99%

BVDV alters uterine prostaglandin production during pregnancy recognition in cows

Cheng¹,

Abudureyimu²,

Oguejiofor³

et al. 2016

Reproduction

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Embryonic mortality in cows is at least in part caused by failure of pregnancy recognition (PR). Evidence has shown that bovine viral diarrhoea virus (BVDV) infection can disrupt pregnancy. Prostaglandins (PG) play important roles in many reproductive processes, such as implantation. The aim of this study was to investigate the effect of BVDV infection on uterine PG production and PR using an in vitro PR model. Bovine uterine endometrial cells isolated from ten BVDV-free cows were cultured and treated with 0 or 100 ng/mL interferon-τ (IFNT) in the absence or presence of non-cytopathic BVDV (ncpBVDV). PGF 2α and PGE 2 concentrations in the spent medium were measured using radioimmunoassays, and in the treated cells expression of the genes associated with PG production and signalling was quantified using qPCR. The results showed that the IFNT challenge significantly stimulated PTGS1 and PTGER3 mRNA expression and PGE 2 production; however, these stimulatory effects were neutralised in the presence of ncpBVDV infection. ncpBVDV infection significantly increased PTGS1 and mPGES1 mRNA expression and decreased AKR1B1 expression, leading to increased PGE 2 and decreased PGF 2α concentrations and an increased PGE 2 :PGF 2α ratio. The other tested genes, including PGR, ESR1, OXTR, PTGS2, PTGER2 and PTGFR, were not significantly altered by IFNT, ncpBVDV or their combination. Our study suggests that BVDV infection may impair PR by (1) inhibiting the effect of IFNT on uterine PG production and (2) inducing an endocrine switch of PG production from PGF 2α to PGE 2 to decrease uterine immunity, thereby predisposing the animals to uterine disease.Reproduction (2016) 151 605-614

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Section: Quantification Of Pgs Using Radioimmunoassay (Ria)mentioning

confidence: 99%

BVDV alters uterine prostaglandin production during pregnancy recognition in cows

Cheng¹,

Abudureyimu²,

Oguejiofor³

et al. 2016

Reproduction

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show abstract

“…PGE2 and PGF2 in the spent medium were quantified using charcoal-dextran coated RIA methods as described previously [29]. The samples were diluted (10-200) in the RIA buffer.…”

Section: Pg Radioimmunoassay (Ria)mentioning

confidence: 99%

Effect of oleic acid supplementation on prostaglandin production in maternal endometrial and fetal allantochorion cells isolated from late gestation ewes

et al. 2015

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“…The concentration of PGE 2 was quantified using charcoal-dextran-coated radioimmunoassay as described previously (Cheng et al, 2001). Briefly, standards (0.025-5 ng/ml) or samples were mixed with anti-PGE 2 serum (a kind gift from Dr NL Poyser, University of Edinburgh, Edinburgh, UK) and tritiated tracer ([5,6,8,11,12,14,15(n)-3H]-PGE 2 ; Amersham International, Amersham, UK) in duplicate.…”

Section: Pge 2 Radioimmunoassaymentioning

confidence: 99%

Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion

Marei

Abayasekara

Wathes

et al. 2014

Reproductive BioMedicine Online

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(AA Fouladi-Nashta).Waleed Fawzy A Marei is a veterinarian and graduated from Cairo University, Egypt in 2001. He started his academic career in theriogenology in 2002 and obtained his MSc degree in 2005. He joined the reproduction research group at the Royal Veterinary College, London, UK and investigated the effects of fatty acids on oocyte quality and early embryo development for his PhD, awarded in 2010. He then pursued post-doctoral research in the same group, working on early embryo development and implantation. Currently, he is a lecturer on theriogenology at Cairo University. His interests are clinical applications of assisted reproduction technologies and feed additives to improve reproductive performance in farm animals.Abstract Prostaglandin E 2 (PGE 2 ) is an autocrine/paracrine factor which mediates gonadotrophin (Gn) stimulation of cumulus expansion and oocyte maturation in rodents. Its role in bovine oocyte maturation is less characterized. This study detected PTGS2 (COX2) and PGE synthases (PTGES1, PTGES2 and PTGES3) in bovine cumulus-oocyte complexes (COC). Only PTGS2 and PTGES1 expression changed during maturation. In Gn-free media, no cumulus expansion and $45% nuclear maturation was achieved, while Gn-induced maturation showed full cumulus expansion (score 3) and $87% maturation. PGE 2 supplementation without Gn induced mild cumulus expansion (score 0.5-1) but increased nuclear maturation to levels similar to those obtained with Gn alone. In the presence of Gn, exogenous PGE 2 did not affect expansion or nuclear maturation and subsequent embryo development. Treatment with PTGS2 selective inhibitor (NS398), PTGS2-specific siRNA or PTGER2-receptor antagonist (AH6809) resulted in $20-25% reduction in nuclear maturation. NS398 and AH6809 did not affect cumulus expansion. Most oocytes not reaching metaphase of second meiosis (MII) following NS398, AH6809 and PTGS2-specific siRNA treatments were at MI. After longer maturation, NS398-treated oocytes had normal MII rate and uncompromised embryo development. PGE 2 has a limited role in cumulus expansion in bovine COC but is important for the timing of Gn-induced nuclear maturation. RBMOnline

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Effect of dietary polyunsaturated fatty acids on uterine prostaglandin synthesis in the cow

Abstract: Dietary polyunsaturated fatty acid

Cited by 75 publications

References 67 publications

BVDV alters uterine prostaglandin production during pregnancy recognition in cows

BVDV alters uterine prostaglandin production during pregnancy recognition in cows

Effect of oleic acid supplementation on prostaglandin production in maternal endometrial and fetal allantochorion cells isolated from late gestation ewes

Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion

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