Effect of dietary peNDF levels on digestibility and rumen fermentation, and microbial community in growing goats

Zhou, Jia; Xue, Bine; Hu, Anhai; Yue, Shuangming; Wu, Mei; Hong, Qionghua; Wu, Yuhan; Wang, Zhisheng; Wang, Lizhi; Peng, Quanhui; Xue, Bai

doi:10.3389/fmicb.2022.950587

Cited by 6 publications

(7 citation statements)

References 94 publications

(121 reference statements)

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“…Four diets were formulated with similar ingredients, varying only in the proportions of two types of basal forage of differing chemical composition and particle size. Proper forage particle size is crucial for normal ruminal function and host health as it promotes a stable digesta mat, which increases the retention time of fiber particles, thereby promoting their fermentation by the rumen microbial community and stimulating reticulorumen motility, rumination, and salivation, neutralizing excessive acid production, and preventing a pH drop below levels required for optimal fermentation ( 28 , 29 ). Along with other factors, particle size also plays an important role in feed intake and, thus, in dietary nutrient and energy digestibility.…”

Section: Discussionmentioning

confidence: 99%

“…Changing the acidogenicity value and the physically effective neutral detergent fiber contents of diets are complementary dietary formulation strategies for effectively manipulating the ruminal acid load ( 14 , 33 ), with the carbohydrate type (starch vs. fiber) greatly influencing the ruminal microbial population and the fermentation profile ( 29 , 34 , 35 ). In the present study, these changes were reflected in the alterations in the ruminal fluid fermentation profiles observed when corn silage was replaced with haylage and in the concomitant increase in the neutral detergent fiber-to-starch ratio (1.8 to 10.5).…”

Section: Discussionmentioning

confidence: 99%

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Ruminal inocula with distinct fermentation profiles differentially affect the in vitro fermentation pattern of a commercial algal blend

Mota,

Maia,

Valente

et al. 2024

Front. Vet. Sci.

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The in vitro rumen batch technique is widely used for screening novel feed sources; however, it remains unclear to what extent the in vitro fermentability of non-conventional feed sources is affected by non-adapted ruminal inocula. Thus, in this study, we evaluated the effects of distinct ruminal inocula on the in vitro fermentation parameters of a sustainable non-conventional feed, a commercially available algal blend composed of microalgae (Chlorella vulgaris and Nannochloropsis oceanica) and seaweeds (Ulva sp. and Gracilaria gracilis). First, four late-lactation Holstein cows were fed four forage-based diets varying only in the proportions of basal forage (100% corn silage, 70% corn silage and 30% haylage, 30% corn silage and 70% haylage, and 100% haylage) in a 4 × 4 Latin square design with the last square omitted. After 3 weeks of adaptation, haylage-based diets resulted in ruminal fermentation parameters distinct from those promoted by corn silage-based diets, as reflected in increased pH, ammonia-N contents, and acetate proportions. Individual ruminal fluids derived from each of the four diets were further used as inocula in in vitro incubations. Here, a 1:1 mixture of corn silage and haylage was supplemented with 0, 5, 10, or 15% algal blend and incubated with each inoculum for 24 h in a 4 × 4 factorial design. Total gas and methane production decreased with inocula from cows fed haylage-based diets and with increasing algal blend supplementation levels. The fermentation pH increased and the ammonia-N contents decreased with inocula from cows fed haylage-based diets; however, these parameters were not affected by algal blend inclusion levels. The interaction between the ruminal inoculum source and the algal blend supplementation level affected the total volatile fatty acids (VFA) and the proportions of most individual VFA. Total VFA production decreased with increasing algal supplementation levels, particularly with inocula from cows fed 30% corn silage and 70% haylage; the acetate, propionate, and valerate proportions were only affected by algal blend levels under incubation with 100% corn silage inocula. Overall, our findings highlight the importance of the ruminal inoculum source when assessing the fermentability of non-conventional feed as well as the potential of the algal blend as a natural modulator of ruminal fermentation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ruminal inocula with distinct fermentation profiles differentially affect the in vitro fermentation pattern of a commercial algal blend

Mota,

Maia,

Valente

et al. 2024

Front. Vet. Sci.

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“…The results showed that NAc supplementation did not alter the α-diversity indices (chao1, observed_otus, shannon, simpson, pielou_e), indicating bacterial diversity and richness in the rumen were not affected. The influence of the relative abundance of rumen bacteria is crucial to the apparent digestibility of nutrients and rumen fermentation characteristics, which may significantly affect ruminants’ productive performance and health status ( Ran et al, 2021 ; Zhou et al, 2022 ). Feeding NAc did not affect the α-diversity of the rumen bacterial community, which explains the unaltered rumen NH 3 -N and VFA molar percentages in the NAc treated group.…”

Section: Discussionmentioning

confidence: 99%

“…Dietary variation has an important effect on rumen microbial community composition. Still, the ratio of forage to concentrate and the processing method of the diet are the main factors affecting rumen microbial population structure on the same diet basis ( Henderson et al, 2015 ; Bi et al, 2018 ; Zhou et al, 2022 ). More studies have shown that high grain diets can alter the structure of the rumen microbiota ( Mao et al, 2015 ; Seddik et al, 2019 ; Plaizier et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of supplementation of sodium acetate on rumen fermentation and microbiota in postpartum dairy cows

et al. 2022

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The primary product of rumen fermentation is acetic acid, and its sodium salt is an excellent energy source for post-partum cows to manage negative energy balance (NEB). However, it is unknown how adding sodium acetate (NAc) may affect the rumen bacterial population of post-partum cows. Using the identical nutritional total mixed ration (TMR), this research sought to characterize the impact of NAc supplementation on rumen fermentation and the composition of bacterial communities in post-partum cows. After calving, 24 cows were randomly assigned to two groups of 12 cows each: a control group (CON) and a NAc group (ACE). All cows were fed the same basal TMR with 468 g/d NaCl added to the TMR for the CON group and 656 g/d NAc added to the TMR for the ACE group for 21 days after calving. Ruminal fluid was collected before morning feeding on the last day of the feeding period and analyzed for rumen bacterial community composition by 16S rRNA gene sequencing. Under the identical TMR diet conditions, NAc supplementation did not change rumen pH but increased ammonia nitrogen (NH3-N) levels and microbial crude protein (MCP) concentrations. The administration of NAc to the feed upregulated rumen concentrations of total volatile fatty acids (TVFA), acetic, propionic, isovaleric and isobutyric acids without affecting the molar ratio of VFAs. In the two experimental groups, the Bacteroidota, Firmicutes, Patescibacteria and Proteobacteria were the dominant rumen phylum, and Prevotella was the dominant rumen genus. The administration of NAc had no significant influence on the α-diversity of the rumen bacterial community but upregulated the relative abundance of Prevotella and downregulated the relative abundance of RF39 and Clostridia_UCG_014. In conclusion, the NAc supplementation in the post-peripartum period altered rumen flora structure and thus improved rumen fermentation in dairy cows. Our findings provide a reference for the addition of sodium acetate to alleviate NEB in cows during the late perinatal period.

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“…In addition, the authors observed that cattle fed a diet with cottonseed cake had lower rumination time and bite rate. According to Zhou et al [40], particles ≤ 1.18 mm may have lower digestibility because they have a higher rumen passage rate, mainly affecting fiber digestibility due to the low microbial action.…”

Section: In Vitro Digestibilitymentioning

confidence: 99%

In Vitro Digestibility and Models of Cumulative Gas Production of Forage-Free Diet

Ítavo,

Gurgel,

Ferreira Ítavo

et al. 2023

Animals

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Our objectives were to evaluate the use of cottonseed cake in replacing corn silage in a diet without forage and to identify the model with higher precision and accuracy of adjustment of parameters of ruminal degradation kinetics. A diet containing corn silage and another with cottonseed cake as a fiber source were formulated. Gompertz, Dual-pool Logistic, Brody, and Ørskov models were evaluated for goodness of fit to gas production. There were significant differences in dry matter (DM), organic matter (OM), and neutral detergent fiber (NDF) in the in vitro digestibility for diets and fiber sources. The estimated values of the Gompertz (6.77), Brody (6.72), and Ørskov (6.73) models were similar to the observed mean of gas production in the corn silage diet (6.73 mL/100 mg DM). Similarly, the estimated values of the Brody (5.87) and Ørskov (5.89) models were similar to the observed mean of gas production in the cottonseed cake diet (5.87 mL/100 mg DM). The roughage-free diet containing cottonseed cake as a fiber source stimulated higher gas production. Brody and Ørskov models presented higher precision and accuracy in the fitting of kinetics of degradation independent of the fiber source in the diet.

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Effect of dietary peNDF levels on digestibility and rumen fermentation, and microbial community in growing goats

Cited by 6 publications

References 94 publications

Ruminal inocula with distinct fermentation profiles differentially affect the in vitro fermentation pattern of a commercial algal blend

Ruminal inocula with distinct fermentation profiles differentially affect the in vitro fermentation pattern of a commercial algal blend

Effects of supplementation of sodium acetate on rumen fermentation and microbiota in postpartum dairy cows

In Vitro Digestibility and Models of Cumulative Gas Production of Forage-Free Diet

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