Effect of dietary oils and alpha-tocopheryl acetate supplementation on lipid (TBARS) and cholesterol oxidation in cooked pork.

Abstract

The effect of n-3 fatty acid-enriched diets (in the form of 0.5% linseed oil with either 1.5% sunflower oil or 1.5% olive oil) and alpha-tocopheryl acetate supplementation (200 mg/kg feed) on lipid oxidation (thiobarbituric acid-reactive substances, TBARS) and cholesterol oxide products (COPS) in cooked pork was investigated. Longissimus muscle was studied. Meat from pigs fed 0.5% linseed oil-enriched diets had a higher proportion of n-3 fatty acid than meat from pigs in other dietary groups in neutral (P < 0.0001) and polar lipids (P < 0.0001), and a 20% reduction in the n-6:n-3 ratio was observed. Alpha-tocopheryl acetate supplementation increased (P < 0.05) monounsaturated fatty acids in polar lipids and increased (P = 0.0001) alpha-tocopherol levels in muscle. Alpha-tocopherol concentration in muscle was affected by dietary fat (P < 0.05). Groups receiving diets enriched with sunflower oil had significantly higher alpha-tocopherol levels (P < 0.05) in muscle than those groups receiving olive oil-enriched diets. Numbers of TBARS were significantly (P < 0.05) lower in the group fed supplemental olive oil than in those fed sunflower oil. Dietary linseed oil increased (P < 0.05) lipid oxidation principally at the initial period of storage in cooked pork. Overall, dietary alpha-tocopheryl acetate supplementation significantly increased (P < 0.001) lipid stability and decreased (P < 0.05) total COP production across the dietary groups. Alpha-tocopherol was a more effective antioxidant for decreasing TBARS values in cooked meat when adding sunflower oil to the diets instead of olive oil.

The liver antioxidant status of fattening lambs is improved by naringin dietary supplementation at 0.15% rates but not meat quality

“…After 9 days of storage at 48C, the total COP values were two or three times higher than they were on day 3. Similar data were described previously by Rey et al (2001), who found that the COPs in pork significantly increased during refrigerated storage.…”

“…Indeed, several studies have suggested that cholesterol oxidation is higher in cooked meat from pigs fed n-3-enriched diets, which may lead to more undesirable health effects than potential benefits (Rey et al, 2001). Vitamin E administered to animals has been shown to be effective in reducing COP contents in cooked meat from pigs (Rey et al, 2001;Eder et al, 2005) and chickens (Grau et al, 2001).…”

Effects of dietary inclusion of dehydrated lucerne and whole linseed on rabbit meat quality

“…The effectiveness of whole linseed in increasing the PUFA and a-linolenic acid contents in the meat has been previously reported by several studies on both rabbit (Bernardini et al, 1999;Dal Bosco et al, 2004) and other species (Mattews et al, 2000;Riley et al, 2000;Rey et al, 2001). Moreover, Colin et al (2005) recently proposed the use of a commercial feed ingredient (Tradi-Lin ® ) containing extruded linseed to increase the a-linolenic acid contents of rabbit meat.…”

“…The dietary use of linseed in animal feeds has been proposed by many authors as a vegetable alternative to fish oil or fish meal, to raise the content of n-3 polyunsaturated fatty acids (n-3 PUFA), and mainly a-linolenic acid (C18:3 n-3) in poultry (Ajuyah et al, 1993), pork (Mattews et al, 2000;Rey et al, 2001;Wood et al, 2003) and rabbit meat (Bernardini et al, 1999;Dal Bosco et al, 2004;Colin et al, 2005). Some authors also reported beneficial effects of linseed on performance, milk composition and viability of the progeny in rabbit does.…”

Performance, fatty acids digestibility, carcass and muscle composition of pigs fed diets enriched with vitamin E and differing in their MUFA/PUFA ratio

“…Pentadecanoic acid (C15:1) (Sigma, Alcobendas, Madrid) was used as internal standard. Fatty acid methyl esters were identifi ed by gas chromatography using a 6890 Hewlett Packard (Avondale, PA, USA), gas chromatograph equipped with a fl ame ionization detector and a capillary column HP-Innowax (30 m × 0.32 mm × 0.25 µm cross-linked polyethylene glycol) as described by Rey and Lopez-Bote (2001). Concentration of trans fatty acids in fats used in this experiment (hydrogenated palm fat, lard, olive oil and sunfl ower oil) was quantifi ed by gas chromatography using a 6890 Hewlett Packard (Avondale, PA, USA) gas chromatograph equipped with a fl ame ionization detector and a capillary column (Supelco SP2340 60 × 0,25 mm × 0,2 µm fi lm thickness).…”