Effect of dietary linseed oil and Ganoderma lucidum or olive leaves supplementation on fatty acid composition and oxidative status of rabbits

Abstract

The aim of the present study was to evaluate the influence of natural antioxidant supplementation on the oxidative stress induced by a high proportion of polyunsaturated fatty acids (PUFA) in rabbit diets. Forty-eight SIKA rabbits (24 male, 24 female) were penned individually and after a 5-d adaptation period divided into 4 homogeneous groups (2.58±0.3 kg). The CONT-group received a diet with 60 g/kg palm fat, while the other 3 groups received a diet with 60 g/kg linseed oil which was either unsupplemented (CONT+) or supplemented with 10 g/kg of Ganoderma lucidum (REISHI) or 10 g/kg of olive leaves (OLIVE). Rabbits were euthanised at 102 d of age, 22 d after the start of the experiment. Live weight, weight gain, feed intake and feed conversion rate were recorded. The fatty acid composition of back (M. longissimus dorsi) and hind leg muscle (thigh muscle), adipose tissue (abdominal fat) and liver were determined. To evaluate the oxidative status of rabbits, the malondialdehyde (MDA) concentration in the plasma, liver and both muscles was measured. DNA damage in the leukocytes was measured. Linseed oil addition increased the PUFA ratio and decreased the proportion of saturated fatty acids in all tissues (P<0.001), with no detrimental effect on productive performance of rabbits compared to palm fat. Linseed oil also reduced n-6/n-3 PUFA ratio in all tissues (P<0.001). However, linseed oil addition increased the oxidative stress by increasing MDA concentrations in the liver and muscles (P<0.001), but did not affect the plasma MDA concentrations and the extent of DNA damage in the blood leukocytes compared to palm fat. Addition of olive leaves tended to decrease the concentration of MDA in the liver Addition of olive leaves tended to decrease the concentration of MDA in the liver compared to CONT+ (P=0.059), with no effect of Ganoderma lucidum.

Effect of a dietary supplementation with linseed oil and selenium to growing rabbits on their productive performances, carcass traits and fresh and cooked meat quality

“…Interestingly, values seemed not to be linearly related to the supplementation period (13.4, 0.0, 5.6, 11.1 and 4.2% for B, S1, S2, S3 and S4 rabbits). Similarly to our findings, other researchers did not observe any differences in productive traits when linseed oil was added to the diet of growing rabbits (Maertens et al., ; Trebušak et al., ). Productive traits were also not affected by the dietary supplementation with 0.38 mg Se/kg diet (Dokoupilová et al., ).…”

Performance, fatty acids profile and oxidative stability of meat of rabbits fed different lipid sources

“…Animals fed diet containing beef tallow exhibited higher saturated and lower polyunsaturated fatty acid contents in their meat than those fed soybean oil, demonstrating the quality of the lipid sources and the possibility of altering the fatty acid composition of meat by including these feedstuffs in animal diets to achieve healthier products. Thus, although some researches aimed at increasing meat enrichment in n-3 fatty acids has been focused on the addition of higher-cost lipid sources (Casado et al, 2013;Trebušak et al, 2014), in the present study it is observed that soybean oil, which is commonly used in rabbit diets, has a potential beneficial effect on increase the level of polyunsaturated fatty acids and contribute to the adequate balance of n-6:n-3 ratio of today´s consumer diet.…”

Smart NIR Tomoscopy to Predict Oxidative Stress in Rabbits

“…The risk of oxidative stress was demonstrated by Trebušak et al [25]: after 52 days of unprotected dietary linseed oil administration at a very high level (9% w/w), the malondialdehyde (MDA) was increased by 38% in plasma, 132% in liver, and 210% in muscles. In a second experiment, with 6% dietary linseed oil, after 22 days of feeding Trebušak et al [26] observed an MDA level in plasma that was half that in comparison with their previous trial and which was not changed with linseed oil diet, while, again, the MDA content was increased by 110% in liver and 257% in hindleg meat. In Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 18 January 2019 Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 18 January 2019 doi:10.20944/preprints201901.0188.v1…”