Effect of dietary L‐carnitine supplementation on growth performance and lipid metabolism in<i>Rhynchocypris lagowski</i>Dybowski fed oxidized fish oil

Yu, Ting; Wang, Qiuju; Xiumei, Chen; Chen, Yuke; Ghonimy, Abdallah; Zhang, Dongming; Wang, Guiqin

doi:10.1111/are.14719

“…It was observed that the highest growth performance parameters (WGR, SGR and FE) were observed in mix oil diets with LC (400 mg/kg). The similar growth performance of R. lagowskii was also supported by previous studies (Wang et al, 2019; Yu et al, 2020). Generally, vertebrates have the ability of endogenous synthesis of LC.…”

Section: Discussionsupporting

confidence: 87%

Effects of L‐carnitine supplementation on growth performance, histomorphology, antioxidant and immune function of Rhynchocypris lagowskii fed dietary fish oil replaced with corn oil

Wang

¹

,

Yao

²

,

Wang

³

et al. 2022

Aquaculture Research

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The effects of L‐carnitine (LC) and dietary lipid on the growth and health of farmed fish are still conflicting. This study aimed to explore the effects of LC supplementation and dietary fish oil replacement by corn oil on growth performance, antioxidant, immune function and histomorphology of Rhynchocypris Lagowskii. Six diets were evaluated as follows: dietary fish oil as 50% and 100% replacement by corn oil, and differing in LC supplementation (0 and 400 mg/kg), were tested in R. Lagowskii for 8 weeks. The highest weight gain rate, specific growth rate and feed efficiency were observed in mix oil diets with LC. The diet with LC significantly increased the total antioxidant capacity level, total superoxide dismutase, alkaline phosphatase, lysozyme activities and immunoglobulin M, complement levels in liver and intestine compared with corn oil diet (p < 0.05). Moreover, the diet with LC significantly up‐regulated the nuclear factor E2‐related factor 2 (p < 0.05), and significantly down‐regulated the interleukin‐1‐beta and tumour necrosis factor‐alpha expression in liver and intestine compared with corn oil diet (p < 0.05). In conclusion, LC supplementation improved the weakening of growth performance, antioxidant capacity, immune function and tissue health caused by R. lagowskii intake of corn oil diets.

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“…However, EPA and DHA are easily oxidized during the storage and processing of fish oil and feed, producing harmful substances such as lipid hydroperoxides, ketones, aldehydes, and acids [ 4 , 5 ]. Studies have also reported that feeding an oxidized-fish-oil diet has been found to decrease growth performance and cause oxidative stress of tilapia ( Oreochromis niloticus ) [ 6 ], Wuchang bream ( Megalobrama amblycephala ) [ 7 ], and orange spotted grouper ( Epinephelus coioides ) [ 5 ], disrupt lipid metabolism of Rhynchocypris lagowski Dybowski [ 8 ], and induce intestinal injury of Megalobrama amblycephala [ 9 ] and rice field eel ( Monopterus albus ) [ 10 ]. Therefore, exploring effective dietary strategies is imperative to alleviate the negative effects of oxidized-fish-oil diets on aquatic animals.…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Taurine on Oxidized Fish-Oil-Induced Liver Oxidative Stress and Intestinal Barrier-Function Impairment in Juvenile Ictalurus punctatus

Shi

¹

,

Hu

²

,

Wang

³

et al. 2021

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Dietary lipids provide energy for growth and development and provide fatty acids necessary for normal structure and biological function. However, oxidized lipids cause oxidative stress and intestinal damage. An 8-week feeding trial with fresh fish oil (FFO, control group), oxidized fish oil (OFO), and taurine-supplemented diets (OFOT, OFO + 0.2% of taurine) was conducted to evaluate the protective effect of taurine on oxidized fish-oil-induced liver oxidative stress and intestine impairment in juvenile Ictaluruspunctatus. The results showed that (1) Growth performance was significantly lower in fish fed OFO than in those fed other diets, whereas the opposite occurred in the hepatosomatic index. (2) OFO-feeding significantly increased lipid deposition compared with the FFO group. The addition of taurine ameliorated the OFO-induced increase in lipid vacuolization in the liver, significantly upregulated lpl mRNA expression, and downregulated fas and srebp1 mRNA expression. (3) OFO-feeding significantly reduced oxidative damage of liver. Compared with the OFO group, the OFOT group remarkably upregulated antioxidant enzyme mRNA expression through the Nrf2-Keap1 signaling pathway based on the transcriptional expression. (4) OFO diets induced intestinal physical and immune barrier damage. Compared with the OFO group, OFOT diets remarkably downregulated il-1β, il-6, tnf-α, and il-8 mRNA expression and upregulated tgf-β mRNA expression through the NF-κB signaling pathway. Besides, the addition of taurine to OFO diets significantly upregulated zo-2 and zo-1 mRNA expression, and downregulated claudin-15 and claudin-12 mRNA expression. In conclusion, oxidized-fish-oil diets can cause negative physiological health effects in Ictaluruspunctatus, while adding taurine can increase growth and antioxidant ability, reduce lipid deposition, and improve intestinal health.

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“…Primers are shown in Table 2. The analysis was conducted according to the 2 -ΔΔCT methodology of a previous study [19].…”

Section: Analysis Of Immune and Antioxidantmentioning

confidence: 99%

“…The Amur minnow (Rhynchocypris lagowskii Dybowski) is an omnivorous cold-water fish with fresh and tender meat. It is one of the main economic fish promoted in Northeast China in recent years [19]. Countries have completely banned antibiotics worldwide [1].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Bacillus velezensis LSG2-5 on Growth, Immunity, Antioxidant Capacity, and Disease Resistance of Amur minnow (Rhynchocypris lagowskii Dybowski)

Zhang

¹

,

Yu

²

,

Lin³

et al. 2022

Aquaculture Nutrition

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The study was aimed at investigating the effects of Bacillus velezensis LSG2-5 isolated from host’s intestinal tract on growth performance, immune response, antioxidant capacity, and disease resistance of Amur minnow (Rhynchocypris lagowskii Dybowski). 535 fish were divided into 5 groups with 3 replicates per group. Diets containing different doses (0, 106, 107, 108, and 109 CFU/g, respectively) of B. velezensis LSG2-5 were used to feed fish ( 9.95 ± 0.05 g) for 56 days. Results showed that compared with control, weight gain rate and specific growth rate of fish were significantly increased by dietary 107-109 CFU/g B. velezensis ( p < .05 ). While for the feed conversion ratio, all treatment groups were significantly decreased ( p < .05 ). In serum, the contents of lysozyme and immunoglobulin M were significantly increased by dietary 106-109 CFU/g B. velezensis ( p < .05 ); the activities of component 3, component 4, and alkaline phosphatase were significantly increased by dietary 108 CFU/g B. velezensis ( p < .05 ). The activities of catalase, glutathione peroxidase, total antioxidant capacity, and superoxide dismutase in hepatopancreas were significantly increased by dietary 108-109 CFU/g B. velezensis ( p < .05 ). The activities of catalase, glutathione, glutathione peroxidase, total antioxidant capacity, and superoxide dismutase in the intestinal tract of 108 CFU/g group were significantly increased ( p < .05 ). However, malondialdehyde levels in the hepatopancreas and intestines were significantly decreased by dietary 107-108 CFU/g B. velezensis ( p < .05 ). Dietary 107-109 CFU/g B. velezensis significantly upregulated catalase, glutathione peroxidase, and copper/zinc superoxide dismutase mRNA levels ( p < .05 ). The levels of transforming growth factor β and interleukin-10 mRNA in the hepatopancreas of fish fed with B. velezensis of 108 CFU/g were significantly increased ( p < .05 ), and these in the intestinal tract of 106-109 CFU/g groups were upregulated ( p < .05 ). Feeding dietary 107-108 CFU/g B. velezensis significantly downregulated interleukin-8, interleukin-1β, and tumour necrosis factor α mRNA levels in hepatopancreas and intestines ( p < .05 ). After 56 days of feeding, 225 fish ( 23.20 ± 0.59 g) were challenged with Aeromonas hydrophila (108 CFU/g), and survival rates were recorded for the next 14 days. Meanwhile, the serum immune parameters of fish were determined. Results showed that compared with control, the survival rates of the treatment groups were significantly increased ( p < .05 ). Lysozyme, component 3, component 4, immunoglobulin M, acid phosphatase, and alkaline phosphatase levels in serum by dietary106-109 CFU/g B. velezensis showed significant promoting effects ( p < .05 ). In conclusion, dietary 107-109 CFU/g B. velezensis boosts the growth, immunity, antioxidant capacity, and against A. hydrophila infection of Amur minnow. Regression analysis suggested that the optimal dose of B. velezensis LSG2-5 in the Amur minnow diet was 5.00 × 10 8 CFU/g.

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Effect of dietary L‐carnitine supplementation on growth performance and lipid metabolism inRhynchocypris lagowskiDybowski fed oxidized fish oil

Cited by 13 publications

References 34 publications

Effects of L‐carnitine supplementation on growth performance, histomorphology, antioxidant and immune function of Rhynchocypris lagowskii fed dietary fish oil replaced with corn oil

Effects of L‐carnitine supplementation on growth performance, histomorphology, antioxidant and immune function of Rhynchocypris lagowskii fed dietary fish oil replaced with corn oil

The Protective Effect of Taurine on Oxidized Fish-Oil-Induced Liver Oxidative Stress and Intestinal Barrier-Function Impairment in Juvenile Ictalurus punctatus

Effects of Dietary Bacillus velezensis LSG2-5 on Growth, Immunity, Antioxidant Capacity, and Disease Resistance of Amur minnow (Rhynchocypris lagowskii Dybowski)

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