Effect of dietary isoleucine level on growth, protein retention efficiency, haematological parameter, lysozyme activity and serum antioxidant status of fingerlingChanna punctatus(Bloch)

Abstract

Isoleucine requirement of fingerling Channa punctatus (6.74 ± 0.09 g) was estimated by feeding seven trial diets (450 g/kg CP, 14.73 kJ/g DE) containing 3.8, 7.5, 11.3, 15.1, 19.3, 23.2 and 27.4 g/kg of isoleucine for 12 weeks. Growth and haematological parameters increased with the increasing concentrations of dietary isoleucine up to 16 g/kg. Carcass protein and fat increased significantly with the increasing concentrations of dietary isoleucine up to 16 g/kg and then stabilized. Moisture content showed reverse trend to that of carcass fat. Hepatosomatic index was found to be highest at 4 g/kg of dietary isoleucine. Viscerosomatic index and condition factor increased significantly with increasing levels of isoleucine up to 16 g/kg dry diet. Serum protein, lysozyme and superoxide dismutase activities were also found to increase significantly up to 16 g/kg dry diet. Significant reduction in alanine aminotransferase and aspartate aminotransferase activities was observed by increasing concentrations of dietary isoleucine up to 16 g/kg. Based on quadratic regression analysis of absolute weight gain, feed conversion ratio, protein retention and isoleucine retention efficiencies against varying concentrations of isoleucine, the optimum isoleucine requirement ranging between 17.95 and 18.39 g/kg dry diet, corresponding to 39.88–40.86 g/kg dietary protein, is recommended for maximizing growth of C. punctatus.

Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli ♀ × Leiocassis longirostris♂)

“…In present result, dietary Ile signi cantly up-regulated muscle protein content. Similar results were observed in ngerling spotted snakehead [14], grass carp [9], and Nile tilapia [59]. Previous studies have observed that IGF1-PI3K-AKT acts a positive regulator in protein synthesis in mammals [77].…”

Dietary isoleucine improved flesh quality, muscle antioxidant capacity, and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish (Pelteobagrus vachelli♀ × Leiocassis longirostris♂)

“…The Ile, one of BCAA, have been reported the importance of effecting fish growth performance [ 9 , 14 , 59 ]. Fish growth is mainly determined by muscle growth, which constitutes the major edible sources of the fillet [ 6 , 8 ].…”

“…The nutritional content was mainly reflected by muscle protein and lipid contents. Emerging shreds of evidence have shown that dietary Ile could increase muscle protein and lipid contents of grass carp ( Ctenopharyngodon idella ) [ 9 ] and fingerling spotted snakehead ( Channa punctatus ) [ 14 ]. These results provided possible evidence that dietary Ile could affect muscle nutritional content and merit further study.…”

Interactive effect between dietary valine and isoleucine on growth performance, blood parameters and resistance against low salinity stress of Japanese flounderParalichthys olivaceus

“…Six experimental diet were produced as follows: minimum level of Ile with minimum level of Val (LI‐LV), minimum of Ile with medium of Val, minimum of Ile with maximum of Val, maximum of Ile with minimum of Val (HI‐LV), maximum of Ile with medium of Val, maximum of Ile with maximum of Val (HI‐HV). Crystalline amino acid (CAA) mixture, 10% casein, 5% gelatin, and 20% fishmeal were supplemented to these six experimental diets to provide an amino acid profile in associated with 50% whole body protein of Japanese flounder (shown in Table 2; Alam et al., 2007; Han et al., 2013a; Sharf & Khan, 2020). And dispensable amino acid were supplemented to these six experimental diets to maintain it as isonitrogenous diet (Han, Koshio, et al., 2014).…”

“…Val is another BCAAs which plays an important role in certain biochemical reactions and the growth performnance of monogastric animals (Bae et al., 2012; Sharf & Khan, 2020). Previous studies already established the optimum requirements of Val in aquatic animals, for Japanese flounder, the optimal requirement of Ile is 2.5% of protein (Sharf & Khan, 2020). Moreover, Val was reported to increase the growth of juvenile Jian carp ( Cyprinus carpiovar .…”