Effect of Dietary Inorganic Copper on Growth Performance and Some Hematological Indices of Siberian Sturgeon Acipenser baerii Juveniles

Abstract

This research work was conducted to evaluate the effect of dietary copper (Cu) levels on growth performance, feed utilization, and some hematological parameters of juvenile Siberian Sturgeon Acipenser baerii. Five semipurified diets containing 0, 3, 6, 12, and 24 mg Cu/kg in the form of copper sulfate pentahydrate (CuSO 4 •5H 2 O) were formulated to provide the actual dietary copper values of 1.8, 5.7, 10.1, 15.9, and 28.3 mg Cu/kg diet, respectively. The obtained results exhibited a significant improvement in the final weight, protein efficiency ratio, daily growth coefficient, and hepatosomatic index with increasing dietary copper levels up to 10.1 mg/kg diet, while the feed conversion ratio significantly decreased. Also, erythrocyte count, hemoglobin, and hematocrit values increased with increasing levels of dietary copper, but no steady trend was observed in mean corpuscular hemoglobin changes. Furthermore, the neutrophils were significantly increased with enhancing the copper level in the fish diet, while the lymphocytes declined. Findings of the current research illustrate that an appropriate dietary copper level is required for improvement of growth performance, oxygen transfer capability, and white blood cell proliferation. Based on the broken-line regression analysis of leukocyte count and monocyte percentage, the optimum dietary copper supplementation was estimated to be 10.63 and 10.36 mg Cu/kg diet, respectively.Copper (Cu) is an essential micromineral necessary for all animals, including fish, which functions in variety of biological processes, such as hematopoiesis, bone formation, cellular respiration, redox reactions, neurotransmitter synthesis, and maintenance of myelin within the nervous system (

Effects of dietary nanoliposome‐coated astaxanthin on haematological parameters, immune responses and the antioxidant status of rainbow trout (Oncorhynchus mykiss)

“…Haemoglobin concentration (Hb, g dL −1 ) was determined based on the cyanomethemoglobin method by measuring a wavelength of 540 nm (Ahmadpanah et al., 2019 ). The derived blood indices, including mean cell volume (MCV, fL) as the average size of erythrocytes, mean cell haemoglobin (MCH, pg) as the weight of haemoglobin in the average RBC and (MCHC, g dL −1 ) as the relationship between the size of erythrocytes and their haemoglobin contents, were determined using the following formulae (Moazenzadeh et al., 2020 ): …”

“…Haemoglobin concentration (Hb, g dL −1 ) was determined based on the cyanomethemoglobin method by measuring a wavelength of 540 nm (Ahmadpanah et al., 2019). The derived blood indices, including mean cell volume (MCV, fL) as the average size of erythrocytes, mean cell haemoglobin (MCH, pg) as the weight of haemoglobin in the average RBC and (MCHC, g dL −1 ) as the relationship between the size of erythrocytes and their haemoglobin contents, were determined using the following formulae (Moazenzadeh et al., 2020): $$$$\begin{equation*} \def\eqcellsep{&}\begin{array}{l} \def\eqcellsep{&}\begin{array}{@{}*{1}{l}@{}} {{\mathrm MCV} = 10 \times {\mathrm Hct} /{\mathrm RBC} }\\ {{\mathrm MCH} = 10 \times {\mathrm Hb} /{\mathrm RBC} } \end{array} \\ {\mathrm MCHC} = 100 \times {\mathrm Hb} /{\mathrm Hct} \end{array} \end{equation*}$$$$…”