Effect of dietary inclusion of dried apple pomace on faecal butyrate concentration and modulation of gut microbiota in dogs

Brito, Cleusa Bernardete Marcon de; Souza, Camilla Mariane Menezes; Bastos, Taís Silvino; Mesa, Dany; Oliveira, Simone Gisele de; Félix, Ananda Portella

doi:10.1080/1745039x.2020.1867463

Cited by 10 publications

(11 citation statements)

References 33 publications

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“…Certain feed additives can improve and stabilize the composition of the intestinal microbiota, reducing the presence of pathogens as well as toxins [ 27 ]. In addition, prebiotics may lead to enhanced immune function and can be used safely as a secondary treatment of diseases, such as certain bacterial intestinal infections, constipation, kidney or liver diseases, especially hepatic encephalopathy caused by PSS, explaining the increasing interest in the application of fiber and prebiotics such as lactulose and psyllium in animal feed [ 11 , 28 , 29 , 30 , 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Altered Intestinal Production of Volatile Fatty Acids in Dogs Triggered by Lactulose and Psyllium Treatment

Mackei

Talabér

Müller

et al. 2022

Veterinary Sciences

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The intestinal microbiome of dogs can be influenced by a number of factors such as non-starch polysaccharides as well as some non-digestible oligo- and disaccharides. These molecules are only decomposed by intestinal anaerobic microbial fermentation, resulting in the formation of volatile fatty acids (VFAs), which play a central role in maintaining the balance of the intestinal flora and affecting the health status of the host organism. In the present study, the effects of lactulose and psyllium husk (Plantago ovata) were investigated regarding their influence on concentrations of various VFAs produced by the canine intestinal microbiome. Thirty dogs were kept on a standard diet for 15 days, during which time half of the animals received oral lactulose once a day, while the other group was given a psyllium-supplemented diet (in 0.67 and in 0.2 g/kg body weight concentrations, respectively). On days 0, 5, 10 and 15 of the experiment, feces were sampled from the rectum, and the concentration of each VFA was determined by GC-MS (gas chromatography–mass spectrometry). Lactulose administration caused a significant increase in the total VFA concentration of the feces on days 10 and 15 of the experiment (p = 0.035 and p < 0.001, respectively); however, in the case of psyllium supplementation, the concentration of VFAs showed a significant elevation only on day 15 (p = 0.003). Concentrations of acetate and propionate increased significantly on days 5, 10 and 15 after lactulose treatment (p = 0.044, p = 0.048 and p < 0.001, respectively). Following psyllium administration, intestinal acetate, propionate and n-butyrate production were stimulated on day 15, as indicated by the fecal VFA levels (p = 0.002, p = 0.035 and p = 0.02, respectively). It can be concluded that both lactulose and psyllium are suitable for enhancing the synthesis of VFAs in the intestines of dogs. Increased acetate and propionate concentrations were observed following the administration of both supplements; however, elevated n-butyrate production was found only after psyllium treatment, suggesting that the applied prebiotics may exert slightly different effects in the hindgut of dogs. These findings can be also of great importance regarding the treatment and management of patients suffering from intestinal disorders as well as hepatic encephalopathy due to portosystemic shunt.

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Section: Discussionmentioning

confidence: 99%

Altered Intestinal Production of Volatile Fatty Acids in Dogs Triggered by Lactulose and Psyllium Treatment

Mackei

Talabér

Müller

et al. 2022

Veterinary Sciences

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“…Interestingly, Negativibacillus relative abundance decreased from baseline following AJ+P intervention and was also inversely correlated with baseline TDF intake in our cross-sectional analysis. In canines, an increase in relative abundance of Faecalibacterium was observed following consumption of a diet containing 9% dried apple pomace (43) .…”

Section: Discussionmentioning

confidence: 99%

Bowel habits, faecal microbiota and faecal bile acid composition of healthy adults consuming fruit pomace fibres: two-arm, randomised, double-blinded, placebo-controlled trials

Alexander

Brauchla

Sanoshy³

et al. 2022

Br J Nutr

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Dietary fiber modulates gastrointestinal (GI) health and function, providing laxation, shifting microbiota, and altering bile acid (BA) metabolism. Fruit juice production removes the polyphenol- and fiber-rich pomace fraction. The effects of orange and apple pomaces on GI outcomes were investigated in healthy, free-living adults. Healthy adults were enrolled in two double-blinded, crossover trials, being randomized by baseline bowel movement (BM) frequency. In the first trial, subjects (n=91) received orange juice (OJ, 0g fiber/d) or OJ + orange pomace (OJ+P, 10g fiber/d) for 4 wk, separated by a 3-wk washout. Similarly, in the second trial, subjects (n=90) received apple juice (AJ, 0g fiber/d) or AJ + apple pomace (AJ+P, 10g fiber/d). Bowel habit diaries, GI tolerance surveys, and 3-d diet records were collected throughout. Fresh fecal samples were collected from a participant subset for microbiota and BA analyses in each study. Neither pomace interventions influenced BM frequency. At wk 4, OJ+P tended to increase (p=0.066) GI symptom occurrence compared to OJ, while AJ+P tended (p=0.089) to increase flatulence compared to AJ. Faecalibacterium (p=0.038) and Negativibacillus (p=0.043) were differentially abundant between pre- and post-interventions in the apple trial, but were no longer significant after FDR correction. Baseline fiber intake was independently associated with several microbial genera in both trials. Orange or apple pomace supplementation was insufficient to elicit changes in bowel habits, microbiota diversity, or BA of free-living adults with healthy baseline BM. Future studies should consider baseline BM frequency and habitual fiber intake.

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“…The SF fraction is an important substrate for gut microbiota fermentation, contributing to the maintenance of eubiosis and resulting in the production of functional metabolites, such as short‐chain fatty acids (SCFA). The SCFA are an important energy source for colonocytes (especially butyrate), regulate epithelial barrier integrity and water and electrolytes absorption, support the immune system and modulate inflammatory responses (de Brito et al, 2021; Panasevich et al, 2013; Volpe et al, 2021). On the other hand, the IF fraction stimulates peristalsis and can alter faecal consistency (Kröger et al, 2017; Sabchuk et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

“…Although studies already have demonstrated the physiological effects of fibre sources, such as beet pulp, cellulose, soy hulls and fruit pomaces on the gastrointestinal functionality of dogs (de Brito et al, 2021; Donadelli & Aldrich, 2019; Donadelli et al, 2021; Pacheco et al, 2021; Panasevich et al, 2015; Sabchuk et al, 2017; Souza et al, 2021; Volpe et al, 2021), only one study (Souza et al, 2021) evaluated the effects of CA in this species. The study of Souza et al (2021) demonstrated that CA is fermentable in the gut, altering the production of fermentative metabolites and changing the gut microbiota of dogs.…”

Section: Introductionmentioning

confidence: 99%

Fine cassava fibre utilization as a dietary fibre source for dogs: Effects on kibble characteristics, diet digestibility and palatability, faecal metabolites and microbiota

Souza

Bastos

Kaelle

et al. 2023

Animal Physiology Nutrition

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The objective was to evaluate through three experiments the effects of a fine cassava fibre (CA: 106 μm) on kibble characteristics, coefficients of total tract apparent digestibility (CTTAD) of macronutrients, diet palatability and faecal metabolites and microbiota of dogs. Dietary treatments consisted of a control diet (CO), without an added fibre source and with 4.3% total dietary fibre (TDF), and a diet with 9.6% CA (106 μm), with 8.4% TDF. Experiment I evaluated the physical characteristics of the kibbles. The palatability test was evaluated in experiment II, which compared the diets CO versus CA. In experiment III, 12 adult dogs were randomly assigned to one of the two dietary treatments for 15 days, totalling six replicates/treatment, to assess the CTTAD of macronutrients; faecal characteristics, faecal metabolites and microbiota. The expansion index, kibble size and friability of diets with CA were higher than the CO (p < 0.05). Additionally, the CA diet presented higher palatability than the CO (p < 0.05) but did not affect CTTAD except for those of fibre (p > 0.05). Moreover, a greater faecal concentration of acetate, butyrate and total short-chain fatty acids (SCFA) and a lower faecal concentration of phenol, indole and isobutyrate were observed in dogs fed the CA diet (p < 0.05). Dogs fed with the CA diet presented a greater bacterial diversity and richness and a greater abundance of genera considered to be beneficial for gut health, such as Blautia, Faecalibacterium and Fusobacterium when compared to the CO group (p < 0.05). The inclusion of 9.6% of a fine CA improves the expansion of kibbles and diet palatability without affecting most of the CTTAD of nutrients. Besides, it improves the production of some SCFA and modulates the faecal microbiota of dogs.

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Effect of dietary inclusion of dried apple pomace on faecal butyrate concentration and modulation of gut microbiota in dogs

Cited by 10 publications

References 33 publications

Altered Intestinal Production of Volatile Fatty Acids in Dogs Triggered by Lactulose and Psyllium Treatment

Altered Intestinal Production of Volatile Fatty Acids in Dogs Triggered by Lactulose and Psyllium Treatment

Bowel habits, faecal microbiota and faecal bile acid composition of healthy adults consuming fruit pomace fibres: two-arm, randomised, double-blinded, placebo-controlled trials

Fine cassava fibre utilization as a dietary fibre source for dogs: Effects on kibble characteristics, diet digestibility and palatability, faecal metabolites and microbiota

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