Effect of dietary fish oil intake on ubiquitin ligase expression during muscle atrophy induced by sciatic nerve denervation in mice

Abstract

Dietary fish oil intake improves muscle atrophy in several atrophy models however the effect on denervation-induced muscle atrophy is not clear. Thus, the aim of this study was to investigate the effects of dietary fish oil intake on muscle atrophy and the expression of muscle atrophy markers induced by sciatic nerve denervation in mice. We performed histological and quantitative mRNA expression analysis of muscle atrophy markers in mice fed with fish oil with sciatic nerve denervation.Histological analysis indicated that dietary fish oil intake slightly prevented the decrease of muscle fiber diameter induced by denervation treatment. In addition, dietary fish oil intake suppressed the MuRF1 (tripartite motif-containing 63) expression up-regulated by denervation treatment, and this was due to decreased tumor necrosis factor-alpha (TNF-α) production in skeletal muscle. We concluded that dietary fish oil intake suppressed MuRF1 expression by decreasing TNF-α production during muscle atrophy induced by sciatic nerve denervation in mice. K E Y W O R D Sfish oil, sciatic nerve denervation, skeletal muscle atrophy, TNF-α, ubiquitin ligase

Characterization of Undiscovered miRNA Involved in Tumor Necrosis Factor Alpha-Induced Atrophy in Mouse Skeletal Muscle Cell Line

“…According to the current hypothesis, inflammation plays a significant role in the onset and progression of skeletal muscle wasting [ 20 ]. Recently accumulated evidence indicates that a number of cytokines, most notably the pro-inflammatory cytokine, TNF-α, may promote muscular atrophy [ 21 , 22 ]. Inflammatory response and muscle condition undergo complex regulation by non-coding RNA machinery.…”

DMHF (2,5‐dimethyl‐4‐hydroxy‐3(2H)‐furanone), a volatile food component generated by the Maillard reaction, promotes appetite and changes gene expression in the rat brain through inhalation

“…Real‐time quantitative RT‐PCR was performed as described previously (Komiya et al., 2019). Total RNA was extracted from whole rat brains using TRIzol reagent, and reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen, CA, USA) and Oligo d(T)12‐18 Primer (Applied Biosystems, MA, USA) according to the manufacturers’ protocols.…”

Nutraceuticals in the Prevention and Treatment of the Muscle Atrophy

“…Dietary supplementation of menhaden fish oil, mainly containing omega-3 unsaturated fatty acids and docosahexaenoic acid, could suppress lipopolysaccharide-induced muscle atrophy in piglets through the modulation of Akt/FoxO, Toll-like receptors and nucleotide-binding oligomerization domain protein signaling pathways [76]. Recently, in another study, dietary menhaden fish oil intake suppresses MuRF1 expression by decreasing TNF-α production in denervation-induced muscle atrophy in C57BL/6J mice [77]. Moreover, a randomized controlled trial suggests that fish oil-derived omega-3 unsaturated fatty acids increase muscle mass and function in healthy older adults [78].…”