Effect of dietary eicosapentaenoic and docosahexaenoic acid on expression of rat liver genes controlling cholesterol homeostasis and on plasma cholesterol level

Komprda, T.; Zorníková, Gabriela; Knoll, A.; Vykoukalová, Z.; Rozikova, Veronika; Škultéty, O.; Krobot, R

doi:10.17221/7650-cjas

Cited by 12 publications

(11 citation statements)

References 31 publications

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Section: Discussionsupporting

confidence: 93%

“…The effect of fish oil on total plasma cholesterol found out in the present experiment (significant decrease in comparison with the palm oil control; Figure 1) agrees with the results of our previous experiment in rats (Komprda et al 2014). However, a response of pigs (present experiment) and rats (Komprda et al 2014) on dietary fish oil in comparison with palm oil was totally different as far as plasma TAG are concerned: no difference ( Figure 1) and a substantial (twofold) decrease, respectively. An explanation is likely based on the differences between rats and pigs in a response to PUFA n-3 as natural ligands of various isoforms of peroxisome proliferator-activated receptors (PPAR); rats and pigs are labelled in this context as proliferating and non-proliferating species, respectively (Komprda 2012).…”

Section: Discussionsupporting

confidence: 93%

“…Chen et al (2012) reported no significant effect of DHA oil in comparison with beef tallow on the body weight gain in weaned pigs, which agrees with the findings of the present experiment. Similarly, dietary intervention had no significant effect on either daily weight gain or the final live weight of rats fed a diet with fish oil and palm oil, respectively, in our previous experiment (Komprda et al 2014). Fatty acid deposition in the tissues.…”

Section: Discussionmentioning

confidence: 62%

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Tissue fatty acid deposition, plasma lipid and cytokine profile in pigs fed a diet with fish oil or palm oil

Komprda¹,

Rozikova²,

Vícenová³

et al. 2017

CZECH J ANIM SCI

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Komprda T., Rozíková V., Vícenová M., Procházková N., Ondráčková P., Pešková P., Faldyna M. (2017): Tissue fatty acid deposition, plasma lipid and cytokine profile in pigs fed a diet with fish oil or palm oil. Czech J. Anim. Sci., 62, 482-490.The present study tested a hypothesis concerning a favourable effect of dietary fish oil on the tissue polyunsaturated fatty acid (PUFA) deposition, and on plasma lipid and cytokine profile. Thirty-two pigs divided into two groups of 16 animals each were fed for 70 days a diet with 2.5% of fish oil (F) and palm oil (P), respectively. The content of PUFA n-3 in the liver, muscle (m. quadriceps femoris), and visceral adipose tissue (VAT) of the F-and P-pigs was 530 and 129, 84 and 19, 1365 and 191 mg/100 g of the fresh tissue, respectively (differences between dietary groups were significant at P < 0.01 in all tissues). Dietary fish oil in comparison with palm oil decreased (P < 0.05) total plasma cholesterol, but also desirable high-density lipoprotein cholesterol, and had no effect (P > 0.05) both on low-density lipoprotein cholesterol and triacylglycerols. Moreover, dietary fish oil increased (P < 0.05) expression of the genes coding for not only anti-inflammatory cytokines IL-10 (P < 0.01) and TGF-β1 (P < 0.05), but also for pro-inflammatory cytokines IL-6 (P < 0.01), IL-12, and TNF-α (P < 0.05) in the VAT, and increased (P < 0.05) the expression of the IL6 gene in the liver. On the other hand, no significant differences (P > 0.05) between the F-and P-pigs in plasma levels of any tested cytokine were found out. It was concluded that an effect of dietary fish oil on tissue fatty acid deposition is undeniable, but its effects on plasma markers related to the risk of chronic degenerative diseases require further research.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 62%

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Tissue fatty acid deposition, plasma lipid and cytokine profile in pigs fed a diet with fish oil or palm oil

Komprda¹,

Rozikova²,

Vícenová³

et al. 2017

CZECH J ANIM SCI

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“…2), fish oil in the rat diet decreased plasma TC also in most recent studies (e.g. Lu et al 2011;Campioli et al 2012;Komprda et al 2014). The TC value established in the present experiment after feeding the F1 diet (0.98 mmol·l -1 ) is the same as found in the experiment of Takahashi (2011).…”

Section: Discussionsupporting

confidence: 87%

“…Total plasma cholesterol, LDL-cholesterol, HDL-cholesterol and triacylglycerols were determined according to Komprda et al (2014) by the enzymatic-colorimetric method using an automated chemical analyser BS-200 (Mindray, China) and commercial kits (Greiner Diagnostic GmbH, Germany). C -control diet (basic feed mixture for mice and rats with 7% [w/w] of maize starch); T -basic feed mixture with 20% of beef tallow + 40% of evaporated sweetened milk + 2 % of extra vitamins/minerals premix; P -basic feed mixture with 5% of palm oil; SF -basic feed mixture with 5% of safflower oil; S -basic feed mixture with 5% of salmon oil; F -basic feed mixture with 5% of fish oil (commercial oleum jecoris aselli); A -basic feed mixture with 5% of oil extracted from the Schizochytrium microalga…”

Section: Plasma Lipids Determinationmentioning

confidence: 99%

The effect of dietary fatty acid composition on the hepatic fatty acid content and plasma lipid profile in rats

et al. 2015

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The objective of the present study was to evaluate in a model organism the effect of different dietary lipids on plasma concentration of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triacylglycerols (TAG). One hundred adult male rats (Wistar Albino) were divided into 10 groups with 10 animals each and fed for 7 weeks either basic feed mixture (control diet, C) or basic feed mixture with 5% of palm oil (P), safflower oil (SF), salmon oil (S), fish oil (F), Schizochytrium microalga oil (A), and 20% of beef tallow (T; four groups), respectively. The T-groups were fed for another 7 weeks T-, SF-, F-and A-diet, respectively. At the end of both the first and the second 7-week fattening period, plasma lipid concentration and hepatic fatty acid content was determined. Both A and F diets fed for 7 weeks decreased (P < 0.05) plasma TC (0.98 mmol•l ). The highest (P < 0.05) plasma TC was established in rats fed for 7 weeks the SF-diet following the previous 7-week T-treatment (2.15 mmol•l -1 ). A-diet had the most positive (decreasing) effect on TAG concentrations (0.68-0.86 mmol•l -1 compared to 1.22 and 2.88 mmol•l -1 found in the C and T diets, respectively; P < 0.05). Both plasma TC and TAG were in a negative relationship (P < 0.01) with the hepatic eicosapentaenoic acid and docosahexaenoic acid (DHA) contents, respectively. It was concluded that dietary Schizochytrium microalga oil (with high DHA content) may have the potential for decreasing the risk of cardiovascular diseases.

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Effects of acute heat stress on haemato-biochemical parameters, oxidative resistance ability, and immune responses of hybrid yellow catfish (pelteobagrus fulvidraco × P. vachelli) juveniles

et al. 2023

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This study investigated the effect of heat stress on the physiological parameters, oxidation resistance ability and immune responses in juvenile hybrid yellow cat sh. Heat stress group exposed to 35°Cand control to 28°C. Blood and liver were sampled at different hours' post-exposure. Results showed that red blood cell (RBC), white blood cell (WBC) counts, Hemoglobin (HGB) levels and hematocrit (HCT) values increased signi cantly (P < 0.05) post-exposure to heat stress. This indicates the increase of cell metabolism. Serum alanine aminotransferase (ALT) and aspartate transaminase (AST) activities, total cholesterol (TC), total protein (TP), triglyceride (TG) and glucose increased significantly (P < 0.05) indicating the need to cope with stress and cell damage. Liver TC, TG, COR hormone, C3 complement increased signi cantly from 24h to 96h. Heat stress mostly affects the hepatic antioxidant and immune resistance functions, resulting in increments of cortisol levels, lysozyme, superoxide dismutase (SOD), and catalase (CAT) enzyme activities. The increase of Malondialdehyde (MDA), alkaline phosphatase (AKP) indicate stimulation of the immune responses to protect the liver cells from damage. The decrease in Liver TP indicated liver impairment. Decrease in Glycogen content from 6h to 96h indicated mobilization of more metabolites to cope with increased energy demand. Interestingly, results showed that heat stress trigged costly responses in the experimental sh like accelerated metabolism and deplete energy reserves, which could indirectly affect ability of sh to set up efficient long term defense responses against stress. These results provide insight into prevention and management of stress in juvenile hybrid yellow cat sh.

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Effect of dietary eicosapentaenoic and docosahexaenoic acid on expression of rat liver genes controlling cholesterol homeostasis and on plasma cholesterol level

Cited by 12 publications

References 31 publications

Tissue fatty acid deposition, plasma lipid and cytokine profile in pigs fed a diet with fish oil or palm oil

Tissue fatty acid deposition, plasma lipid and cytokine profile in pigs fed a diet with fish oil or palm oil

The effect of dietary fatty acid composition on the hepatic fatty acid content and plasma lipid profile in rats

Effects of acute heat stress on haemato-biochemical parameters, oxidative resistance ability, and immune responses of hybrid yellow catfish (pelteobagrus fulvidraco × P. vachelli) juveniles

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