Effect of Dietary Ascorbic Acid Intake on Tissue Vitamin C in Mice

Abstract

The effect of graded levels of dietary ascorbic acid on blood and tissue ascorbic acid levels in mice has been studied. Six levels of dietary ascorbic acid (0, 0.076, 0.5, 1, 5 and 8%) were used. Plasma ascorbic acid rose as dietary ascorbic acid intake increased from 1 to 8%. Mice fed a diet with 5 or 8% added ascorbic acid had significantly higher levels of ascorbic acid in the heart, kidney, lung, muscle and spleen than did control mice fed an ascorbic acid-free diet. Mice fed a diet with 1% added ascorbic acid had elevated ascorbic acid levels in the heart, kidney, lung and spleen. No significant change was observed in ascorbic acid level in the brain, adrenal gland or leukocytes in any of the experimental groups. Ascorbic acid level in the eyes was only slightly higher in mice fed a diet containing 8% added ascorbic acid than in control mice. The observation that the kidney had the greatest increase in ascorbic acid content suggests that the kidney may be a very important organ not only in elimination but also in catabolism of this vitamin. A diet containing 0.5 or 0.076% added ascorbic acid did not significantly increase ascorbic acid content in any of the organs studied. Mice fed a diet with 0.076% added ascorbic acid had slightly, but statistically significantly, lower levels of ascorbic acid in the liver, lung, muscle and spleen that control mice. Mice fed a diet with 0.5% added ascorbic acid had a lower ascorbic acid content in the liver and muscle than the controls.(ABSTRACT TRUNCATED AT 250 WORDS)

Effect of Ascorbic Acid Supplementation on Tissue Ascorbic Acid Status and Nucleic Acid Content of Young and Aged Rats.

“…In the present investigation the ascorbic acid content of liver, spleen, and kidney was also found to be low in aged rats, the content increased significantly after supplementation of the animals with ascorbic acid. These data are in conformity with a study wherein the ascorbic acid content of liver, spleen, and kidney was increased after ascorbic acid supplementation in mice [15].…”

Ascorbate Induces Ten-Eleven Translocation (Tet) Methylcytosine Dioxygenase-mediated Generation of 5-Hydroxymethylcytosine

“…The effect of ascorbate on 5-hmC appeared to reach a plateau at about 10 M in MEFs. The ascorbate concentration in normal human and mouse plasma is at the ϳ50 M range and could reach the mM range in many tissues (18), suggesting that the physiological ascorbate level is high enough to affect the generation of 5-hmC in vivo. Tet proteins are the major known enzymes to convert 5-mC to 5-hmC.…”

Effect of reactive oxygen species scavengers, antiinflammatory drugs, and calcium-channel blockers on carbon dioxide pneumoperitoneum-enhanced adhesions in a laparoscopic mouse model

“…In addition, AA 80 and 250 mg/kg IP also were effective in mice [80,81]. For the diet supplement, AA 8% was used because this concentration was shown to increase the dehydroascobate plasma concentration [82]. For dexamethasone, 2 mg/kg IP was used because 1 mg/kg was effective in rabbits [6].…”