Effect of dietary antioxidant supplementation on the oxidative status of plasma in broilers

Abstract

Summary In this study, the effect of dietary antioxidants on the plasma oxidative status of growing birds fed a diet rich in polyunsaturated fatty acids was investigated. One‐day‐old broilers were fed for 42 days a diet containing 4% linseed oil and supplemented with single plant extracts rich in antioxidants (natural tocopherols, rosemary, grape seed, green tea, tomato) or a combination of some of these plant extracts, in two different total doses (100 and 200 mg product/kg feed). A diet with synthetic antioxidants with and without α‐tocopheryl acetate (200 mg/kg feed) were also included. The plasma oxidative status was evaluated measuring the ferric reducing ability of plasma (FRAP), the superoxide dismutase (SOD) and glutathione peroxidase (GSH‐Px) activity. Lipid peroxidation was measured by thiobarbituric acid‐reactive substances (TBARS). No significant effect of the dietary treatments was observed for FRAP as well as for TBARS. However, diet affected GSH‐Px activity (p = 0.002) and a trend for an effect on SOD activity was observed (p = 0.084). A higher GSH‐Px activity was found for 200 mg/kg tomato extract and natural α‐tocopherol in relation to the corresponding 100 mg/kg treatment, and the lowest GSH‐Px activity was measured for the synthetic antioxidants treatment. The lowest and highest SOD activity were found for the 200. and 100 mg/kg treatment with tomato extract respectively. In conclusion, the oxidative status and lipid oxidation of plasma in broilers was not affected by feeding natural antioxidant extracts at the doses in the present study, but some changes in antioxidant enzyme activities were observed, of which the implication remains to be elucidated.

Antioxidant status of blood and liver of turkeys fed diets enriched with polyunsaturated fatty acids and fruit pomaces as a source of polyphenols

“…Jiang et al (2007) reported that dietary isoflavones from soybean increase SOD activity and decreased plasma TBARS levels in broiler chickens, whereas other authors demonstrated that dietary supplementation with purified flavonoids increased only blood vitamin E concentrations in Japanese quails (Sahin et al 2010). In another experiment, an increase in GSH-Px activity and a tendency towards higher levels of SOD activity were not accompanied by changes in the FRAP or TBARS values in the blood of broilers (Vossen et al 2011). …”

“…Jiang et al (2007) reported a dose-dependent increase in plasma total antioxidant activity of broilers whose diets were supplemented with 10 to 80 mg/kg of soy flavonoids. An improvement in plasma antioxidant parameters, including total antioxidant activity and MDA concentrations, was reported when broiler diets were supplemented with grape-derived resveratrol (Sahin et al 2010), rosemary, green tea and grape seed extracts (Vossen et al 2011), and purified genistein and hesperidin (Kamboh and Zhu 2013). Similar results were noted in our study where the addition of fruit pomaces, in particular seedless strawberry pomace, improved selected parameters of antioxidant status in the blood plasma, including an increase in vitamin C levels, catalase activity, capacity of hydrophilic and lipophilic fractions, and FRAP values, and a decrease in plasma peroxide levels and TBARS values in turkey livers.…”

The Fatty Acid Profile and Oxidative Stability of Meat from Turkeys Fed Diets Enriched with n-3 Polyunsaturated Fatty Acids and Dried Fruit Pomaces as a Source of Polyphenols

“…The addition of grapeseed extract produced antioxidant effects in broiler diets and excreta [12]. In another experiment, grapeseed, rosemary and green tea extracts were not highly effective in inhibiting lipid oxidation in the blood of broilers [13], which could suggest that the physiological effects of polyphenols are influenced by their source, method of acquisition and concentrations. The extraction of pure polyphenols requires complex methods involving alcohol or acetone, which increases the costs of the extraction process.…”

Review: In vivo and postmortem effects of feed antioxidants in livestock: a review of the implications on authorization of antioxidant feed additives

“…Increased SOD and CAT activity, higher ferric reducing antioxidant power (FRAP) and significantly lower malondialdehydes (MDA) were reported in heat stress layer and breeder hens supplemented with vitamins E and C (Yardibi and Turkay, 2008;Jena et al, 2013). Conversely, feeding singly or a combination of natural antioxidant supplements of grape seed extracts, tomato extracts, rosemary extracts, green tea extracts and natural tocopherols did not affect the oxidative status and lipid oxidation of plasma in broilers (Vossen et al, 2011). Though low inclusion dose was suggested as a possible cause for the observed tenuous antioxidant effect.…”