Effect of dietary anti‐Helicobacter pylori‐urease immunoglobulin Y on Helicobacter pylori infection

Suzuki, Hidekazu; Nomura, Sadahiro; Masaoka, Tatsuhiro; Goshima, Hideo; Kamata, Noriko; Kodama, Yoshikatsu; Ishii, Hiromasa; Kitajima, Masaki; Nomoto, Kikuo; Hibi, Toshifumi

doi:10.1111/j.1365-2036.2004.02027.x

Cited by 57 publications

(72 citation statements)

References 42 publications

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“…[23] In this regard, oral administration of active antibodies specific to H. pylori may be advantageous in that recognition of H. pylori by the antibody, which would efficiently inhibit adhesion of the bacterium to human epithelial cells. [24] However, a stable and reliable H. pylori infection animal model would be necessary for evaluating vaccine efficacy and helpful for understanding the pathological mechanism of the organism.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Passive Immunization with IgY Antibodies to a 58-kDaH. pyloriAntigen on Severe Gastritis in BALB/c Mouse Model

Attallah

Abbas

Ismail

et al. 2009

Journal of Immunoassay and Immunochemistry

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Consecutive triple doses of 1 x 10(8) CFU/mL of a pathogenic H. pylori strain isolated from stomach of Egyptian patients with severe gastritis were used to establish infection in BALB/c mice model. White Leghorn hens were immunized with H. pylori whole cell lysate (HpLysate) antigen and with a highly reactive 58-kDa H. pylori (Hp58) antigen. Two months later, IgY antibodies (IgY-HpLysate & IgY-Hp58) were purified from egg yolk and its efficacy was evaluated in the adopted model. Microbiological culture and immunohistochemical staining revealed that H. pylori infection was inhibited 1 week after oral passive immunization in 70% of infected BALB/c mice with a significant decrease (p < 0.05) in the degrees of gastritis. In conclusion, we have adapted BALB/c mice model for human H. pylori pathogenic strain and oral passive immunization with specific IgY antibodies to the 58-kDa antigen inhibited active H. pylori infection and decreased gastritis.

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Section: Discussionmentioning

confidence: 99%

Efficacy of Passive Immunization with IgY Antibodies to a 58-kDaH. pyloriAntigen on Severe Gastritis in BALB/c Mouse Model

Attallah

Abbas

Ismail

et al. 2009

Journal of Immunoassay and Immunochemistry

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“…[10][11][12] Peroral administration with IgY is an attractive approach because IgY does not activate mammalian complement or interact with mammalian Fc receptors that could mediate inflammatory response in the gastrointestinal tract. 13 In recent papers we have reported that anti-gingipain IgY had preventive effect against periodontitis in human patients.…”

Section: Introductionmentioning

confidence: 99%

Effect of passive immunization by anti-gingipain IgY on periodontal health of dogs

Shofiqur

Ibrahim

Isoda³

et al. 2011

Vet Sci Develop

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Anti-gingipain IgY (IgY-GP), known as hyperimmune γ-livetin from egg yolk, inhibits the enzyme activity, growth and adherence of Porphyromonas gingivalis to gingival epithelial cells. Our objective was to evaluate the efficacy of IgY-GP on periodontal health of dogs. IgY-GP was prepared from the egg yolk of hens immunized with the gingipain from Porphyromonas gingivalis ATCC 33277. Two in vivo trial models were conducted on 15 adult dogs with periodontitis by giving IgY-GP-supplemented dog feed for 8 weeks and direct application of the IgY in dental ointment to the periodontal pockets at weekly interval for 4 weeks. Clinical parameters including gingivitis, periodontitis, oral health index, bleeding on probe (BOP), pocket depth (PD), and dental calculus removal pattern for selected premolar teeth were recorded at baseline, 4 and 8 weeks post treatment.IgY-GP showed strong crossreactivity with gingipain from Porphyromonas gulae and inhibited the enzyme activity in vitro. In the dog trials, IgY-GP resulted in significant improvement of oral health parameters including gingivitis and periodontitis scores, BOP, dental calculus removal. No adverse events during and after antibody applications were noted. Oral immunotherapy by using IgY-GP is a new promising alternative to conventional preventive and therapeutic methods to improve oral health status in dogs.

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“…Oral administration of IgY antibodies has been tested for many years with promising results [80] to different pathogens as human rotavirus [81]; dental plaque formation by Streptococcus mutans [82,83]; enteropathogenic E. coli [84]; Helicobacter pylori [85,86]; Cryptosporidium parvum [87,88]; canine parvovirus [89]; Porphyromonas gingivalis [90]; Pseudomonas aeruginosa [91]; shrimp's white spot syndrome virus [92]; Eimeria acervulina [93]; E. tenella and E. maxima [94,95]; H5N1 e H1N1 in mice [96]; Vibrio cholerae [97]; rotavirus and norovirus [98]; Campylobacter jejuni [99][100][101]; and botulinum neurotoxins [102]. Immunotherapy as a passive immunization method to neutralize venom using purified IgY proved to be efficient for therapy protocol [103][104][105][106][107].…”

Section: Using Igy For Passive Immunizationmentioning

confidence: 99%

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

Júnior¹,

Santos²,

Bassi³

et al. 2017

Toxoplasmosis

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In this chapter, we describe relevant aspects of immunoglobulin Y (IgY) technology for Toxoplasma gondii applications, including comparison of avian IgY antibody with mammalian IgG antibody, egg yolk IgY production and isolation procedures, important applications for IgY antibody, and state of the art and perspectives for IgY-technology in T. gondii studies. T. gondii is a worldwide public health problem. IgY-technology provides an alternative antibody (IgY) to mammalian Immunoglobulin G (IgG) antibody. IgY-technology involves the chicken immunization, yolk IgY isolation, antibody characterization, and purified IgY application to several kinds of methods. Immunized chicken transfers a specific IgY from blood to egg yolk. Phylogenetic distance between chickens and mammals influences the generation of antibody repertoires recognizing an antigen profile. IgY is not bound to rheumatoid factor or mammalian complement protein and thus avoids the false-positive results. Yolk IgY isolation is carried out by simple procedures that are accessible for any laboratory and, also, for IgY isolation at large-scale production. IgY-technology provides antibodies for proteomic studies, diagnostic assays, and immunotherapy. Although IgY-technology is promising, there is a reduced number of investigations with IgY and T. gondii. Future perspectives involve the use of IgY-technology for the screening of new T. gondii antigens for diagnostics, therapy, or vaccine, development of innovative techniques for toxoplasmosis diagnostics and may be an immunotherapy for toxoplasmosis.

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Effect of dietary anti‐Helicobacter pylori‐urease immunoglobulin Y on Helicobacter pylori infection

Cited by 57 publications

References 42 publications

Efficacy of Passive Immunization with IgY Antibodies to a 58-kDaH. pyloriAntigen on Severe Gastritis in BALB/c Mouse Model

Efficacy of Passive Immunization with IgY Antibodies to a 58-kDaH. pyloriAntigen on Severe Gastritis in BALB/c Mouse Model

Effect of passive immunization by anti-gingipain IgY on periodontal health of dogs

IgY-Technology Applied to Studies of Toxoplasma gondii Infection

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