Effect of diet supplementation with a commercial probiotic containing<i>Pediococcus acidilactici</i>(Lindner, 1887) on the expression of five quality markers in zebrafish (<i>Danio rerio</i>(Hamilton, 1822)) testis

Valcarce, David G.; Pardo, Miguel Ángel; Riesco, Marta F.; Cruz, Ziortza; Robles, Vanesa

doi:10.1111/jai.12731

Cited by 17 publications

(15 citation statements)

References 24 publications

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“…Supporting our hypothesis are some previous results from our group on the beneficial use of probiotics in the zebrafish model [26,28]. In the present study, two probiotic strains— Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347—were vehicled in maltodextrin and provided to young animals for a total period of 3.5 months (Figure 1).…”

Section: Discussionsupporting

confidence: 88%

“…This small vertebrate has been described as a versatile animal model for fertility research [16]. In light of the early evidence about the use of probiotics for infertility treatment [26,27,28] and the publication of a theory linking gut health with testicular function [29,30], we hypothesize that probiotic strains may have an effect on reproductive performance after a long exposure of ingestion in the animal production field. The microbiome (the genes and genomes of the microbiota, as well as the products of the microbiota and the host environment [31]) is crucial in several essential functions within the organism, including supporting digestion and metabolism, keeping the gut barrier integrity, producing vitamins, and maintaining the immune system [32,33].…”

Section: Discussionmentioning

confidence: 99%

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Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

et al. 2019

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The aim of the present experiment is to study the effects of oral ingestion of a mixture of two probiotic bacteria on sperm quality and progenies. Three homogeneous groups of juvenile zebrafish were created. Once having reached adulthood (3 months postfertilization; mpf), each group received different feeding regimens: a standard diet (control), a maltodextrin-supplemented diet (vehicle control), or a probiotic-supplemented diet (a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347). The feeding regime lasted 4.5 months. Growth parameters (weight and length) were determined at 3, 5, and 7.5 mpf. Sperm motility was evaluated using computer-assisted sperm analysis at 5 and 7.5 mpf. Progeny survival, hatching rate, and malformation rate were also evaluated. Results showed that probiotic-supplemented diet improved growth parameters compared with the standard diet. The highest percentage of motile spermatozoa was reported in the probiotic-fed group. Concomitantly, the percentage of fast sperm subpopulation was significantly lower in samples derived from control males. Furthermore, there was a significant difference in progeny survival between the probiotic-fed group and the control group at three developmental times (24 hours postfertilization (hpf), 5 days postfertilization (dpf) and 7 dpf). In conclusion, in zebrafish, prolonged ingestion of a mixture of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347 has positive effects on growth, sperm quality, and progeny survival.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

et al. 2019

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“…The modulation of the intestinal microbiota composition has been proposed as one of the main mechanism of probiotic activity [37]. In a previous study, our group reported that the effects of a commercial probiotic diet supplement (Bactocell ® , Lallemand Animal Nutrition S.A., Blagnac, France), containing a lactic acid bacteria strain ( Pediococcus acidilactici ) improved molecular sperm quality markers in zebrafish testicular cells after a short period (10 days) [38], providing initial data regarding the potential use of probiotic supplementation on zebrafish male reproductive performance. In the present study, the ingestion of a supplement containing probiotic strains on a single cycle spermatogenesis evidenced a positive effect of the host’s sperm quality after a single cycle of spermatogenesis in zebrafish model.…”

Section: Discussionmentioning

confidence: 99%

Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model

et al. 2019

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Infertility is a medical concern worldwide and could also have economic consequences in farmed animals. Developing an efficient diet supplement with immediate effects on sperm quality is a promising tool for human reproduction and for domesticated animal species. This study aims at elucidating the effect of a short-time probiotic supplementation consisting of a mixture of two probiotic bacteria with proven antioxidant and anti-inflammatory activities on zebrafish sperm quality and male behavior. For this purpose, three homogeneous groups of males in terms of motility (<60%) were established. The control group was fed with a normal standard diet. The other received supplements: One group (vehicle control) was fed with maltodextrin and the other received a probiotic preparation based on a mixture (1:1) of Lactobacillus rhamnosus CECT8361 and Bifidobacterium longum CECT7347. The feeding regime was 21 days corresponding with a single spermatogenesis in zebrafish. The preparation did not modify animal weight, positively affected the number of fluent males, increased sperm concentration, total motility, progressive motility, and fast spermatozoa subpopulations. Moreover, the animals fed with the supplement showed different behavior patterns compared to control groups. Our results suggest a diet-related modulation on the exploration activity indicating a lower stress-like conduct. The studied formulation described here should be considered as advantageous in male reproductive biotechnology.

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“…as probiotic in aquaculture and most of the studies have focused on P. acidilactici ; the commercial product named Bactocell. For instance, the possible effects of dietary P. acidilactici (10 6 CFU/g) was assessed on sperm quality in zebrafish (Valcarce et al, 2015 ). After 10 days treatment of zebrafish male with probiotic, remarkable up-regulation of selected genes related to male and sperm quality was noticed.…”

Section: Lab As Probioticmentioning

confidence: 99%

Lactic Acid Bacteria in Finfish—An Update

et al. 2018

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A complex and dynamic community of microorganisms, play important roles within the fish gastrointestinal (GI) tract. Of the bacteria colonizing the GI tract, are lactic acid bacteria (LAB) generally considered as favorable microorganism due to their abilities to stimulating host GI development, digestive function, mucosal tolerance, stimulating immune response, and improved disease resistance. In early finfish studies, were culture-dependent methods used to enumerate bacterial population levels within the GI tract. However, due to limitations by using culture methods, culture-independent techniques have been used during the last decade. These investigations have revealed the presence of Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Streptococcus, Carnobacterium, Weissella, and Pediococcus as indigenous species. Numerous strains of LAB isolated from finfish are able to produce antibacterial substances toward different potential fish pathogenic bacteria as well as human pathogens. LAB are revealed be the most promising bacterial genera as probiotic in aquaculture. During the decade numerous investigations are performed on evaluation of probiotic properties of different genus and species of LAB. Except limited contradictory reports, most of administered strains displayed beneficial effects on both, growth—and reproductive performance, immune responses and disease resistance of finfish. This eventually led to industrial scale up and introduction LAB-based commercial probiotics. Pathogenic LAB belonging to the genera Streptococcus, Enterococcus, Lactobacillus, Carnobacterium, and Lactococcus have been detected from ascites, kidney, liver, heart, and spleen of several finfish species. These pathogenic bacteria will be addressed in present review which includes their impacts on finfish aquaculture, possible routes for treatment. Finfish share many common structures and functions of the immune system with warm-blooded animals, although apparent differences exist. This similarity in the immune system may result in many shared LAB effects between finfish and land animals. LAB-fed fish show an increase in innate immune activities leading to disease resistances: neutrophil activity, lysozyme secretion, phagocytosis, and production of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, and TNF-α). However, some LAB strains preferentially induces IL-10 instead, a potent anti-inflammatory cytokine. These results indicate that LAB may vary in their immunological effects depending on the species and hosts. So far, the immunological studies using LAB have been focused on their effects on innate immunity. However, these studies need to be further extended by investigating their involvement in the modulation of adaptive immunity. The present review paper focuses on recent findings in the field of isolation and detection of LAB, their administration as probiotic in aquaculture and their interaction with fish immune responses. Furthermore, the mode of action of probiotics on finfish are discussed.

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Effect of diet supplementation with a commercial probiotic containingPediococcus acidilactici(Lindner, 1887) on the expression of five quality markers in zebrafish (Danio rerio(Hamilton, 1822)) testis

Cited by 17 publications

References 24 publications

Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

Long Exposure to a Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality and Progeny Survival in the Zebrafish Model

Diet Supplemented with Antioxidant and Anti-Inflammatory Probiotics Improves Sperm Quality after Only One Spermatogenic Cycle in Zebrafish Model

Lactic Acid Bacteria in Finfish—An Update

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