Effect of dibutyryl derivatives of cyclic nucleotides on total DNA and protein synthesis in rat fetal hepatocyte cultures

Gudoshnikov, V. I.; Baranova, Irina N.; Fedotov, V. P.

doi:10.1007/bf00840998

Cited by 1 publication

(2 citation statements)

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“…Our original experimental protocol was modified from the steady state determination of receptor concentrations via Western blot (11) to the measurement of biosynthesis rate by pulse labeling with [ 35 S]Met/Cys. This change in protocol allowed the reduction of 8-Br-cGMP and atrial natriuretic factor (ANF) concentrations used in the present experiments to the level previously employed by others in short term protocols in cultured or isolated hepatocytes (25)(26)(27)(28)(29). HuH-7 cells were grown to near-confluence in MEM supplemented with either 10% FBS or 10% dFBS to which 10 to 1000 M 8-Br-cGMP, 10 nM ANF, or 100 M sodium nitroprusside (SNP) (shown to produce nitric oxide (25), an activator of soluble guanylate cyclase (29)) were added (Fig.…”

Section: Short Term Cgmp-regulated Expression Of Asgr-basedmentioning

confidence: 99%

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Cytoplasmic Protein mRNA Interaction Mediates cGMP-modulated Translational Control of the Asialoglycoprotein Receptor

Stockert

Ren

1997

Journal of Biological Chemistry

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Expression of the asialoglycoprotein receptor by the human hepatocellular carcinoma cell line HuH-7 in response to intracellular cGMP concentrations was previously shown to be regulated at the translational level. In a cell-free system, initiation of asialoglycoprotein receptor mRNA translation was dependent on the presence of the 7-methylguanylate cap site and was independent of 8-bromo-cGMP levels in which the cells were grown prior to RNA isolation. Stable transfection of COS-7 cells with deletion constructs of the asialoglycoprotein receptor H2b subunit localized the cGMP-responsive cisacting element to the mRNA 5 -untranslated region (UTR). Addition of biotin (an activator of guanylate cyclase) induced the expression of ␤-galactosidase present as a chimeric plasmid containing the H2b 187-nucleotide 5 -UTR. An RNA gel retardation assay identified a 37-nucleotide cognate sequence within this 187-nucleotide region. Titration of the 5 -UTR with a cytosolic fraction isolated from HuH-7 grown in the presence or absence of 8-bromo-cGMP or biotin provided direct evidence for an RNA-binding protein responsive to intracellular levels of cGMP. Based on these findings, it seems reasonable to propose that reduction of intracellular levels of cGMP by biotin deprivation results in a negative trans-acting factor associating with the 5 -UTR of asialoglycoprotein receptor mRNAs, thereby inhibiting translation.

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Section: Short Term Cgmp-regulated Expression Of Asgr-basedmentioning

confidence: 99%

“…1A). Within 1 h of the addition of 500 M 8-Br-cGMP and activators of both the particulate (ANF) and soluble (SNP) guanylate cyclases (25)(26)(27)(28)(29), the biosynthetic rate of ASGR was increased by 6. tained in MEM supplemented with FBS or dFBS with or without 8-Br-cGMP or its inducers (Fig. 1B).…”

Section: Short Term Cgmp-regulated Expression Of Asgr-basedmentioning

confidence: 99%

Cytoplasmic Protein mRNA Interaction Mediates cGMP-modulated Translational Control of the Asialoglycoprotein Receptor

Stockert

Ren

1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Effect of dibutyryl derivatives of cyclic nucleotides on total DNA and protein synthesis in rat fetal hepatocyte cultures

Cited by 1 publication

References 7 publications

Cytoplasmic Protein mRNA Interaction Mediates cGMP-modulated Translational Control of the Asialoglycoprotein Receptor

Cytoplasmic Protein mRNA Interaction Mediates cGMP-modulated Translational Control of the Asialoglycoprotein Receptor

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