Effect of diazepam binding inhibitor (DBI) on the fluid intake, preference and the taste reactivity in mice

Manabe, Yasuko; Toyoda, Tarō; Kuroda, Kyoko; Imaizumi, Masahiro; Yamamoto, Takashi; Fushiki, Tohru

doi:10.1016/s0166-4328(01)00263-7

Cited by 17 publications

(10 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, recent data indicate that the ODN G protein-coupled receptor can also activate adenylyl cyclase (AC; Hamdi et al, 2012). ODN exerts a wide range of biological activities which are mediated either through CBR, i.e., increase of aggressiveness and anxiety (Kavaliers and Hirst, 1986; De Mateos-Verchere et al, 1998), reduction of pentobarbital-induced sleeping time and drinking (Dong et al, 1999; Manabe et al, 2001), or through a metabotropic receptor, i.e., inhibition of food intake (Do Rego et al, 2007). Similarly, at the cellular level, the diverse effects of ODN are mediated either through CBR, i.e., stimulation of glial cell and neuroblast proliferation (Gandolfo et al, 1999; Alfonso et al, 2012) and activation of neurosteroid biosynthesis (Do Rego et al, 2001), or through a metabotropic receptor, i.e., increase of intracellular calcium concentration in astrocytes (Leprince et al, 2001) and modulation of neuropeptide expression in neurons (Compère et al, 2003, 2004).…”

Section: Introductionmentioning

confidence: 99%

The stimulatory effect of the octadecaneuropeptide ODN on astroglial antioxidant enzyme systems is mediated through a GPCR

Hamdi¹,

Kaddour²,

Vaudry³

et al. 2012

Front. Endocrin.

View full text Add to dashboard Cite

Astroglial cells possess an array of cellular defense systems, including superoxide dismutase (SOD) and catalase antioxidant enzymes, to prevent damage caused by oxidative stress on the central nervous system. Astrocytes specifically synthesize and release endozepines, a family of regulatory peptides including the octadecaneuropeptide (ODN). ODN is the ligand of both central-type benzodiazepine receptors (CBR), and an adenylyl cyclase- and phospholipase C-coupled receptor. We have recently shown that ODN is a potent protective agent that prevents hydrogen peroxide (H2O2)-induced inhibition of SOD and catalase activities and stimulation of cell apoptosis in astrocytes. The purpose of the present study was to investigate the type of receptor involved in ODN-induced inhibition of SOD and catalase in cultured rat astrocytes. We found that ODN induced a rapid stimulation of SOD and catalase gene transcription in a concentration-dependent manner. In addition, 0.1 nM ODN blocked H2O2-evoked reduction of both mRNA levels and activities of SOD and catalase. Furthermore, the inhibitory actions of ODN on the deleterious effects of H2O2 on SOD and catalase were abrogated by the metabotropic ODN receptor antagonist cyclo1-8[Dleu5]OP, but not by the CBR antagonist flumazenil. Finally, the protective action of ODN against H2O2-evoked inhibition of endogenous antioxidant systems in astrocytes was protein kinase A (PKA)-dependent, but protein kinase C-independent. Taken together, these data demonstrate for the first time that ODN, acting through its metabotropic receptor coupled to the PKA pathway, prevents oxidative stress-induced alteration of antioxidant enzyme expression and activities. The peptide ODN is thus a potential candidate for the development of specific agonists that would selectively mimic its protective activity.

show abstract

Section: Introductionmentioning

confidence: 99%

The stimulatory effect of the octadecaneuropeptide ODN on astroglial antioxidant enzyme systems is mediated through a GPCR

Hamdi¹,

Kaddour²,

Vaudry³

et al. 2012

Front. Endocrin.

View full text Add to dashboard Cite

show abstract

“…1998), shortens pentobarbital‐induced sleeping time (Dong et al. 1999) and reduces drinking (Manabe et al. 2001) in rodents.…”

mentioning

confidence: 99%

Protective effect of the octadecaneuropeptide on hydrogen peroxide‐induced oxidative stress and cell death in cultured rat astrocytes

Hamdi

Masmoudi-Kouki

Kaddour

et al. 2011

Journal of Neurochemistry

View full text Add to dashboard Cite

J. Neurochem. (2011) 118, 416–428. Abstract Oxidative stress, resulting from accumulation of reactive oxygen species (ROS), plays a critical role on astrocyte death associated with neurodegenerative diseases. Astroglial cells produce endozepines, a family of biologically active peptides that have been implicated in cell protection. Thus, the purpose of the present study was to investigate the potential protective effect of one of the endozepines, the octadecaneuropeptide ODN, on hydrogen peroxide (H2O2)‐induced oxidative stress and cell death in rat astrocytes. Incubation of cultured astrocytes with graded concentrations of H2O2 for 1 h provoked a dose‐dependent reduction of the number of living cells as evaluated by lactate dehydrogenase assay. The cytotoxic effect of H2O2 was associated with morphological modifications that were characteristic of apoptotic cell death. H2O2‐treated cells exhibited high level of ROS associated with a reduction of both superoxide dismutases (SOD) and catalase activities. Pre‐treatment of astrocytes with low concentrations of ODN dose‐dependently prevented cell death induced by H2O2. This effect was accompanied by a marked attenuation of ROS accumulation, reduction of mitochondrial membrane potential and activation of caspase 3 activity. ODN stimulated SOD and catalase activities in a concentration‐dependent manner, and blocked H2O2‐evoked inhibition of SOD and catalase activities. Blockers of SOD and catalase suppressed the effect of ODN on cell survival. Taken together, these data demonstrate for the first time that ODN is a potent protective agent that prevents oxidative stress‐induced apoptotic cell death.

show abstract

“…1983). In mice intraventricular injection of ACBP alters the preference to food (Manabe et al. 2001) and also decreases the pentobarbital‐induced sleeping time (Dong et al.…”

mentioning

confidence: 99%

Acyl coenzyme A‐binding protein (ACBP) is phosphorylated and secreted by retinal Müller astrocytes following protein kinase C activation

Qian

Bilderback

Barmack

2008

Journal of Neurochemistry

View full text Add to dashboard Cite

Horizontal optokinetic stimulation of rabbit retina in vivo evokes increased expression of acyl coenzyme A‐binding protein (ACBP), also known as ‘diazepam binding inhibitor,’ from retinal Müller cells. If the expressed ACBP were also secreted by Müller cells, then stimulus‐evoked secretion of ACBP could influence the activity of GABAA receptor‐expressing retinal neurons. In this study, we examine in vitro whether ACBP is secreted by Müller glial cells and Müller‐like QNR/K2 cells following stimulation with elevated levels of KCl and phorbol myristic acetate (PMA). KCl and PMA stimulation evoked secretion of threonine‐phosphorylated ACBP. A sequence analysis of ACBP shows that it has five potential phosphorylation sites: Two threonine sites fit a protein kinase C phosphorylation pattern. Two threonine sites fit a casein kinase II (CK2) pattern. One serine site fits a CK2 pattern. As CK2 is not expressed in QNR/K2 cells, it is probable that protein kinase C accounts for the phosphorylation of ACBP in these cells and for the PMA‐evoked secretion of ACBP. Serine phosphorylation was constitutive. Horizontal optokinetic stimulation increased threonine‐phosphorylated ACBP in rabbit retina. Phosphorylation of ACBP may influence its target affinity. We used a proteolytic fragment of ACBP, octadecaneuropeptide (ODN), to investigate how threonine phosphorylation influences its affinity for GABAA receptors. Threonine‐phosphorylated ODN had a stronger affinity for GABAA receptors than did unphosphorylated ODN or unphosphorylated ACBP. We conclude that stimulus‐induced Müller cell secretion of phosphorylated ACBP could influence the GABAergic transmission in neighboring retinal neurons.

show abstract

Effect of diazepam binding inhibitor (DBI) on the fluid intake, preference and the taste reactivity in mice

Cited by 17 publications

References 32 publications

The stimulatory effect of the octadecaneuropeptide ODN on astroglial antioxidant enzyme systems is mediated through a GPCR

The stimulatory effect of the octadecaneuropeptide ODN on astroglial antioxidant enzyme systems is mediated through a GPCR

Protective effect of the octadecaneuropeptide on hydrogen peroxide‐induced oxidative stress and cell death in cultured rat astrocytes

Acyl coenzyme A‐binding protein (ACBP) is phosphorylated and secreted by retinal Müller astrocytes following protein kinase C activation

Contact Info

Product

Resources

About