Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

Moleleki, Ntsane; Wingfield, Michael J.; Wingfield, Brenda D.; Preisig, Oliver

doi:10.1007/s10658-011-9804-4

Cited by 6 publications

(3 citation statements)

References 40 publications

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“…Therefore, during the last few years, molecular markers have been developed to distinguish between Diaporthe spp. PCR-restriction fragment length polymorphisms (PCR-RFLP) allow differentiation between D. aspalathi, D. caulivora and D. longicolla according to the number and size of DNA fragments, using a combination of restriction enzymes after rDNA internal transcribed spacer (ITS) amplification (Brumer et al, 2018;Moleleki et al, 2002;Stewart, 2015). Likewise, the use of molecular analysis supported with DNA sequencing data, including multilocus phylogeny with rDNA ITS region, elongation factor 1-α (EF1-α), β-tubulin, actin and calmodulin genes, results in a reliable method to distinguish between closely related Diaporthe isolates Mena et al, 2020;Santos et al, 2011;Udayanga et al, 2014).…”

Section: Taxonomy and Molecular Characterization Ofmentioning

confidence: 99%

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

Mena,

Stewart,

Montesano

et al. 2023

Plant Pathology

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Soybean stem canker (SSC) is an important disease caused by different Diaporthe spp., including D. aspalathi, D. caulivora and D. longicolla, that leads to soybean (Glycine max) yield losses around the world. Most studies have been focused on the morphological characterization and molecular identification of Diaporthe spp. present in SSC lesions. Several soybean resistance loci to Diaporthe spp. causing SSC have been identified, although the molecular identities of the resistance genes are at present unknown. In this review, we summarize the current knowledge on SSC disease, the molecular characterization of Diaporthe spp. and their evolutionary relationships. We highlight how recent genomic and transcriptomic information is allowing significant progress in our understanding of the molecular components and mechanisms underlying Diaporthe infection strategies as well as soybean disease resistance. The information generated, combined with available resources enabling functional genomics, will contribute to the development of breeding strategies for disease resistance, leading to a more sustainable agriculture.

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Section: Taxonomy and Molecular Characterization Ofmentioning

confidence: 99%

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

Mena,

Stewart,

Montesano

et al. 2023

Plant Pathology

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“…Diaporthe RNA virus 1 (DRV1), which is a mycovirus that was isolated from Diaporthe perjuncta, was transfected into Diaporthe ambigua and Phomopsis sp. (85). Virus particles could be used to directly transfect fungi other than their original hosts.…”

Section: Host Range and Transmission Of Mycoviruses Mycoviruses May Hmentioning

confidence: 99%

New Insights into Mycoviruses and Exploration for the Biological Control of Crop Fungal Diseases

Xiè

Jiāng

2014

Annu. Rev. Phytopathol.

368

275

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Mycoviruses are viruses that infect fungi. A growing number of novel mycoviruses have expanded our knowledge of virology, particularly in taxonomy, ecology, and evolution. Recent progress in the study of mycoviruses has comprehensively improved our understanding of the properties of mycoviruses and has strengthened our confidence to explore hypovirulence-associated mycoviruses that control crop diseases. In this review, the advantages of using hypovirulence-associated mycoviruses to control crop diseases are discussed, and, as an example, the potential for Sclerotinia sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) to control the stem rot of rapeseed (Brassica napus) is also introduced. Fungal vegetative incompatibility is likely to be the key factor that limits the wide utilization of mycoviruses to control crop diseases; however, there are suggested strategies for resolving this problem.

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“…Phomopsis species are the asexual morph of Diaporthe (Diogo et al ., 2010; Huang et al ., 2013). Like other plant‐pathogenic fungi, mycoviruses in Diaporthe species have been isolated and characterized in recent years (Preisig et al ., 2000; Moleleki et al ., 2003, 2011; Koloniuk et al ., 2014; Zhang et al ., 2015; Marzano et al ., 2016; Hrabáková et al ., 2017). Among them, Diaporthe ambigua RNA virus (DaRV1), Phomopsis longicolla hypovirus 1 (PlRV1) and Diaporthe RNA virus (DaRV) are hypovirulence factors.…”

Section: Introductionmentioning

confidence: 99%

A novel hexa‐segmented dsRNA mycovirus confers hypovirulence in the phytopathogenic fungus Diaporthe pseudophoenicicola

Zhang

Liang

et al. 2022

Environmental Microbiology

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A novel hexa-segmented double-stranded RNA (dsRNA) mycovirus was isolated and characterized from the filamentous phytopathogenic fungus Diaporthe pseudophoenicicola and was named Diaporthe pseudophoenicicola chrysovirus 1 (DpCV1). The full-length cDNAs of dsRNA1-6 were 3335, 3030, 3039, 2980, 963, and 780 bp, respectively. Sequence analysis indicated the presence of nine open reading frames (ORFs) in the DpCV1 genome. ORF1 in dsRNA1 putatively encoded the RNA-dependent RNA polymerase (RdRp) and ORF3 in dsRNA2 encoded a capsid protein (CP). The seven remaining ORFs, ORF2 in dsRNA2, ORF4 in dsRNA3, ORF6, seven in dsRNA4, ORF8 in dsRNA5, and ORF9 in dsRNA6, encoded proteins with unknown functions. Phylogenetic analysis revealed that DpCV1 is closely related to members of the cluster I group within the family Chrysoviridae but formed a separate clade. Importantly, all the six segments of DpCV1 were cured successfully through single spore isolation to obtain the isogenic virus-free strains. DpCV1 can confer hypovirulence to the fungal host of Diaporthe pseudophoenicicola. Compared with the virus-free strain, WC02 harbouring the DpCV1 is more sensitive to fungicide prochloraz. Furthermore, the cell wall of DpCV1 infected strain was loose and enlarged. This is the first report of a hexa-segmented tentative chrysovirus in D. pseudophoenicicola.

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Effect of Diaporthe RNA virus 1 (DRV1) on growth and pathogenicity of different Diaporthe species

Abstract: Pathogenicity studies showed that the transfected isolates have reduced aggresiveness.

Cited by 6 publications

References 40 publications

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

New Insights into Mycoviruses and Exploration for the Biological Control of Crop Fungal Diseases

A novel hexa‐segmented dsRNA mycovirus confers hypovirulence in the phytopathogenic fungus Diaporthe pseudophoenicicola

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