Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats Through Multiple Mechanisms

Abstract

Dear Editor,We are interested in reviewing an article by Wang H et al 1 entitled "Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats through Multiple Mechanisms" recently published in the Dose-Response journal. We would like to address our concerns notably on methods implemented in this study.Whether the researchers performed pre-conditioned training in rats prior to implementing the Zea-Longa protocol should be described in detail. Since ischemia-reperfusion (IR) injury is associated with oxidative stress particularly during reperfusion, the neuroprotective effects of diammonium glycyrrhizinate (DG) might be partly due to its antioxidant properties. 2 Hence, malondialdehyde (MDA) assay or chemiluminescence methods for DG antioxidant activity measurement should be performed. 3 Moreover, other modalities such as stem cells, cell secretomes, or neurotrophic factor-mediated neuroprotection in IR injury are worth revisiting and brought to in-depth discussion. 4,5 Finally, how the authors extrapolate the optimal DG dose at 20 mg/kg/day to humans requires further explanation since the results of this study are very promising and applicable in clinical settings. Given several perspectives described above, some improvements can be made to optimize the findings of this study in further research.