2015
DOI: 10.5507/bp.2014.007
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Effect of diabetic osteoblasts on osteogenic differentiation of human umbilical cord mesenchymal stem cells

Abstract: Background. This study was aimed to investigate whether osteoblasts from diabetic patients have a promoting effect on osteogenesis of human umbilical cord mesenchymal stem cells (HUMSCs). Methods. HUMSCs were co-cultured with osteoblasts of diabetic and non-diabetic patients. Morphological appearance and cytochemical characteristics of the non-diabetic osteoblasts and diabetic osteoblasts were observed by hematoxylin-eosin staining, type I collagen protein expression, alkaline phosphatase (ALP) staining and Al… Show more

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