Effect of diabetes mellitus on levels of insulin-like growth factors and their carrier proteins in Guerin’s carcinoma and it’s perifocal tissue in rats

Frantsiyants, Elena Mikhaylovna; Бандовкина, В. А.; Kaplieva, Irina V.; Surikova, Ekaterina I.; Pogorelova, Yulia A.; Pogorelova, Yu. A.; Cheryarina, Natalia D.; Качесова, П. С.; Nemashkalova, Ludmila A.

doi:10.17709/2410-1893-2021-8-4-4

Cited by 1 publication

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the females on the background of DM, already after 10 days, the dynamics of tumor growth was opposite and the subcutaneous component of the tumor, equal to 15.98±1.6 cm 3 , was 1.5 times less (p<0.05) than in the reference group where the average volume tumor size was 23.6±2.5 cm 3 . After 15 days, the difference between the groups persisted, and the tumor volume in the main group in the females was 1.3 times less than that in the reference group (39.35±3.8 versus 50.4±5.0 cm 3 , p<0.05), however, metastatic lesions of internal organs: the ovaries, the kidneys, the parietal and visceral peritoneum were found [8].…”

Section: Resultsmentioning

confidence: 85%

“…Thus, drawing structural and functional parallels between the two simulated processes, DM and the progression of Guerin's carcinoma, one can trace the relationship of certain events at the molecular and organ levels, associated with gender characteristics. Based on the data of research works on biochemical studies [8], in female rats of the main group, during the formation of a tumor node, the perifocal zone of the tumor, which is supersaturated with glucose, is characterized by high metabolic activity, but at the same time, the content of insulin-like growth factor 1 (IGF-I) is significantly lower, than that found in the tumors. On the contrary, in the tumor node itself, even with a relatively small volume, a highly aggressive environment is formed, combining moderate glucose saturation with an excess of IGF-I, which, like "gunpowder in a barrel", explodes its protective armor and stimulates metastatic progression in a foreign territory, namely, the organs with abundant vascularization: the ovary, the kidney and the peritoneum.…”

Section: Morphological Examination Of the Peritoneummentioning

confidence: 99%

“…As to the scale of modern medical problems, such as the growth of oncological, cardiovascular diseases, the pandemic of coronovirus infection, there is a significant increase in the incidence of diabetes mellitus (DM), which is one of the most topical pathologies that entails profound metabolic shifts at different levels of a self-organization organism: from the molecular to the systemic one. The clinical course of DM is accompanied by the emergence of many risk factors: the development of nephropathy and retinopathy, the determination of multiple vascular complications, up to the coronary, carotid, and peripheral vessels [8,9]. The main pathological changes in DM affect the biochemical environment of the body and are characterized by dysregulation of energy metabolism (glucose, amino acids and fatty acids), insulin resistance, rapid development of oxidative stress, and inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…The main pathological changes in DM affect the biochemical environment of the body and are characterized by dysregulation of energy metabolism (glucose, amino acids and fatty acids), insulin resistance, rapid development of oxidative stress, and inflammation. This leads to various complications and entails functional and morphological damage to target organs, such as the heart, the kidneys, the liver, the eyes, the reproductive and other organs [8,9].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Relationship between comorbid pathology and tumor progression. Morphological portrayal of internal organs in modeling the growth of Guerin’s carcinoma under diabetic conditions

Shikhlyarova¹,

Frantsiyants²,

Kaplieva³

et al. 2022

View full text Add to dashboard Cite

Topicality. An increase in the incidence of malignant tumors progressing against the background of various comorbid pathologies determines the need to study the mutual influence of pathological processes using experimental modeling. Such models, for example, can reproduce the tumor growth modified by the comorbid condition of diabetes mellitus, the incidence rate of which is now increasing exponentially. In this case, a clear demonstration of changes in the structure of organs outside the zone of the primary tumor node, using data on the direct growth of the tumor and the content of diabetes markers therein and in the perifocal zone, can serve as an evidence-based argument for the implementation of the mechanism of modified tumor progression. The aim of our research work is to assess the state of the histological structure of some internal organs (the kidneys, the ovaries, the peritoneum) when modeling the main pathological process in animals, the growth of Guerin’s carcinoma, against the background of a comorbid state of experimental diabetes. Materials and methods. We used 32 outbred male and female rats weighing 180-220 g to reproduce the model of experimental diabetes by a single intraperitoneal injection of alloxan at a dosage of 150 mg/kg of body weight. One week after the production of persistent hyperglycemia in the range of 25.4±1.2 mmol/l, the animals were transplanted with Guerin’s carcinoma subcutaneously in the region of the right shoulder blade. Upon expiration of 2 weeks, the animals were decapitated, and the harvested organs were prepared according to the practice stages of morphological preparation for staining sections with hematoxylin-eosin, followed by morphological examination of the structure with the use of the Leica DM LS2 microscope with the Olympus optical. C-5050 Zoom video camera and the Morfotest software. Photographing was carried out with magnification x10, x40, x100. Results. Our study of the morphological portrayal of the ovary, the kidney, the visceral and parietal peritoneum bears witness to the identity of the changes, consisting in a total metastatic lesion and abnormal transformation of the normal structure of all the studied organs only in the female rats, when modeling the comorbid state of diabetes mellitus. At the same time, the aggressive nature of the tumor progression was manifested in the blood filling of the vessels and hemorrhage, followed by the release of tumor cells, their settlement, the enhanced proliferation, the formation of strands and compaction of cell aggregations throughout the volume of the organ. Some gender specific features of the tumor progression were noted, which were found in the female rats along the path of active metastasizing in case of small primary tumors, and in the male rats along the path of stimulating the growth of the primary focus without metastasizing. It was revealed that these differences are associated with different degrees of saturation of the tumor and perifocal zone with glucose, and they are determined by the state of the insulin/insulin-like growth factor (IGF) axis. Conclusion. Morphological examination of the organs affected by metastatic Guerin’s carcinoma in the female rats with diabetes mellitus makes it possible to detect not only the synergy of both pathological processes, but also a powerful pro-oncogenic effect of the comorbid state of diabetes in the implementation of the tumor growth program.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Morphological Examination Of the Peritoneummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations