Effect of di(2-ethylhexyl)phthalate (DEHP) on spermatogenesis in adult rats

Parmar, Devendra; Srivastava, S. P.; Seth, P. K.

doi:10.1016/0300-483x(86)90091-0

Cited by 48 publications

(25 citation statements)

References 27 publications

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“…Several studies indicate that phthalates can negatively affect reproductive function in laboratory animals. [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] Di-n-butyl phthalate (DnBP) and butyl benzyl phthalate (BBP), which are mainly used in printing ink, have been shown to have a very weak estrogenic activity in some in vitro studies but not in others. [38][39][40] Other more common phthalates like diethyl hexyl phthalate (DEHP), di-iso-nonyl phthalate and di-iso-decyl phthalate have been tested and found not to have any estrogenic activity.…”

Section: Introductionmentioning

confidence: 99%

General gynaecology: Association of phthalate esters with endometriosis in Indian women

Reddy¹,

Rozati²,

Reddy

et al. 2006

BJOG

169

101

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Sample Blood samples were collected from 49 infertile women with endometriosis (study group); 38 age-matched women without endometriosis (control group I) but with infertility related to tubal defects, fibroids, polycystic ovaries, idiopathic infertility and pelvic inflammatory diseases diagnosed by laparoscopy and a further group of 21 age-matched women (control group II) with proven fertility and no evidence of endometriosis and other gynaecological disorders during laparoscopic sterilisation.Methods Concentrations of PEs were measured using gas chromatography.Main outcome measures Evaluation of PEs concentrations in women with endometriosis compared with women free from the disease.Results Women with endometriosis showed significantly higher concentrations of di-n-butyl phthalate (DnBP), butyl benzyl phthalate (BBP), di-n-octyl phthalate (DnOP) and diethyl hexyl .68] micrograms/ml). The correlation between the concentrations of PEs and different severity of endometriosis was strong and statistically significant at P < 0.05 for all four compounds (DnBP: r = +0.73, P < 0.0001; BBP: r = +0.78, P < 0.0001; DnOP: r = +0.57, P < 0.0001 and DEHP: r = +0.44, P < 0.0014).Conclusions This study suggests that PEs may have an aetiological association with endometriosis.

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Section: Introductionmentioning

confidence: 99%

General gynaecology: Association of phthalate esters with endometriosis in Indian women

Reddy¹,

Rozati²,

Reddy

et al. 2006

BJOG

169

101

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“…Bei der F1-Generation, die analoge Dosen DBP verabreicht bekam, fanden sich eine Reduktion des Hodengewichts, Kryptorchismus, verminderte Spermatozoendichten und Malformationen des Nebenhodens und des Penis [32]. Die DEHP-Exposition adulten Ratten führt zur Abnahme der epididymalen Spermatozoendichte unter Modulation von Enzymen, die die Spermatozoenreifung im Nebenhoden fördern [19]. Der exakte Mechanismus, über den die Phthalate ihre Reproduktionstoxizität bewirken, muss noch geklärt werden.…”

Section: Phthalateunclassified

“…Die Veränderungen umfassten reduzierte testikuläre Spermatozoendichten (Maus), reduziertes Gewicht der ventralen Prostata (Ratte) und der Bläschendrüsen (Ratte, Maus) [2]. Weiterhin konnte gezeigt werden, dass non-ortho (dioxinartige) PCB und 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) die Spermatogenese der Ratte und des MarmosetAffen (Callithrix jacchus) in vitro und vivo beeinträchtigen [19,24,25]. Insbesondere die Sertolizellen und die frühen Spermatiden zeigten morphologische Veränderungen [19,24].…”

Section: Chlorierte Kohlenwasserstoffeunclassified

“…Weiterhin konnte gezeigt werden, dass non-ortho (dioxinartige) PCB und 2,3,7,8-Tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) die Spermatogenese der Ratte und des MarmosetAffen (Callithrix jacchus) in vitro und vivo beeinträchtigen [19,24,25]. Insbesondere die Sertolizellen und die frühen Spermatiden zeigten morphologische Veränderungen [19,24]. Raychoudhury et al [23] schließen aus ihren In-vitroStudien an isolierten Serolizellen der Ratte, dass die toxischen Effekte von PCB 77 auf eine direkte und nicht auf eine estrogenabhängige Wirkungen zurückzuführen sind.…”

Section: Chlorierte Kohlenwasserstoffeunclassified

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Beeinflussung testikulärer Zellen durch endokrin aktive Umweltchemikalien

Pflieger-Bruss¹,

Schill²

2002

Reproduktionsmedizin

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“…In this study the permanent changes in adult Sertoli cell vimentin distribution after repeated dosing of DEHP, and the hypothesised interaction with acetone were studied. Few data on male fertility after short-term exposure to DEHP in rats have been published (Parmar et al 1986). Therefore male fertility after 4 weeks of dosing was investigated by mating dosed males with undosed females.…”

mentioning

confidence: 99%

Toxicity Study of Di(2‐Ethylhexyl)phthalate (DEHP) in Combination with Acetone in Rats

Dalgaard¹,

Østergaard

Lam

et al. 2000

Pharmacology & Toxicology

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Abstract:In two separate studies with exposure duration 9 weeks or 4 weeks, male Wistar rats were dosed with di(2-ethylhexyl)phthalate (DEHP) by gavage and exposed to drinking water with or without acetone (0.5% wt/v in the 9-week study, 1.0% wt/v in the 4-week study). In the 9-week study the doses of DEHP were 0, 125, 250, 500 or 1000 mg/kg b.wt. In the 4-week study the doses of DEHP were increased to 1000, 5000 and 10,000 mg/kg b.wt. In the 9-week study, the relative liver weight was increased in the rats exposed to 500 and 1000 mg/kg b.wt. No interaction of DEHP and acetone was observed in any of the measured parameters. In the 4-week study DEHP, at the highest dose level, resulted in severe general toxicity. The group exposed to DEHP in combination with acetone was more affected. Male fertility was decreased. Body weight was decreased, and the relative weight of the liver, kidney, heart, brain and adrenals increased. The relative weight of the testes decreased in the 5000 and 10,000 mg/kg b.wt. groups. The weight of seminal vesicles and epididymals decreased at 10,000 mg/kg b.wt. In animals exposed to 5000 and 10,000 mg DEHP/kg b.wt. a severe atrophy of the seminiferous tubules and a slight diffuse Leydig's cell hyperplasia was observed. The cellular debris and conglomerates of desquamated cells found in the lumen of the seminiferous tubules were immunostained positive for vimentin. This indicates that Sertoli cell cytoplasm is included in the conglomerates -an interesting finding not previously described. No specific interaction of DEHP and acetone was observed in any of the measured parameters.It is well known that di(2-ethylhexyl)phthalate (DEHP) induces testicular atrophy in rats (Woodward 1988). Low doses of the monoester derivative mono(2-ethylhexyl)-phthalate (MEHP) have been shown to induce the same effect as the diester, and MEHP is considered the most potent metabolite of DEHP (Teirlynck et al. 1988). The role of other metabolites, e.g. 2-ethylhexanol is not fully elucidated. Both the phthalic acid and the aliphatic (ethylhexyl) part of DEHP may be toxic to the testis. The possible contribution of the aliphatic part to the testis toxicity can be examined by administering acetone concomitantly with DEHP. If enhancement of testis toxicity occurs, this may be interpreted as an indication of ethylhexyl contribution to toxicity. A small fraction of 2-ethylhexanol may be metabolised to a g-diketone, and it is well known that acetone potentiates the toxicity of g-diketones (Misumi & Nagano 1985). We have previously reported on the interaction between acetone and 2,5-hexanedione, the testis toxic metabolite of n-hexane (Larsen et al. 1991). The present study was performed to investigate a possible enhancement of the toxicity of DEHP by acetone in testis and the peripheral nervous system. Several Sertoli cell toxicants i.e. acrylamide, carbon disulfide, 2,5-hexanedione and tri-o-cresyl phosphate induce testis toxicity and peripheral neuropathy, possibly by affecting the cytoskeleton (Boekelheide 1993 the ne...

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Effect of di(2-ethylhexyl)phthalate (DEHP) on spermatogenesis in adult rats

Cited by 48 publications

References 27 publications

General gynaecology: Association of phthalate esters with endometriosis in Indian women

General gynaecology: Association of phthalate esters with endometriosis in Indian women

Beeinflussung testikulärer Zellen durch endokrin aktive Umweltchemikalien

Toxicity Study of Di(2‐Ethylhexyl)phthalate (DEHP) in Combination with Acetone in Rats

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