Proteins
are commonly encapsulated in alginate gels for
drug delivery
and tissue-engineering applications. However, there is limited knowledge
of how encapsulation impacts intrinsic protein properties such as
folding stability or unfolding kinetics. Here, we use fast relaxation
imaging (FReI) to image protein unfolding in situ in alginate hydrogels
after applying a temperature jump. Based on changes in the Förster
resonance energy transfer (FRET) response of FRET-labeled phosphoglycerate
kinase (PGK), we report the quantitative impact of multiple alginate
hydrogel concentrations on protein stability and folding dynamics.
The gels stabilize PGK by increasing its melting temperature up to
18.4 °C, and the stabilization follows a nonmonotonic dependence
on the alginate density. In situ kinetic measurements also reveal
that PGK deviates more from two-state folding behavior in denser
gels and that the gel decreases the unfolding rate and accelerates
the folding rate of PGK, compared to buffer. Phi-value analysis suggests
that the folding transition state of an encapsulated protein is structurally
similar to that of folded protein. This work reveals both beneficial
and negative impacts of gel encapsulation on protein folding, as well
as potential mechanisms contributing to altered stability.