Effect of developmental stage on arginase and urea production in the liver of rat

Paik, Woon Ki; Lew, Betty; Farooqui, Jamal; Kim, Sangduk

doi:10.1016/0006-2944(84)90091-7

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The activity of arginase was determined in cytosolic liver extracts by measuring the amount of urea produced in reaction samples using the method Paik et al (1984) with some modifications. The samples were prepared in 1.5 mL of 0.2 M glycine buffer (pH 9.5) containing 0.5 mL liver cytosolic extract, 0.2 mL 5 μM MnCl 2 , and 0.4 mL of 50 μM L-arginine.…”

Section: Ethicsmentioning

confidence: 99%

Nicotinamide prevention in diabetes-induced alterations in the rat liver

Kuchmerovska,

Yanitska,

Horkunenko

et al. 2023

Endocrine Regulations

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Objective. The study was performed to elucidate whether nicotinamide (NAm) can attenuate the diabetes-induced liver damage by correction of ammonia detoxifying function and disbalance of NAD-dependent processes in diabetic rats. Methods. After four weeks of streptozotocin-induced diabetes, Wistar male rats were treated for two weeks with or without NAm. Urea concentration, arginase, and glutamine synthetase activities, NAD+ levels, and NAD+/NADH ratio were measured in cytosolic liver extracts. Expression of parp-1 gene in the liver was estimated by quantitative polymerase chain reaction and PARP-1 cleavage evaluated by Western blotting. Results. Despite the blood plasma lipid peroxidation products in diabetic rats were increased by 60%, the activity of superoxide dismutase (SOD) was reduced. NAm attenuated the oxidative stress, but did not affect the enzyme activity in diabetic rats. In liver of the diabetic rats, urea concentration and arginase activity were significantly higher than in the controls. The glutamine synthetase activity was decreased. Decline in NAD+ level and cytosolic NAD+/NADH ratio in the liver of diabetic rats was observed. Western blot analysis demonstrated a significant up-regulation of PARP-1 expression accompanied by the enzyme cleavage in the diabetic rat liver. However, no correlation was seen between mRNA expression of parp-1 gene and PARP-1 protein in the liver of diabetic rats. NAm markedly attenuated PARP-1 cleavage induced by diabetes, but did not affect the parp-1 gene expression. Conclusions. NAm counteracts diabetes-induced impairments in the rat liver through improvement of its detoxifying function, partial restoration of oxidative stress, NAD+ level, normalization of redox state of free cytosolic NAD+/NADH-couples, and prevention of PARP-1 cleavage.

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Section: Ethicsmentioning

confidence: 99%

Nicotinamide prevention in diabetes-induced alterations in the rat liver

Kuchmerovska,

Yanitska,

Horkunenko

et al. 2023

Endocrine Regulations

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In vitro kinetics of the mouse hepatic arginase inhibition by hexachlorophene

Prasad

Rajendra

Indira

1987

Pesticide Biochemistry and Physiology

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Purification and Kinetic Properties ofMus Booduga(Gray) Hepatic Arginase

Prasad

Valluru

Sreekanth

et al. 1997

Journal of Enzyme Inhibition

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Hepatic L-arginase from the Mus booduga (Gray) was purified and its kinetic characteristics were investigated. The enzyme was not adsorbed on DEAE-cellulose, but was retained on CM-cellulose column at pH 7.2. The Michaelis-Menten constant was 8.3 mM for L-arginine and was independent of pH in the range of 7.5-10.5. L-arginine concentrations as high as 0.4 M did not exert substrate inhibition in the pH range 7.4-10.0. Manganese was required at a concentration of 0.05 M for full activation of the enzyme. L-ornithine and L-lysine inhibited the enzyme competitively with inhibitory constants of 1.9 mM and 3.7 mM respectively. Several properties of the L-arginase from Mus booduga clearly identify it as an enzyme similar to ureotelic basic arginases from mammalian liver.

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Effect of developmental stage on arginase and urea production in the liver of rat

Cited by 3 publications

References 12 publications

Nicotinamide prevention in diabetes-induced alterations in the rat liver

Nicotinamide prevention in diabetes-induced alterations in the rat liver

In vitro kinetics of the mouse hepatic arginase inhibition by hexachlorophene

Purification and Kinetic Properties ofMus Booduga(Gray) Hepatic Arginase

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