Effect of deslorelin on the induction of estrus in anestrous bitches

Abstract

The efficacy of one or multiple doses of an injectable formulation of deslorelin (a GnRH agonist) was evaluated to induce estrus in anestrous bitches. Thirteen animals composed three groups: group 1 (n=5, single IM injection of 2mg deslorelin), group 2 (n=5, four IM injections of 2mg deslorelin in alternate days), and control group (n=3, four IM saline injections in alternate days). Daily clinical evaluations, sexual behavior, vaginal cytology, plasma progesterone concentration, ovaryhysterectomy and macroscopic evaluation of the uterus and ovaries were done. In group 1, none of the bitches showed signs of estrus, while two developed clinical signs and vaginal cytology of proestrus. In group 2, all animals presented proestrus, four presented estrus, and three ovulated; resulting in a functional corpus luteum and high progesterone concentration until day 25 of diestrus, when ovaryhysterectomy was performed. The duration of the stages of deslorelin induced cycles and the progesterone profile were similar to those described in the literature, and no side effects were observed. In conclusion, injectable formulation of deslorelin in multiple injections was effective to induce fertile estrus in anestrous bitches. Keywords: bitch, deslorelin, induction of estrus RESUMO Avaliou-se a eficácia de uma formulação injetável de deslorelina (agonista do GnRH) na indução de estro em cadelas no anestro e seus efeitos no aparelho genital, com uma ou múltiplas aplicações. Treze animais compuseram os grupos: grupo 1 (n=5, 2mg de deslorelina via IM), grupo 2 (n=5, quatro injeções IM de 2mg de deslorelina em dias alternados) e grupo-controle (n=3, quatro injeções IM de solução salina em dias alternados). Foram realizadas avaliações clínicas diárias do comportamento

Clinical and Endocrine Short-Term Effects of GnRH Analogue Deslorelin in Prepubertal Bitches: Does A “Flare-up” Occur?

“…In disagreement with these results, in the present study, we observed an increase in the average rate of 15-20% of SCI accompanied by a moderate increase in serum E2 concentrations in four of the nine treated bitches during the first eight weeks after the implant placement. Similar to our findings, Lanna et al 23 reported an increase in the proportion of superficial cells, reaching a high average of 44±15% without clinical signs of estrus in adult bitches received a single intramuscular injection of 2 mg deslorelin. In our study, dogs in the control group (G3) did not show an increase in SCI or P4 and E2 concentrations which comparable to anestrus during the observation period.…”

Evaluation of prostaglandin F2a, estradiol benzoate and deslorelin acetate protocol for oestrus induction in bitches

“…The progressive sP4 increment, indirectly suggests that luteinization occurs in response to LH amplitude and that frequency pulses rise. On day 10.2±2.8, all bitches reached sP4 levels of 1.5-2.5 ng/mL, in agreement with reports using others GnRH agonists or deslorelin (Concannon et al 2006, Lanna et al 2010, Fontaine et al 2011, Walter et al 2011. sP4 declined to < 1 ng/mL after artificial insemination in 100% of bitches, indicating no corpora lutea formation and ovulation failure.…”

“…It also presents the disadvantage of having to perform an incision in the skin to remove the implant. One study (Lanna et al 2010) using a slow-release injectable form of DA 2 achieved 100% of estrus induction, to 2 mg im/48 h four times, without producing unwanted side effects at the site of injection. Concannon et al (2006) have used another GnRH agonist (lutrelin) with the same power as DA, proposed a dose of 0.6 μg/k (0.2-1.2 μg/k) with an efficiency of 92% in oestrus induction and 100% pregnancy rate, suggesting that this same DA dose can be used, to obtain similar results for a oestrus induction protocol in dogs.…”

Changes in serum progesteron and 17beta-estradiol concentration after application of Suprelorin (4,7 deslorelin acetate) implant for estrus induction in bitches

“…На противагу цьому Arlt et al (2015) повідомляє про появу кіст після застосування імпланту Супрелорін, а Borges et al (2015) вказує про появу кіст у поодиноких сук після декількаразового застосування імпланту. Lanna et al (2010) зазначає, що при застосування деслореліну в ін'єкційній формі 3/10 сук, які отримували препарат, відчували біль і отримали легкий набряк після в/м ін'єкцій. Наші результати вказують на те, що при застосуванні імпланту Супрелорін не спостерігалось жодних місцевих ускладнень чи негативних ефектів від підшкірного застосування імплантату, не було негативних наслідків у сук у вигляді кіст яєчників чи піометри, статевий цикл проходив схоже з природною тічкою і суки почувались добре.…”