Effect of dentinal tubule occlusion by dentifrice containing nano‐carbonate apatite

Abstract

This study evaluated the effects of the short-term use of a dentifrice containing nano-sized carbonate apatite (n-CAP) on the occlusion of the dentinal tubules using a scanning electron microscope (SEM) and an image analyser in vitro. One hundred human dentine specimens were wet ground with a silicone carbide papers and etched with 6% citric acid for 1 min to allow complete opening of the dentinal tubule. Specimens showing complete opening tubules were used as the baseline. The specimens were divided randomly into five groups: G1: 0% n-CAP, G2: 5% n-CAP, G3: 10% n-CAP, G4: 20% n-CAP and G5: 10%strontium chloride (SrCl(2)). Five specimens from each group were brushed by applying 50, 100, 250 and 500 strokes, respectively. All the specimens were evaluated by a SEM (x3000), and the degree of occlusion of the dentinal tubules was quantified using an image analyser. The results were analysed by one-way anova and a Tukey's test using the spss 12.0 statistical package program. The dentifrice containing 20% n-CAP for 50 strokes, which indicated 2-day use, showed the highest tubular occlusion than the other groups (P < 0.05). Moreover, this group showed 79.5% and 77.4% less open tubular area than the baseline and 0% n-CAP group, respectively. The groups containing various concentrations of n-CAP showed significant differences in the SrCl(2) group after tooth-brushing for 500 strokes, which indicated 17-day use. According to this examination of the short-term use of desensitizing dentifrices in vitro, the dentifrice containing 20% n-CAP was the most effective in occluding the dentinal tubules.

Effect of mesoporous silica nanoparticles on dentinal tubule occlusion: An in vitro study using SEM and image analysis

“…Therefore, when mixed with distilled water, a large quantity of instantly formed MSNs easily entered the dentinal tubules and deposited on the walls of dentinal tubules. SEM observation results and statistical image analysis proved that MSNs not only almost completely occluded the dentinal tubules at the dentin surface, but also penetrated the dentinal tubules to a depth of about 105 μm, presenting results which were superior to the previous work [11][12][13] . The tubular occlusion efficacy of Ca 2+ / PO4 3− @MSNs group was similar to that of MSNs group at both the dentin surface and in the depth of dentinal tubules.…”

“…Length of the precipitate formed along the dentinal tubules from the dentinal surface to the bottom of precipitate was measured in at least 20 dentinal tubules for each sample. In addition, the degree of occlusion of the dentinal tubules on the dentinal surface was quantified using SEM and image analyses (Image-Pro PLUS 6.0, Media Cybernetics, Silver Spring, MD, USA) 13,20) . Three images were taken at a constant magnification of ×1000, with a 50-μm scale bar from the central portion of each sample (Schematic 1C).…”

“…The novel MSNs-based nanomaterials showed great potential as a treatment option for dentin hypersensitivity. on irregular spaces 13) . Inspired by the properties of CPP, silica, and nanomaterials, we introduced novel mesoporous silica nanoparticles (MSNs) for dentinal tubule occlusion.…”

“…They include light-cured bonding materials 5) , strontium chloride, sodium fluoride 6) , potassium nitrate and potassium oxalate 7) , silica 8) , calcium-based remineralizing agents [9][10][11][12] , and nanosized carbonate apatite 13) . Some dentin hypersensitivity treatment products contain a variety of chemical agents.…”

Occluding Effect of the Application of Fluoride Compounds and Desensitizers on Dentinal Tubules

“…In addition, Lee et al 34) reported that the dentinal tubule size increased as the exposure time of the dentin to the desensitizing toothpaste increased based on the experiment results regarding the deposited dentin, wherein a toothpaste containing nano-sized carbonate apatite was applied in a decalcification solution. It was found that the efficient time of the active ingredients of desensitizing agents was affected by the frequency of the brush strokes and the exposure time to the acid.…”