Effect of Dentin Pretreatment with Arginine on Microshear Bond Strength of Etch-and-Rinse or Self-Etch Adhesive Systems

Abstract

Objective The main purpose of this study was to evaluate the effect of dentin pretreatment with 8% arginine on the immediate and 6-month bond strength (BS) of adhesive systems and surface morphology of dentin. Materials and Methods Dentin bovine specimens (5 × 5 mm) were allocated into following pretreatments: without pretreatment (conventional technique, C) or exposure to arginine solution (A) for 5 minutes prior to tooth restoration. The adhesive procedure was performed using (n = 12): 3-step etch-and-rinse adhesive (Scotchbond Multipurpose—SBMP [C+SBMP and A+SBMP]); 2-step etch-and-rinse adhesive (Single Bond 2 - SB [C+SB and A+SB]); or 2-step self-etch adhesive (Clearfil SE Bond - CSEB [C+CSEB and A+CSEB]). After 24 hours, the composite resin restorations were evaluated immediately and after 6 months of water storage using a microshear test and fracture pattern. The dentin surface exposed to A was assessed by scanning electron microscopy (SEM). The immediate BS data were submitted to a two-way analysis of variance and Tukey's test, and the long-term BS results were analyzed using Kruskal–Wallis and Dunn tests (α = 0.05). Results There was a significant decrease in immediate and 6-month BS for the A+SB and A+CSEB groups, which differed statistically from the C group. The fracture pattern was predominantly adhesive for SB and CSEB adhesive for C and A. The SEM images presented a different conditioning pattern of the dentin exposed to A. Conclusion The dentin pretreatment with arginine interfered negatively in the immediate and long-term BS of the simplified adhesive system. However, the SBMP adhesive was not affected by arginine pretreatment presenting the most satisfactory results.

The Effect of a Resin-Based Desensitizer Containing Glutaraldehyde on Cervical Dentin Hypersensitivity: A Randomized Controlled Clinical Trial

“…Recently new products have been recommended for their desensitizing effect. The results of several topical desensitizing medications, however, have reportedly been transitory because they do not attach to the dentin surface (10). Studies have revealed that although the first therapy of choice is to apply desensitizer solutions and dentifrices containing ferric aluminium and potassium oxalates, their effects are transient.…”

Comparative Evaluation of Resin Dentin Interface using Universal and Total- Etch Adhesive Systems on Sound and Eroded Dentin: In Vitro Study

“…Gabriela et al reported that arginine dentine pretreatment had positive effect on adhesion especially with self-etch adhesive system. 16 From the reported studies, it was observed that eroded dentin exhibited lower bond strength compared with sound dentin, but this might also be affected by the age factor. A clinical based study conducted by Federlin et al has showed that class V restorations bonded to the unprepared surface of eroded dentin for a period of 12 months, displayed good retention regardless of the type of restoration.…”

“…Gabriela et al reported that arginine dentine pretreatment had positive effect on adhesion especially with self-etch adhesive system. 16 …”

Aging Methods—An Evaluation of Their Influence on Bond Strength

“…Human enamel and dentin tissues present clear differences regarding their micromorphology and the balance of organic and inorganic contents, which consequently result in different performance of adhesive systems onto such tissues. 1,2 The interface between the resin composite and the cavity walls is the most critical region in bonded restorations. Unprotected collagen depleted fibrils 3 and chemical hydrolysis of the ester bonds are considered the main reasons for hybrid layer degradation.…”