Effect of Density of Helicotylenchus dihystera on Growth of Solanum lycopersicum

Kim, Donggeun; Ryu, Younghyun; Lee, Youn-Su; Choi, Insoo; Hu, Changsuk

doi:10.5423/rpd.2014.20.2.107

Cited by 4 publications

(4 citation statements)

References 5 publications

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“…infestation. The infestation of Helicotylenchus dihystera resulted in poor plant height as reported by Kim et al, 2014 in case of their study on tomatoes 15 . They observed declined plant height as a result of the infestation.…”

Section: Resultsmentioning

confidence: 80%

Identification and Management of Plant Parasitic Nematodes Associated with Walnut (Juglans Regia L.) in Abbottabad

Saeed¹,

Anar²,

Saeed³

et al. 2022

PJMHS

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Purpose: Phytonematodes known as the hidden enemies of plants and crops are potential pest of walnut trees in Abbottabad, KP Pakistan. Methods: Soil samples from walnut trees were collected from different localities were processed by using Bearmann funnel techniques. Several nematode species were identified including Meloidogyne incognita, Helicotylenchus dihystera, Psilenchus hilarulus, Tylenchus sp. and Helicotylenchus vulgaris. Findings & Practical implication: Management of plant parasitic nematodes by using castor oil cakes (8kg/tree), cow dung (8 kg/tree) and duck manure (8kg/tree) showed that population was declined after application. Plant growth and yield was also improved. Data plotted in histogram graphs and t-test showed that p values were found significant. Conclusion: The mean and S.D values of initial population, 3 month, 6 month and 12 month for castor oil cakes are 116 ± 1.2, 84 ± 1.0, 60 ± 1.0, 42 ± 0.6, for cow dung 127 ± 5.7, 110 ± 2.4, 87 ± 2.4, 73 ± 1.3 and for duck manure 108 ± 2.4, 92 ± 1.0, 69 ± 0.5, 45 ± 1.0. Keywords: Phytonematodes, walnut, castor oil, cow dung, duck manure.

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Section: Resultsmentioning

confidence: 80%

Identification and Management of Plant Parasitic Nematodes Associated with Walnut (Juglans Regia L.) in Abbottabad

Saeed¹,

Anar²,

Saeed³

et al. 2022

PJMHS

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show abstract

“…For example, in Turkey, H. digonicus , H. tunisiensis and H. varicaudatus have been reported from tomato rhizosphere soils [ 25 ]. Kim et al (2014) reported that H. dihystera can suppress the growth of tomato and this species was also reported from tomato in China [ 26 ]. H. thornei Roman, 1965 is also described from soil around the roots of tomato in Ludhiana, India [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and a culture method for a Helicotylenchus microlobus from tomato in China

Xia¹,

Li²,

Xu³

et al. 2022

BMC Zool

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Background The nematodes of the genus Helicotylenchus are root parasites of a wide variety of plants, and certain species can cause serious damage to their hosts. During a survey of the plant-parasitic nematode associated with tomato, a population of Helicotylenchus was collected from tomato roots and soil samples. Thus, one of the objectives of the study was to confirm the specie of Helicotylenchus obtained from the tomato samples based on morphological and molecular characteristics. In addition, a mass pure culture of plant-parasitic nematodes is key to pathogenicity studies and many other biological studies. However, a successful mass rearing method for Helicotylenchus has not been reported. Thus, the other objective of the study was to establish a method of culturing Helicotylenchus. Results Based on both the morphological characteristics and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA (rRNA) sequences the specimens were identified as Helicotylenchus microlobus. Phylogenetic analysis with the rRNA sequences of the ITS and 28S D2-D3 regions was consistent with molecular identification, suggesting this population formed a highly supported clade with other H. microlobus populations. Additionally, a method for culture of H. microlobus on carrot disks was established, and the effect of temperature on the reproduction rate (Rr) of H. microlobus was investigated. The optimum temperature for culturing H. microlobus on carrot disks was 27.5 °C and, after inoculation with 30 females of H. microlobus at 27.5 °C for 90 days, Rr reached 406. Conclusions To our knowledge, this is the first detailed description of H. microlobus from tomato in China. This study also demonstrated that the carrot disk method is suitable for the culture of H. microlobus. This study lays a foundation for other related research on H. microlobus, and has significance for the study of Helicotylenchus.

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“…For example, in Turkey, H. digonicus, H. tunisiensis and H. varicaudatus have been extracted from tomato rhizosphere soils [24]. Kim et al (2014) reported that H. dihystera can suppress the growth of tomato [25]. H. thornei n. sp.…”

Section: Discussionmentioning

confidence: 99%

“…The reason for differences may be related to underlying genetic variation and geographical position. In addition, spiral nematodes are a genera of plant parasitic nematodes known to contain various species complexes that normally exhibit similar diagnostic characteristics [27]. However, species delineation within the genus is not always reliable due to enormous intraspeci c variability in characters apparently in uenced by environmental conditions [28].…”

Section: Discussionmentioning

confidence: 99%

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

Xia

et al. 2022

Preprint

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Background: Spiral nematodes of the genus Helicotylenchus are globally distributed, and contain various species complexes that normally exhibit similar diagnostic characteristics. Some studies on the parasitism of tomato by spiral nematodes include only census data, or morphological data and lack detailed molecular data in China. At present, carrot disks have been successfully applied in the culture of plant-parasitic nematodes, but carrot disks are not suitable for rearing all migratory plant-parasitic nematodes, and only a small number of plant-parasitic nematodes can be cultured. However, the establishment of a culture method for Helicotylenchus has not been reported.Results: Spiral nematodes were recovered from tomato roots and soil samples, and the purified spiral nematode LQ-1 population was identified based on morphological and molecular biological technology. Both the morphological characteristics and molecular analysis of the internal transcribed spacer (ITS) and D2-D3 expansion region of 28S ribosomal RNA sequences revealed that the species of the spiral nematode LQ-1 population was Helicotylenchus microlobus. Phylogenetic analysis with the rRNA sequences of the ITS and 28S D2-D3 regions was consistent with molecular identification, suggesting that the spiral nematode LQ-1 population formed a highly supported clade with other H. microlobus populations. Additionally, a method for culture of H. microlobus on carrot disks was established, and the effect of temperature on the reproduction rate (Rr) of this nematode was investigated. The optimum temperature for H. microlobus culture on carrot disks was 27.5 °C, and the Rr reached 406 after 90 days of inoculation with 30 females.Conclusions: To our knowledge, this is the first systematic description of H. microlobus from tomato in China. This study also demonstrated for the first time that the carrot disk method is suitable for the culture of H. microlobus. This study lays a foundation for other related research on H. microlobus, and has significance for the study of spiral nematodes.

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Effect of Density of Helicotylenchus dihystera on Growth of Solanum lycopersicum

Cited by 4 publications

References 5 publications

Identification and Management of Plant Parasitic Nematodes Associated with Walnut (Juglans Regia L.) in Abbottabad

Identification and Management of Plant Parasitic Nematodes Associated with Walnut (Juglans Regia L.) in Abbottabad

Identification and a culture method for a Helicotylenchus microlobus from tomato in China

Identification and establishment of a culture method for the spiral nematode Helicotylenchus microlobus from tomato in China

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