Effect of dendroaspis natriuretic peptide (DNP) on L-type calcium channel current and its pathway

Zhang, Shuying; Cai, Zhengxu; Li, Ping; Cheng, Can; Qu, Cheng-Long; Guo, Huishu

doi:10.1016/j.regpep.2010.05.015

Cited by 2 publications

(1 citation statement)

References 20 publications

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“…However, this downstream signaling is not directly modulated by NO itself in the cardiomyocytes but occurred in cardiac fibroblasts (Menges et al, 2019). The Dendroaspis natriuretic peptide (DNP) is an example of the snake toxins which accelerate the GC-cGMP-PKG activity and, hence, inhibits the LTCC (Zhang et al, 2010). Notexin, the myotoxic phospholipase acquired from the tiger snake (Notechis scutatus scutatus), causes skeletal muscle injury via the increased production of free radical NOs that induces the apoptotic process .…”

Section: Other Possible Pathways Underlying ß-Ctx's Effectmentioning

confidence: 99%

Studies of beta-cardiotoxin from king cobra venom on rat cardiac functions: Effect on isolated single cardiomyocyte

Lertwanakarn¹

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Beta-cardiotoxin (ß-CTX), a novel protein isolated from the King cobra (Ophiophagus hannah) venom has previously been proposed as a beta-blocker candidate. However, cellular mechanisms of ß-CTX on cardiomyocyte is unknown. This study aimed to evaluate the impact of ß-CTX on isolated rat cardiomyocyte function and the cardiac myofibrillar activity, and to explore involvement of ß-adrenergic receptor (ß-AR) signaling pathway. ß-CTX was isolated and purified using two-step chromatographic method and confirmed by N-terminal sequencer. The function of ß-CTX on cardiomyocyte was compared to propranolol in 3 conditions, basal state, with isoproterenol (ISO), and with forskolin (FSK). ß-CTX exhibited more potency than propranolol to suppress the myocyte contraction without altering calcium transient. However, ß-CTX prolonged the calcium decaying resulted in negative lusitropy. Although cardiac functions were blunted by ß-CTX in the presence of either ISO or FSK, the phosphorylation of the downstream ß-AR signaling sites were not affected. The compound was further tested on the cardiac myofibrillar ATPase activity and the kinetics. ß-CTX suppressed the maximal ATPase activity without changing Ca2+-sensitivity of the myofibril. In conclusion, the mechanism of ß-CTX on cardiomyocyte was not mediated through classical ß-AR pathway but directly suppress the actomyosin ATPase activity, depress the myofilament kinetics, and hence, reduce the cardiomyocyte functions.

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Section: Other Possible Pathways Underlying ß-Ctx's Effectmentioning

confidence: 99%

Studies of beta-cardiotoxin from king cobra venom on rat cardiac functions: Effect on isolated single cardiomyocyte

Lertwanakarn¹

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Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway

Idrizaj

Garella

Nistri

et al. 2020

IJMS

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Some adipokines, such as adiponectin (ADPN), other than being implicated in the central regulation of feeding behavior, may influence gastric motor responses, which are a source of peripheral signals that also influence food intake. The present study aims to elucidate the signaling pathways through which ADPN exerts its actions in the mouse gastric fundus. To this purpose, we used a multidisciplinary approach. The mechanical results showed that ADPN caused a decay of the strip basal tension, which was abolished by the nitric oxide (NO) synthesis inhibitor, L-NG-nitro arginine (L-NNA). The electrophysiological experiments confirmed that all ADPN effects were abolished by L-NNA, except for the reduction of Ca2+ current, which was instead prevented by the inhibitor of AMP-activated protein kinase (AMPK), dorsomorphin. The activation of the AMPK signaling by ADPN was confirmed by immunofluorescence analysis, which also revealed the ADPN R1 receptor (AdipoR1) expression in glial cells of the myenteric plexus. In conclusion, our results indicate that ADPN exerts an inhibitory action on the gastric smooth muscle by acting on AdipoR1 and involving the AMPK signaling pathway at the peripheral level. These findings provide novel bases for considering AMPK as a possible pharmacologic target for the potential treatment of obesity and eating disorders.

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Effect of dendroaspis natriuretic peptide (DNP) on L-type calcium channel current and its pathway

Cited by 2 publications

References 20 publications

Studies of beta-cardiotoxin from king cobra venom on rat cardiac functions: Effect on isolated single cardiomyocyte

Studies of beta-cardiotoxin from king cobra venom on rat cardiac functions: Effect on isolated single cardiomyocyte

Adiponectin Exerts Peripheral Inhibitory Effects on the Mouse Gastric Smooth Muscle through the AMPK Pathway

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