Effect of Dendranthema indicum Extracts on Cell and DNA Damage Induced by Oxidative Stress

Abstract

In this study, we evaluated the protective effects of ethanol extracts from Dendranthema indicum on cell and DNA damages induced by oxidative stress. Antioxidant activities of D. indicum extracts are higher than scavenging activities of DPPH free radical and hydroxyl radical by 92.8% and 73.8%, respectively, and higher than ferrous iron chelating effects by 59.4%. D. indicum extracts showed a protective effect on oxidative cell damage by inhibiting lipid peroxidation by 90.3% in the control group, and inhibiting expression level of p21 protein by 79.6% for the control group. This means D. indicum extracts have a great protective effect against oxidative stress. DNA fragmentation inhibition in D. indicum extracts were 89.6% for the control group, which makes the movement of DNA tail reduced, and phosphorylation of H2AX was 20.2% of the radical experiment group. This means that D. indicum extracts effectively inhibit DNA fragmentation and H2AX phosphorylation. Taken together, we suggest that ethanol extract from D. indicum has a role as a useful chemopreventor against oxidative damage.

Validation of Natural Cosmetic Resources Containing EtOH Extracts of Chrysanthemum indicum and Cymbopogon cirtratus by the Simultaneous Analysis of Their Marker Compounds Using High Performance Liquid Chromatography-Diode Array Detector

“…한방에서 열감기, 폐렴, 기관지염, 두통, 위염, 장기, 종기 등의 치료에 처방한다. 보고된 활성으로는 노화와 주름 생성 경 감 (Kim, 2016) 항암 활성, 산화스트레스 저해, 항산화 활성, 결핵균 및 바이러스 등에 대한 항박테리아 효과 등이 보고되어 있다 (Kim et al, 2005;Park et al, 2011;Woo et al, 2010). 감국의 성분으로는 eugenol, isoeugenol, cynaroside, apigetrin 등이 보고되어 있다 (Hyun et al, 2019 (So et al, 2019), 항산화, 지방세포 분화 억제 효과, 항암, 항염증 활성 등이 보고 되어있으며 (Bhattacharya, 1998;Francisco, 2014), 성분으로는 α-pinene, camphene, limonene, myrcene, citral 등이 보고 되어있다 (Jo et al, 2019 Figure 1A) 과 레몬그라스 추출물에서 PA의 HPLC 프로파일( Figure 1B), 지표 성 분의 구조( Figure 1C)를 Figure 1에 나타내었다 (Kim, 2016).…”