Effect of Delta‐9‐tetrahydrocannabinol Administration on the Lipid Constituents of Rat Brain Subcellular Fractions

Sarkar, Chitta P.; Ghosh, J. J.

doi:10.1111/j.1471-4159.1975.tb11891.x

Cited by 16 publications

(3 citation statements)

References 29 publications

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“…Similarly, acute treatment led to a marked increase in sialoglyco-proteins and ganglioside content in both microsomal and synaptosomal fractions, but the effects were not observed after chronic treatment (Sarkar and Ghosh 1976). Sarkar and Ghosh (1975) also investigated the effect of THC on lipid constituents of subcellular fractions in rat brain. Their results have been summarised in table 11.…”

Section: Cannabismentioning

confidence: 99%

Structure-Activity Relationships of Tricyclic and Nonclassical Bicyclic Cannabinoids

Mr²

1987

PsycEXTRA Dataset

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The Research Analysis and Utilization System (RAUS) is designed to serve four functions: Collect and systematically classify the findings of all intramural and extramural research supported by the National Institute on Drug Abuse (NIDA); Evaluate the findings in selected areas of particular interest and formulate a state-of-the-art review by a panel of scientific peers; Disseminate findings to researchers in the field and to administrators, planners, instructors, and other interested persons; Provide a feedback mechanism to NIDA staff and planners so that the administration and monitoring of the NIDA research program reflect the very latest knowledge gleaned from research in the field. Since the number of research topics that can be intensively reviewed annually is limited, four subject areas are chosen each year to undergo a thorough examination. Distinguished scientists are invited to participate. Each scientist is provided reports from NIDA-funded research and asked to add information derived from the literature and his or her own research and to prepare a comprehensive, state-of-the-art review paper on an assigned topic. These papers, together with a summary of the discussions which take place at the review meeting, make up a RAUS Review Report in the NIDA Research Monograph series. In recent years a large number of reviews and books have appeared summarizing research advances in the chemistry, pharmacology, toxicology, metabolism, and distribution of cannabinoids and their synthetic analogs. Yet the volume of research on structure-activity relationships of cannabinoids appears to have decreased. In order to evaluate NIDA's current research program on cannabinoids, to rekindle interest in this critical area, and to focus attention on profitable avenues for future research, NIDA found it timely to bring together experts on the structure-activity relationships of cannabinoids for a comprehensive review. v

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Section: Cannabismentioning

confidence: 99%

Structure-Activity Relationships of Tricyclic and Nonclassical Bicyclic Cannabinoids

Mr²

1987

PsycEXTRA Dataset

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“…Brains from 2 to 5 rats were pooled, blotted, and homogenized in 9 vol of 0.32 M sucrose, using a Potter-Elvehjem Teflonglass homogenizer with 10 complete upand-down strokes at 0-4°C. Subcellular fractions were prepared by the method of Whittaker and Barker (1972) as described previously (Sarkar and Ghosh, 1975). Fraction Pz (crude mitochondria) was resuspended and layered on a discontinuous sucrose gradient consisting of equal volumes of 0.8 M sucrose and 1.2 M sucrose.…”

Section: Methodsmentioning

confidence: 99%

“…The purity of the subcellular fractions obtained by this fractionation procedure was established earlier (Sarkar and Ghosh, 1975) by assays of each fraction for marker enzyme activities; i.e., succinic dehydrogenase for mitochondria, glucose-6-phosphatase for microsomes, and acetylcholine esterase for synaptosomes. The purity of subcellular fractions was confirmed in the present study by electron microscopy.…”

Section: Methodsmentioning

confidence: 99%

Studies on the Mechanism of the Epileptiform Activity Induced by U18666A. I. Gross Alteration of the Lipids of Synaptosomes and Myelin

1982

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U18666A, an inhibitor of desmosterol reductase (a terminal enzyme in cholesterol synthesis), has been found to produce chronic epileptiform activity in laboratory animals. Since desmosterol might substitute for cholesterol in neuronal membranes without detriment, the present study was undertaken to examine the possibility that this drug-induced epilepsy was related to changes in other brain lipids. Chronic treatment of rat with U18666A, beginning at one day of age, resulted in pronounced decreases in the concentration of phospholipids and increases in gangliosides of brain microsomal, synaptosomal, and crude myelin fractions. Since total sterol levels were not changed, the ratio of sterols to phospholipids also increased. If drug treatment was stopped at 4 weeks of age, brain lipids of all subcellular fractions examined returned to normal levels by 8 weeks, and no epileptiform activity was detected. However, following 8 weeks of continuous treatment, epileptiform activity was present, and the changes in brain lipids were focused in the myelin fraction. Phospholipid levels and the sterol:phospholipid ratio of microsomes and synaptosomes, in contrast to myelin, were near normal; however, gangliosides were still clearly elevated in all fractions. A reported ability to induce epileptiform activity in rats by treatment with antiserum to brain gangliosides could indicate a special significance of the altered myelin and synaptic gangliosides to the U18666A-induced epilepsy. We suggest that some epileptiform conditions could be directly related to alterations in the lipid composition of critical neuronal structures.

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Effect of cannabis extract on uterine glycogen metabolism in prepubertal rats under normal and estradiol-treated conditions

Chakravarty

Ghosh

1977

Biochemical Pharmacology

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Effect of Delta‐9‐tetrahydrocannabinol Administration on the Lipid Constituents of Rat Brain Subcellular Fractions

Cited by 16 publications

References 29 publications

Structure-Activity Relationships of Tricyclic and Nonclassical Bicyclic Cannabinoids

Structure-Activity Relationships of Tricyclic and Nonclassical Bicyclic Cannabinoids

Studies on the Mechanism of the Epileptiform Activity Induced by U18666A. I. Gross Alteration of the Lipids of Synaptosomes and Myelin

Effect of cannabis extract on uterine glycogen metabolism in prepubertal rats under normal and estradiol-treated conditions

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